Selenium-rich oyster micromolecule protein peptide solid beverage and preparation method thereof

[0059] Example 1:

[0060] This embodiment provides a selenium-enriched oyster small molecule protein peptide solid beverage, which includes the following parts by weight: 50 parts by weight of selenium-enriched oyster small molecule protein peptide, 16 parts by weight of selenium-enriched cooked wheat flour, spiral 0.5 parts by weight of algae flour, 4 parts by weight of konjac flour, 5 parts by weight of oligofructose, 4 parts by weight of vegetable protein, 2 parts by weight of cooked quinoa flour, 18 parts by weight of skimmed milk powder, 0.5 parts by weight of complex minerals, 0.5 parts by weight of complex vitamins The solid beverage is sterilized by the following mixture. In the present invention, the selenium-enriched oyster small molecule protein peptide can quickly supplement the nutrients needed in the body, and can hardly consume energy in the body to reach the required nutritional position. The source is not particularly limited, and conventional commercial products in the field can be used, or they can be prepared from selenium-enriched oysters through cleaning, crushing, steaming, enzymatic hydrolysis, filtration, concentration, and drying. The solid beverage of the present invention uses selenium-enriched oyster small molecule protein peptides and selenium-enriched cooked wheat flour as the main raw materials, and is mixed with spirulina powder, konjac flour, oligofructose, vegetable protein, cooked quinoa flour, skimmed milk powder, complex minerals and Compound vitamins and other ingredients make the solid beverage of the present invention have a comprehensive, balanced and reasonable nutrient composition, good satiety, good effect, quick effect, ready-to-eat preparation, good for body absorption, good for helping people to shape their bodies, and good To meet people's requirements for a perfect body, the solid beverage of the present invention is rich in protein, vitamins and selenium, and has high nutritional value.

[0061] The compound vitamin is a mixture of vitamin A, vitamin D, vitamin B1, vitamin B2, vitamin E, and vitamin C with equal content. The multivitamin uses conventional commercial products in the field.

[0062] The composite mineral is a mixture of zinc gluconate, ferrous gluconate, calcium lactate, calcium carbonate, sodium iron EDTA, magnesium oxide and calcium hydrogen phosphate with equal content. The composite minerals can be conventional commercial products in the field.

[0063] The preparation method of the above-mentioned selenium-enriched oyster small molecule protein peptide solid beverage includes:

[0064] 1) Crush each item to pass through a 180-mesh sieve and mix according to the formula, and dissolve it in 10 times distilled water;

[0065] 2) Step 1) Incorporate the following mixture into the solution, mix it and let it stand for 12 hours, dry to constant weight and pulverize to the required particle size for solid beverages.

[0066] The preparation method of the solid beverage of the present invention has simple material handling and no loss of nutrients, while retaining multiple active ingredients, which not only improves the utilization rate of raw materials, reduces production costs, and at the same time enables the effective ingredients to be fully absorbed; Sterilizing with the following mixture can quickly kill all kinds of acetic acid bacteria remaining in the beverage, eliminate acetic acid bacteria pollution, inhibit the acidification of acetic acid bacteria on the beverage, and extend the storage of the beverage without using preservatives The deadline is to maintain the high level of color, aroma, taste, and nutrients of the solid beverage after reconstitution.

[0067] The aforementioned mixture is a synergistic mixture containing the following:

[0068] 1) DL-malic acid alkali metal salt and citrate alkaline earth metal salt, and

[0069] 2) Methyl o-hydroxybenzoate, and

[0070] 3) Tyroserval peptide-chlorin e6 metal complex ester represented by formula (1),

[0071] Among them, M in formula (1) is Zn 2+.

[0072] It is used for the sterilization of beverages that have been contaminated by acetic acid bacteria, preferably for their subsequent storage.

[0073] The alkali metal salt of DL-malate used was DL-lithium malate.

[0074] The alkaline earth metal salt of citrate used is magnesium citrate.

