Cell microsphere array chip device and method for measuring potential signal
A signal measurement and microsphere array technology, applied in the field of biomedicine, to achieve the effects of high repeatability and flexibility, high measurement signal-to-noise ratio, and convenient operation
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Embodiment 1
[0038] figure 1 The chip device with a linear structure designed for it includes a cell microsphere flow channel 1, a cell microsphere capture channel 2, a separate potential signal measurement electrode 3 and an external detection system 4, and is mainly used for testing cardiomyocyte microspheres. The local field potential of the cell, adapted to a cardiomyocyte microsphere size of 200 μm.
[0039]The cell microsphere flow channel is the flow channel of the cardiomyocyte microsphere suspension, and the width and height of the channel are both 250 μm. The cardiomyocyte microsphere suspension flows from left to right, and the suspension can be recovered at the end of the channel.
[0040] The microsphere capture channel includes a microsphere inlet, a microsphere capture fixed position, and a liquid outlet, and its channel is directly connected with the cell microsphere flow channel, which is a branch of the flow channel, and the number of channels is 6. The channel size of ...
Embodiment 2
[0044] A preparation method of the aforementioned chip device for cell microsphere potential signal analysis. The cell microsphere flow channel and the capture channel are made by soft photolithography technology, and form a two-layer structure with the glass sealing layer on the other side. This embodiment The preparation method is mainly suitable for the microfluidic chip for testing the potential signal of cell microspheres with a size below 200 μm.
[0045] In the manufacturing process of this embodiment: firstly, SU8-3025 photoresist (negative resist) is dropped on the surface of the silicon wafer to uniform the resist, rotate at 500 rpm for 10 seconds, and rotate at 100 rpm for 30 seconds. Then, pre-bake at 65 degrees Celsius for 1 minute, and at 95 degrees Celsius for 5 minutes; carry out ultraviolet exposure (150-250mJ / cm 2 ); after the exposure, medium bake at 65°C for 1 minute, and 95°C for 6 minutes; cool to room temperature, and develop with the developer for about...
Embodiment 3
[0048] A preparation method of the aforementioned chip device for cell microsphere potential signal analysis, the steps are as follows: draw the designed graphics with CAD software, the graphics width is 800 microns, and import them into an infrared cutting machine. Corresponding channels were cut on a PMMA substrate with a thickness of 1 mm. The PMMA substrate containing channels is pasted on a glass or PMMA substrate, and then the upper surface is covered with PMMA or glass. Clamp the entire three-layer structure with screws. Fix the detection electrode (3 shown in the drawing) at the corresponding part of the capture channel (2 shown in the drawing), and the desired PMMA multi-channel chip can be obtained.
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