Trypsin precursor gene and its encoded protein, interfering RNA and application thereof

A technology of trypsin and gene encoding, which is applied in the trypsin precursor gene and its encoded protein, interfering RNA and application fields. The effect of longevity
CN110195073AInactive Publication Date: 2019-09-03HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2019-09-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a trypsin precursor gene and its encoded protein, interfering RNA and an application thereof, which belong to the technical field of insect gene engineering. A cDNA sequence ofthe trypsin precursor gene is shown as SEQ ID NO: 1; an amino acid sequence of the protein is shown as SEQ ID NO: 2; a nucleotide sequence of the interfering RNA is shown as SEQ ID NO: 3; the expression level of the trypsin precursor gene in adelphocoris suturalis in Miridae is reduced, the quantity of eggs in the adelphocoris suturalis ovary and the final production of eggs can be significantly reduced, and the life span of female adults can be shortened. The application provided by the invention can eventually lead to the decline of the population development, provides a new idea for controlling the development of the adelphocoris suturalis population, and also provides a theoretical basis for the realization of adelphocoris suturalis and other hemipteran insects in green control.
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Description

technical field

[0001] The invention belongs to the technical field of insect genetic engineering, and in particular relates to a trypsin precursor gene and its encoded protein, interference RNA and application. Background technique

[0002] Adelphocoris suturalis belongs to Hemiptera Miridae. It is distributed in North Korea, Japan, eastern and coastal areas of Siberia, and the Caucasus. It is mainly concentrated in the Yangtze River Basin and Yellow River Basin in China (Jiang Yuying et al., Regional monitoring and management of Lygus bugs. 2015, Beijing: Beijing China Agricultural Press). Since the planting of Bt cotton in 1997, Lygus chinensis has changed from a minor pest to a major pest, and Lygus medusa is one of the main pests in cotton planting areas in the Yangtze River Basin of my country (Lu Yanhui et al., Research progress on comprehensive management of Lygus cotton. Plant Protection, 2007.06,10-15; Wu K, Lu YH, Feng HQ, Jiang YY, Zhao JZ, 2008.Suppression of Co...

Claims

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