[0075] In the preparation method of the selenium-enriched oyster small molecule protein peptide solid beverage, the amounts of the following items incorporated into the beverage are:

[0076] 1) DL-malic acid alkali metal salt 600ppm and citrate alkaline earth metal salt 600ppm, and

[0077] 2) 60 ppm of methyl o-hydroxybenzoate, and

[0078] 3) The casein valerin-chlorin e6 metal complex ester represented by formula (1) is 350 ppm.

[0079] The preparation method of the used casein valerin-chlorin e6 metal complex ester includes the following steps:

[0080] 1) In the presence of light and nitrogen, the metal complex of tyroserval peptide and chlorin e6 undergoes esterification;

[0081] 2) Centrifuge, dialyze the supernatant with deionized water, and freeze-dry;

[0082] 3) The dried product was separated by silica gel column chromatography and eluted with a dichloromethane-acetonitrile gradient eluent;

[0083] 4) After the dichloromethane-acetonitrile is evaporated, it is dissolved in acetonitrile, refined and purified by C-18 reversed phase column chromatography, and eluted with an acetonitrile-water gradient eluent;

[0084] 5) Concentrate the obtained purified liquid to dryness, and then dry the concentrate to obtain.

[0085] The volume ratio of the methylene chloride-acetonitrile gradient eluent used is 1:0, 50:1, 10:1, 1:1, 1:10, 1:50, and 1:0.

[0086] The volume ratio of the acetonitrile-water gradient eluent used was 1:0, 50:1, 10:1, 1:1, 1:10, 1:50, and 1:0.

[0087] The method used for drying the concentrate is freeze-drying to constant weight.

[0088] In the method of the present invention, the above-mentioned method of esterification of caser valetide and chlorin e6 metal complex is performed, followed by dialysis, separation by silica gel column chromatography, purification and purification by reverse phase column chromatography, and finally drying. Tyroservalin-chlorin e6 metal complex ester, the preparation process is easy to control, and the tyroservalin as the polar end that dissolves in water makes the caseervalin-chlorin e6 metal complex ester It is soluble in water and solves the problem of poor water solubility of Ce6. The yield and purity of the final product tyroservalin-chlorin e6 metal complex ester are high, which can greatly improve its combination with DL-malic acid or citric acid and/or its salt and with o-hydroxybenzene Methyl formate synergistically controls the antibacterial effect of acetic acid bacteria and can be used in the disinfection of beverages such as solid beverages.

[0089] Example 2:

[0090] This embodiment provides the following items:

[0091] Item [1]: A synergistic mixture containing the following:

[0092] 1) DL-potassium malate, DL-magnesium malate and potassium citrate, and

[0093] 2) Methyl o-hydroxybenzoate, and

[0094] 3) As shown in formula (1) and M is Ru 2+ Tyroserval peptide-chlorin e6 metal complex ester,

[0095] It is used for the sterilization of beverages that have been contaminated by acetic acid bacteria, preferably for their subsequent storage.

[0096] The preparation method of the used casein valin-chlorin e6 metal complex ester includes the following steps:

[0097] 1) In the presence of light and nitrogen, the metal complex of tyroserval peptide and chlorin e6 undergoes esterification;

[0098] 2) Centrifuge, dialyze the supernatant with deionized water, and freeze-dry;

[0099] 3) The dried product was separated by silica gel column chromatography and eluted with a dichloromethane-acetonitrile gradient eluent;

[0100] 4) After the dichloromethane-acetonitrile is evaporated, it is dissolved in acetonitrile, refined and purified by C-18 reversed phase column chromatography, and eluted with an acetonitrile-water gradient eluent;

[0101] 5) Concentrate the obtained purified liquid to dryness, and then dry the concentrate to obtain.

[0102] The volume ratio of the methylene chloride-acetonitrile gradient eluent used is 1:0, 50:1, 10:1, 1:1, 1:10, 1:50, and 1:0.

[0103] The volume ratio of the acetonitrile-water gradient eluent used was 1:0, 50:1, 10:1, 1:1, 1:10, 1:50, and 1:0.

