Method for preparing DEHP degrading bacterial agent and method for effectively reducing DEHP pollution in vegetable production

Abstract

The invention discloses a method for preparing DEHP degrading bacterial agent and a method for effectively reducing DEHP pollution in vegetable production. The bacterial agent is prepared by the steps: activating rhodococcus sp. 2G and then carrying out fermented culture, centrifuging the fermentation liquid, collecting bacteria, adding a protective agent to the bacteria, pre-freezing, and carrying out vacuum freeze-drying of the pre-frozen bacteria to obtain the DEHP degrading bacteria freeze-dried bacterial agent. A certain proportion of the bacterial agent is added in a farmland having soilpolluted by the DEHP, so the accumulation amount of the DEHP in planted vegetables is reduced while the DEHP in the soil is removed; by addition of the DEHP degrading bacterial agent and colonizationof the degrading bacteria at vegetable rhizospheres, the residual amount of the DEHP in the polluted soil and the planted vegetables can be effectively reduced. The method not only remediates the DEHP polluted soil in farmland, but also produces agricultural products safely, namely a concept of remediating while producing, has the advantages of high treatment efficiency, low cost and no secondarypollution, and has important application prospects in prevention and control of organic pollution in the soil of the farmland.

Description

technical field [0001] The invention relates to the technical field of biological treatment of environmental pollutants, in particular to a preparation of a DEHP-degrading bacterial agent and a method for effectively reducing DEHP pollution in vegetable production. Background technique [0002] Phthalates (PAEs) are often used as plasticizers in the industry and are widely used in plastics or polymer materials to improve the flexibility and durability of plastic products. Since it is not connected with plastic molecules through chemical bonds, PAEs can break away from the plastic body and enter the outside world over time, causing environmental pollution. At present, PAEs have become one of the most common chemical substances that people are exposed to daily, and can enter the human body through diet and skin contact, posing a threat to human health. Studies have shown that bis(2-ethylhexyl) phthalate (DEHP) is the most abundant PAEs compound in the environment, and it is l...

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Problems solved by technology

[0003] Patents CN201510066083.9 and CN201510066129.7 disclose a strain of Rhodococcus sp.2G that can simultaneously degrade various phthalates and its application in degrading phthalates, using various PAEs as the only Carbon source and energy for growth and reproduction. Under pure culture conditions, the bacteria can degrade 200mg / kg of mixed PAEs (respectively containing 200mg / kg DMP, DEP, DBP and DEHP) in the inorganic salt medium for 7 days. The degradation rate is uniform. More than 97%, but the application research on this strain is limited to the shake flask degradation research in the laboratory, and has not been applied to the actual treatment of polluted farmland soil. At the same time, how to prepare Rhodococcus sp.2G can be promoted in production The applied bacterial agent needs to be further studied

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