Preparation method of universal short tandem repeat (STR) allele ladder

A technology of short tandem repeats and alleles, applied in the field of allelic ladder preparation, can solve the problems of large workload and cost, high cost of whole gene synthesis, deviation of allele yield, etc., and achieves saving cycle and cost, saving Workload and production cost, the effect of saving labor cost and time cost
CN110229871AActive Publication Date: 2019-09-13上海晶准生物医药有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
上海晶准生物医药有限公司
Publication Date
2019-09-13

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Abstract

The invention provides a preparation method of a universal short tandem repeat (STR) allele ladder. The preparation method comprises the following steps of (1) designing and synthesizing a primer anda plasmid according to an STR locus; (2) using the primer to perform a PCR amplification reaction by using the plasmid as an amplification formwork; and (3) performing purification on PCR amplification products, and performing appropriate dilution so as to obtain the STR allele ladder. According to the preparation method disclosed by the invention, only one plasmid needs to be synthesized, the PCRreaction needs to be performed only once, and after the PCR products are subjected to purification and dilution, the allele ladder of the corresponding STR locus can be obtained, so that the preparation technological process of the allele ladder of the STR locus is greatly simplified, and labor cost,, time cost and economic cost for preparation are saved.
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Description

technical field

[0001] The invention relates to the technical field of genetic engineering, in particular to a method for preparing a general short tandem repeat (STR) allele ladder (Allele Ladder) and a kit thereof. Background technique

[0002] A genetic marker called short tandem repeat (STR) exists in human and various genomes. STR is widely used in the research of genetics, forensic science, oncology, etc., especially those STR genetic markers with high polymorphism in the population. Such as Dib C and his colleagues in 1996 (Nature.1996 Mar 14; 380(6570):152-4.PMID:8600387), Broman KW is equal to 1998 (Am J Hum Genet.1998 Sep; 63(3):861- 9.PMID:9718341), Kong A et al. in 2002 (Nat Genet.2002 Jul; 31(3):241-7.PMID:12053178) used 5236, 8325, and 5136 STR genetic markers respectively for high-resolution Genetic mapping of the human genome. In the field of forensic science, STR markers have become standard genetic markers for individual identification and parentage dete...

Claims

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