[0104] The method used for drying the concentrate is vacuum drying to constant weight.

[0105] In the method of the present invention, the above-mentioned method of esterification of caser valetide and chlorin e6 metal complex is performed, followed by dialysis, separation by silica gel column chromatography, purification and purification by reverse phase column chromatography, and finally drying. Tyroservalin-chlorin e6 metal complex ester, the preparation process is easy to control, and the tyroservalin as the polar end that dissolves in water makes the caseervalin-chlorin e6 metal complex ester It is soluble in water and solves the problem of poor water solubility of Ce6. The yield and purity of the final product tyroservalin-chlorin e6 metal complex ester are high, which can greatly improve its combination with DL-malic acid or citric acid and/or its salt and with o-hydroxybenzene Methyl formate synergistically controls the antibacterial effect of acetic acid bacteria and can be used in the disinfection of beverages such as solid beverages.

[0106] Item [2]: Use of the mixture of Item [1], which includes the use of the mixture of Item [1] for the sterilization of beverages that have been contaminated by acetic acid bacteria.

[0107] The use of the mixture described in Item [1] also includes the use of the mixture in Item [1] for subsequent preservation of beverages.

[0108] In general, based on the amount of the beverage to be sterilized, the incorporation amount of DL-potassium malate is 300 ppm, the incorporation amount of DL-magnesium malate is 300 ppm, and the incorporation amount of potassium citrate is 300 ppm, The amount of methyl hydroxybenzoate incorporated is 70 ppm, as shown in formula (1) and M is Ru 2+ The amount of Tyroservalin-Chlorin e6 metal complex ester is 375ppm.

[0109] According to the present invention, the contamination of acetic acid bacteria should be understood to mean the detection of a significant level of acetic acid bacteria in beverages caused by detection methods known from the prior art to those skilled in the art. The detection of bacteria according to these methods is already a pollution in the context of the present invention and needs to be eliminated. The method item [1] of the present invention includes 1) DL-malic acid and/or its alkali metal salt and/or its alkaline earth metal salt or citric acid and/or its alkali metal salt and/or its alkaline earth metal salt, and 2 ) Methyl o-hydroxybenzoate, and 3) At least one type of casein-chlorin e6 metal complex ester represented by formula (1) has a synergistic effect. For the sterilization of beverages that have been contaminated by acetic acid bacteria and/or for the subsequent preservation of beverages, the synergy index SI in the combined application of the above 1), 2) and 3) <1, can effectively play a synergistic effect, quickly inhibit and kill various acetic acid bacteria in the beverage, eliminate acetic acid bacteria pollution, inhibit the acidification of acetic acid bacteria on the beverage, and extend the beverage without using preservatives The shelf life of the beverage or solid beverage is maintained at a high level of color, aroma, taste, and nutrients.

[0110] Item [3]: Since the purpose of the present invention also involves incorporating the above mixture into a beverage to be sterilized, the present invention also relates to a method for sterilizing beverages that have been contaminated with acetic acid bacteria, including [1] or [2] is incorporated into the beverage.

[0111] This combination can be carried out, for example, by adding the individual items a), b) and c) of the mixture separately in any order or by adding the aforementioned mixture as a total mixture. This mixture can be diluted by additional solvents or also by other additives (such as emulsifiers) or other suitable food additives. As the solvent, hydrophilic, organic and water-miscible solvents can be used. Preferably, no additional solvent is added. These compounds are preferably incorporated into the beverage separately.

[0112] Item [4]: ​​The mixture used according to the present invention exerts the effect of resisting acetic acid bacteria in a synergistic manner. The present invention also relates to a method for preserving sterilized beverages, including adding the following items [1] or [2] ] The mixture is incorporated into the beverage.

