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Alpha-glucosidase inhibitor extracted from Potentilla bifurca var. Humilior R

A technology of Potentilla biscuits and glucosidase, which is applied in the field of traditional Chinese medicine and can solve the problems of high price of acarbose, increased gas production, and side effects

Active Publication Date: 2019-09-20
QINGHAI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are problems in the existing technology: acarbose, the most commonly used α-glucosidase inhibitor hypoglycemic drug, can effectively control diabetes and lower blood sugar, but its applicable population is limited, and it is not suitable for high-protein foods such as meat and eggs. People who eat high-fat food as their staple food, at the same time, because of their slow decomposition and absorption in the small intestine, prolonged residence time, and increased gas production through the fermentation of intestinal bacteria, it can cause abdominal distension, abdominal pain and diarrhea, etc., and the content of acarbose Higher price, not suitable for lower income groups
Other hypoglycemic drugs also have different degrees of side effects and social problems

Method used

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  • Alpha-glucosidase inhibitor extracted from Potentilla bifurca var. Humilior R
  • Alpha-glucosidase inhibitor extracted from Potentilla bifurca var. Humilior R
  • Alpha-glucosidase inhibitor extracted from Potentilla bifurca var. Humilior R

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Embodiment 1

[0030]This embodiment provides a kind of α-glucosidase inhibitor extracted from Potentilla biscuit dwarf, described inhibitor is 1-O-coumaroyl-β-D-glucose (1-O-coumaroyl- β-D-glucose), its chemical structure is attached figure 2 shown. The α-glucosidase inhibitor adopts the assay method of α-glucosidase in vitro activity to measure the enzyme inhibitory activity. The results show that: when the sample concentration is 2.5mg / mL, 1-O-coumaroyl-β-D - Glucose (1-O-coumaroyl-β-D-glucose) α-glucosidase inhibition rate was 99.41%, IC 50 It is 0.0019mg / mL.

Embodiment 2

[0032] This embodiment provides an α-glucosidase inhibitor extracted from Potentilla bischiifolia, said inhibitor is: myricetin, and the α-glucosidase inhibitor uses α-glucoside The enzyme in vitro activity assay method was used to measure the enzyme inhibitory activity. The results showed that: when the sample concentration was 1.25 mg / mL, the α-glucosidase inhibition rate of myricetin (myricetin) was 90.67%, IC 50 It is 0.0561mg / mL, and its chemical structure is as attached image 3 shown.

Embodiment 3

[0034] This embodiment provides an α-glucosidase inhibitor extracted from Potentilla bifida dwarf, the inhibitor is: tiliroside, the α-glucosidase inhibitor uses α-glucose The assay method of in vitro activity of glucosidase was carried out to measure the enzyme inhibitory activity. The results showed that: when the sample concentration was 1.25 mg / mL, the α-glucosidase inhibition rate of tiliroside (tiliroside) was 87.38%, IC 50 It is 0.1807mg / mL, and its chemical structure is as attached Figure 4 shown.

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Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to an alpha-glucosidase inhibitor extracted from Potentilla bifurca var. Humilior R. Extraction, column chromatography isolation and glycosidase inhibitory activity verification are performed on the Potentilla bifurca var. Humilior R to obtain the alpha-glucosidase inhibitor. The alpha-glucosidase inhibitor is extracted from natural plant Potentilla bifurca var. Humilior R widely distributed on Qinghai-Tibet Plateau and serves as a monomeric compound, the alpha-glucosidase inhibitor is high in purity and inhibition ratio, an extraction process is simple, convenient and safe, alpha-glucosidase inhibitory activity of the alpha-glucosidase inhibitor is firstly discovered, inhibitory effect is good, a basis can be provided for preparation of novel hypoglycemic drugs, and the alpha-glucosidase inhibitor can serve as a drug for reducing postprandial blood sugar of diabetic patients.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to an α-glucosidase inhibitor extracted from Potentilla biscuits and its extraction method and application. Background technique [0002] Diabetes is one of the most serious world health diseases in the 21st century, which has brought great harm to human beings. α-Glucosidase inhibitors are currently commonly used hypoglycemic drugs, but the synthetic drugs have many side effects and cause varying degrees of damage to the human body. The hypoglycemic active ingredients extracted and separated from natural plants have low toxicity and have good hypoglycemic effect at the same time. The extraction and separation of hypoglycemic active ingredients from natural plants is helpful for the development of new drugs and the development and utilization of natural plants. [0003] Dwarf bifid Potentilla ( Potentilla bifurca var. Humilior R) is a family of Rosaceae ( ...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08C07H17/07C07H13/08C07D311/30C07D311/40C07D311/62A61P3/10A61K31/352A61K31/353A61K31/7024A61K31/7048
CPCC07H15/18C07H1/08C07H17/07C07H13/08C07D311/30C07D311/40C07D311/62A61P3/10
Inventor 曾阳严培瑛李锦萍刘力宽龙主多杰李彩明曲宣诏唐勋
Owner QINGHAI NORMAL UNIV
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