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Method for analyzing and determining perchlorate in food by utilizing DLLME and digital image of scanner

A digital image, perchlorate technology, applied in the direction of analyzing materials, material analysis by optical means, instruments, etc., to achieve the effect of low cost of instruments and high application prospects

Inactive Publication Date: 2019-08-23
NORTHWEST NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Digital photography and scanned images are often quantified using the RGB color space system, but so far it has not been used for the detection of perchlorate

Method used

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  • Method for analyzing and determining perchlorate in food by utilizing DLLME and digital image of scanner
  • Method for analyzing and determining perchlorate in food by utilizing DLLME and digital image of scanner
  • Method for analyzing and determining perchlorate in food by utilizing DLLME and digital image of scanner

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Experimental program
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Effect test

Embodiment 1

[0024] The detection of perchlorate content in embodiment 1, milk

[0025] (1) Milk sample pretreatment: Add 5.0 mL milk solution into a 50 mL polypropylene conical tube, add 5.0 mL 1.0% acetic acid aqueous solution and 20.0 mL acetonitrile. Cover the centrifuge tube cap and shake vigorously for 5 minutes. The mixture was centrifuged at 6000 rpm for 20 min. Use a nylon syringe filter with a pore size of 0.20 μm to take the supernatant. Before measurement, filter all digested samples through ultrafiltration membranes with a pore size of 4 nm;

[0026] (2) ClO 4 - Detection: Add 0.5-6mL sample to a 15mL centrifuge tube, add 0.4mL 0.10g·L -1methylene blue solution, then add 1.0mL HAc-NaAc buffer solution (pH=4.5), dilute to 10mL, quickly inject 600μL dichloromethane and methanol (v:v=2:1) ​​solution into the target analysis via a micro syringe to induce the formation of a turbid solution, and the mixture was shaken vigorously on a vortex shaker for 2 min. Use a centrifuge ...

Embodiment 2

[0027] Embodiment 2, the detection of perchlorate content in tap water

[0028] (1) Obtained in the laboratory and analyzed directly without any pretreatment.

[0029] (2) ClO 4 - Detection: Add 1-6mL sample to a 15mL centrifuge tube, add 0.4mL 0.10g·L -1 methylene blue solution, then add 1.0mL HAc-NaAc buffer solution (pH=4.5), dilute to 10mL, quickly inject 600μL dichloromethane and methanol (v:v=2:1) ​​solution into the target analysis via a micro syringe to induce the formation of a turbid solution, and the mixture was shaken vigorously on a vortex shaker for 5 min. Use a centrifuge to centrifuge at 4000rpm for 5min, remove the upper aqueous phase; use a micro-sampler to take 4.0 μL of the enriched phase point on inkjet printing paper, and use a scanner to scan the sample piece of paper after the solvent is air-dried, and then use GIMP and ImgeJ software software to convert the scanned image into a grayscale mode, select the grayscale sample spot area area to integrate...

Embodiment 3

[0030] The detection of perchlorate content in embodiment 3, spinach

[0031] (1) Sample pretreatment: Dry the sample at 120°C, grind it into fine powder in a mortar, and sieve it with 80 mesh. Put 10.0g of dried spinach sample into a 100mL Erlenmeyer flask, add 30mL of concentrated nitric acid, and place it at room temperature for 20 hours for digestion. The second is to add 10mL of concentrated nitric acid and hydrogen peroxide dropwise, and heat at 150°C on an electric heating plate to drive out the nitric acid. Repeat the process until the solution is clear. After the solution is cooled, add secondary water to make up to volume.

[0032] (2) ClO 4 - Detection: Add 0.5-6mL sample to a 15mL centrifuge tube, add 0.4mL 0.10g·L -1 methylene blue solution, then add 1.0mL HAc-NaAc buffer solution (pH=4.5), dilute to 10mL, quickly inject 600μL dichloromethane and methanol (v:v=2:1) ​​solution into the target analysis via a micro syringe to induce the formation of a turbid so...

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Abstract

The invention discloses a method for analyzing and determining perchlorate in water samples and food by utilizing a digital image of a scanner. In an acidic system with the pH value of 4.5, a methylene blue (MB) color developing agent can react with ClO4<-> to generate a hydrophobic green ion association complex; then methanol is used as a dispersing agent, dichloromethane is used as an extractingagent, and the DLLME technology is utilized to enrich the colored ion association complex into organic phase dichloromethane; then sampling the colored complex in the organic phase is performed, spotting the colored complex on ink-jet printing paper is performed, the ink-jet printing paper is scanned by a household scanner, an image is converted into a gray mode by using a GIMP and an ImgeJ, a gray area region is selected for integration to obtain the integrated optical density, and a quantitative relationship is established between the integrated optical density and the concentration of ClO4<->. The method provided by the invention uses the ink-jet printing paper as a color developing substrate to obtain the color quantitative data, the image capturing operation is very little influencedby the ambient light, and the method provided by the invention has the advantages of rapidness, accuracy and sensitivity, and is mainly used for detecting ClO4<->.

Description

technical field [0001] The invention relates to a fast determination method for perchlorate, in particular to a method for determining the content of perchlorate in water samples and food by combining DLLME technology and scanner digital image analysis technology, and belongs to the technical field of food detection. Background technique [0002] Due to its special chemical and physical properties, such as high water solubility, fluidity and stability, perchlorate is regarded as a new type of persistent organic pollutants. Its main pollution sources can be divided into two categories, artificial aspects and natural aspects. Man-made perchlorate compounds are often derived from military and chemical products, including solid propellants, rocket fuels, ammunition, pyrotechnics, commercial explosives, and automotive air bags, among others. Trace amounts of perchlorate are also found in environmental water samples and food, especially in beverages, drinking water and food. Dri...

Claims

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Application Information

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IPC IPC(8): G01N21/84
CPCG01N21/84
Inventor 彭波樊祢祢黎凯李婷婷徐佳美郭阳
Owner NORTHWEST NORMAL UNIVERSITY
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