[0113] Item [5]: According to the purpose of the present invention, the present invention also relates to a selenium-enriched oyster small molecule protein peptide solid beverage, which includes the following items in parts by weight: 75 parts by weight of selenium-enriched oyster small molecule protein peptide , 25 parts by weight of selenium-rich cooked wheat flour, 2 parts by weight of spirulina powder, 8 parts by weight of konjac flour, 20 parts by weight of oligofructose, 10 parts by weight of vegetable protein, 8 parts by weight of cooked quinoa flour, 20 parts by weight of skimmed milk powder, compound mineral 2 parts by weight of the substance and 2 parts by weight of the multivitamin, and the solid beverage is sterilized by the mixture according to any one of items [1] to [4]. In the present invention, the selenium-enriched oyster small molecule protein peptide can quickly supplement the nutrients needed in the body, and can hardly consume energy in the body to reach the required nutritional position. The source is not particularly limited, and conventional commercial products in the field can be used, or they can be prepared from selenium-enriched oysters through cleaning, crushing, cooking, enzymatic hydrolysis, filtration, concentration, and drying. The solid beverage of the present invention uses selenium-enriched oyster small molecule protein peptides and selenium-enriched cooked wheat flour as the main raw materials, and is mixed with spirulina powder, konjac flour, oligofructose, vegetable protein, cooked quinoa flour, skimmed milk powder, complex minerals and Compound vitamins and other ingredients make the solid beverage of the present invention have a comprehensive, balanced and reasonable nutrient composition, good satiety, good effect, quick effect, ready-to-eat preparation, good for body absorption, good for helping people to shape their bodies, and good To meet people's requirements for a perfect body, the solid beverage of the present invention is rich in protein, vitamins and selenium, and has high nutritional value.

[0114] The multivitamin is a mixture of vitamin A, vitamin D, vitamin B1, vitamin B2, vitamin B6, niacin, vitamin E, pantothenic acid and vitamin C in equal content. In the present invention, the sources of the vitamin A, vitamin D, vitamin B1, vitamin B2, vitamin B6, niacin, vitamin E, pantothenic acid and vitamin C are not particularly limited, and conventional commercial products in the field can be used.

[0115] As a preferred embodiment of the present invention, the composite mineral is a mixture of zinc gluconate, ferrous gluconate, calcium lactate, calcium carbonate, sodium iron EDTA, magnesium oxide and calcium hydrogen phosphate in equal content. The present invention does not specifically limit the sources of the zinc gluconate, ferrous gluconate, calcium lactate, calcium carbonate, sodium iron EDTA, magnesium oxide and calcium hydrogen phosphate, and conventional commercial products in the field can be used.

[0116] Item [6]: According to the purpose of the present invention, the present invention also relates to the preparation method of the selenium-enriched oyster small molecule protein peptide solid beverage of item [5], which includes:

[0117] 1) Crush the items in item [5] to pass through a 200-mesh sieve and mix according to the formula, and dissolve in distilled water;

[0118] 2) Step 1) Add the mixture described in any one of items [1] to [4] to the solution, mix it and let it stand for 24 hours, dry to constant weight and pulverize to the required particle size for solid beverages.

[0119] The preparation method of the solid beverage of the present invention has simple material handling and no loss of nutrients, while retaining multiple active ingredients, which not only improves the utilization rate of raw materials, reduces production costs, and at the same time enables the effective ingredients to be fully absorbed; The sterilization treatment of the mixture described in any one of items [1] to [4] can quickly kill various acetic acid bacteria remaining in the beverage, eliminate acetic acid bacteria pollution, inhibit the acidification effect of acetic acid bacteria on beverages, and Extend the shelf life of the beverage without using preservatives, and maintain the high level of color, aroma, taste, and nutrients of the solid beverage after reconstitution.

[0120] Example 3:

[0121] Example 3 is basically the same as Example 2, except that in Example 3, the synergistic mixture is as follows:

[0122] 1) DL-Sodium Malate 300ppm and Lithium Citrate 300ppm, and

[0123] 2) 70 ppm of methyl o-hydroxybenzoate, and

[0124] 3) As shown in formula (1) and M is Ca 2+ The casein valetin-chlorin e6 metal complex ester of 375 ppm, which is used for the sterilization of beverages that have been contaminated by acetic acid bacteria, and is preferably used for its subsequent storage.