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A kind of highly stable immobilized carbonic anhydrase and its preparation method and application

A carbonic anhydrase and immobilized enzyme technology, applied in the field of biological enzyme treatment of air pollutants, to achieve the effects of large load rate, improved apparent catalytic rate, and high activity retention rate

Active Publication Date: 2021-05-11
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Relevant researchers have selected porous materials (molecular sieve SBA-15, porous glass, activated carbon, etc.) and non-porous nanomaterials (TiO 2 , SiO 2 , SiO 2 -ZrO 2 , Bionic SiO 2 ) etc. as immobilized carriers, the thermal stability and chemical stability of carbonic anhydrase are all improved to a certain extent, but after utilizing the above-mentioned carriers for immobilization, the enzyme load (20-50mg / g) and activity retention (30-50% ) lower

Method used

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  • A kind of highly stable immobilized carbonic anhydrase and its preparation method and application
  • A kind of highly stable immobilized carbonic anhydrase and its preparation method and application
  • A kind of highly stable immobilized carbonic anhydrase and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Example 1 Preparation of immobilized carbonic anhydrase CA / ZIF-8

[0036] Dissolve 3 g of carbonic anhydrase in 100 ml, 10 mg / mL polyvinylpyrrolidone (PVP) aqueous solution to prepare a 30 mg / mL carbonic anhydrase solution to synthesize immobilized enzyme particles CA / ZIF-8.

[0037]Add 2mL of zinc nitrate aqueous solution (0.31mol / L) dropwise to 25mL of 2-methylimidazole aqueous solution (1.25mol / L), and stir at 30°C for 2.5min at 200rpm, then add 1.2mL of carbonic anhydrase aqueous solution (30mg / mL) was added to the reaction solution, and stirring was continued for 27.5min. After the reaction was completed, the samples were collected by centrifugation at a speed of 6500 rpm for 8 minutes. And washed three times with deionized water to remove unimmobilized carbonic anhydrase, and dried under vacuum at 30°C to obtain immobilized enzyme particles CA / ZIF-8. The loading rate of the immobilized enzyme particles was 15.4%. Its PXRD pattern and scanning electron microscop...

Embodiment 2 2

[0038] Embodiment 2 carbon dioxide absorption experiment

[0039] The immobilized carbonic anhydrase granule CA / ZIF-8 prepared in Example 1, methyldiethanolamine (MDEA) and water are mixed and formulated into a 20mL absorbent, wherein the concentration of MDEA is 1mol / L, and the absorbent is internally immobilized The concentration of carbonic anhydrase is 6.7g / L, and the CO 2 The absorption rate is 2.48E -6 kmol / (m 2 s), reaching 75% of the 1mol / L MEA solution.

Embodiment 3 2

[0040] Embodiment 3 carbon dioxide absorption experiment

[0041] The immobilized carbonic anhydrase particles and 20wt% potassium carbonate-potassium bicarbonate buffer solution (pH=10.3) were mixed to prepare 20 mL of absorbent, and the concentration of immobilized carbonic anhydrase in the absorbent was 6.7 g / L. Experimentally measured CO 2 The absorption rate is 8.09E -7 kmol / (m 2 s), reached 25% of the 1mol / L MEA solution.

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Abstract

The invention discloses a highly stable immobilized carbonic anhydrase and its preparation method and application. The preparation method of described highly stable immobilized carbonic anhydrase is: mix 2-methylimidazole solution and zinc nitrate hexahydrate solution, at 25-35 ℃, add carbonic anhydrase solution after stirring for 0-5min and continue to stir, react After completion, the obtained reaction solution is post-treated to obtain immobilized carbonic anhydrase CA / ZIF‑8. The immobilized carbonic anhydrase CA / ZIF‑8 is applied to the preparation of CO 2 absorbent. The present invention adopts "one-pot method" to prepare under normal temperature conditions, has simple operation, short reaction time, and is easy to popularize. Meanwhile, its mild reaction conditions will not significantly affect the structure of carbonic anhydrase, so that its activity retention rate is relatively high. The obtained immobilized carbonic anhydrase has good stability and reusability. It still retains 99% of the initial activity after being stored at high temperature for 3 days, and still retains 84% ​​of the initial activity after repeated use for 4 times, which can reduce the cost of replacing the immobilized enzyme. the cost of.

Description

technical field [0001] The present invention relates to a highly stable immobilized carbonic anhydrase and its preparation method, in particular, it proposes to use a metal organic framework as a protective layer to prepare the immobilized enzyme, which is suitable for capturing carbon dioxide in the flue gas of a coal-fired power plant and belongs to air pollution The technical field of biological enzyme treatment of substances. Background technique [0002] Greenhouse gases in the atmosphere absorb long-wave radiation from the ground, causing the earth's surface temperature to rise continuously, triggering a series of harsh natural phenomena such as melting glaciers, rising sea levels, and extreme weather. According to the statistics department, the concentration of carbon dioxide in the atmosphere has increased by 40% compared with pre-industrialization, and is increasing at a rate of 18ppb per year, making the global temperature increase by 0.3°C per decade. Carbon diox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14B01D53/14
CPCB01D53/1475B01D53/1493C12N9/88C12N11/14C12Y402/01001Y02C20/40
Inventor 张士汉杜敏娥邵培静叶杰旭陈建孟
Owner ZHEJIANG UNIV OF TECH