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Talaromyces verruculosus SBS-ZGTP02 and application thereof

A sycamore pine and verrucous basket-like technology is applied to the rhizosphere Talaromyces verruca SBS-ZGTP02 and its application fields, and can solve the problems of pathogenic bacteria soil microecological structure destruction, environmental pollution, poor seedling growth and the like

Active Publication Date: 2019-09-20
HEILONGJIANG PROV FOREST PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pinus sylvestris is the main afforestation tree species in northern my country. It has the characteristics of strong stress resistance and rapid growth. It plays an important role in ecological restoration, landscaping, windbreak and sand fixation. At present, there are too many pesticides used in the production of sylvestris sylvestris. Pathogens produce drug resistance, soil micro-ecological structure damage, environmental pollution, and poor seedling growth. The use of probiotics can play an important role in promoting seedling growth, improving seedling disease resistance, and improving rhizosphere micro-ecological environment. Phosphorus fungi belong to plant probiotics, but there is no report about phosphorus-dissolving fungi adapted to the rhizosphere of Pinus sylvestris sylvestris in the prior art

Method used

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  • Talaromyces verruculosus SBS-ZGTP02 and application thereof
  • Talaromyces verruculosus SBS-ZGTP02 and application thereof
  • Talaromyces verruculosus SBS-ZGTP02 and application thereof

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Experimental program
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Effect test

Embodiment 1

[0031] Phosphorus Solubilization of Talaromyces verruculosus SBS-ZGTP02

[0032] 1) Phospholytic circle of strains in solid plate conditions

[0033] Inoculate Talaromyces verruculosus SBS-ZGTP02 on an NBRIP plate and culture at 25°C. After 7 days, observe whether there is a transparent circle, measure the diameter of the colony (D) and transparent circle (d), and calculate the transparent circle The ratio of the diameter to the colony diameter (d / D) can preliminarily estimate the phosphorus-solubilizing ability of the phosphorus-solubilizing fungus. The larger the ratio, the stronger the phosphorus-solubilizing ability of the fungus. The d / D value of T. verruculatus was measured by the above method, and the result was that the measured ratio was 1.55, which indicated that the T. verrucosus strain had a strong phosphorus-solubilizing ability. see results figure 1 .

[0034] 2) Phosphorus solubilization of insoluble inorganic phosphate by strains under liquid fermentation c...

Embodiment 2

[0046] Growth promotion of Talaromyces verruculosus SBS-ZGTP02 on Pinus sylvestris sylvestris seedlings

[0047] Preparation of solid microbial agent: After bacterial strain SBS-ZGTP02 is activated on PDA medium for 5 days, use a sterile puncher (10mm in diameter) to remove 2 pieces of bacterial cakes and inoculate them in a triangular flask (500mL) filled with 250mLPD liquid medium. Place it on a shaker (25°C, 150r / min) and vibrate for 7 days, inoculate the cultured liquid strains on the solid medium of cottonseed hulls, and culture them statically at 25°C in the dark for 15 days. Take out the culture medium and prepare it as a solid bacterial agent for future use. The spore content of the bacterial agent is 4.0×10 8 spores / g bacterial agent.

[0048] Three treatments were inoculated: 1) solid bacterial agent of T. verrucous SBS-ZGTP02; 2) blank control of cottonseed hull solid medium; 3) blank control of sterile water.

[0049] After the germination of Pinus sylvestris is ...

Embodiment 3

[0056] Improvement of physical and chemical properties of rhizosphere soil of Pinus sylvestris sylvestris by Talaromyces verruculosus SBS-ZGTP02

[0057] After 90 days of the emergence of Pinus sylvestris seedlings in embodiment 2, each process randomly collects 70 Pinus sylvestris seedlings and shakes the rhizosphere gently and collects the rhizosphere soil samples with a sterile sample bag, and puts them into a container with a 2mm sieve after sieving. Bring it back to the laboratory in an incubator with ice packs, and store it in a refrigerator at 4°C after natural air drying. Soil organic matter was measured by potassium dichromate external heating method, total nitrogen was measured by Kjeldahl nitrogen analyzer, total phosphorus was measured by molybdenum antimony anti-colorimetric method, and available phosphorus was measured by double-acid extraction molybdenum antimony anti-colorimetric method. Available Potassium NH 4 OAc was extracted by flame photometer method, to...

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Abstract

The invention provides a talaromyces verruculosus SBS-ZGTP02 and an application thereof, and belongs to the technical field of microorganisms. The talaromyces verruculosus is Latin words, and the preservation number is CGMCC No. 17768. The talaromyces verruculosus can improve the dissolution of insoluble phosphate, promote the growth of pinus sylvestris seedlings, improve the rhizosphere soil and physical and chemical properties of pinus sylvestris, and improve the enzyme activity in the rhizosphere soil of pinus sylvestris.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain SBS-ZGTP02 of pine rhizosphere verrucous bacteria SBS-ZGTP02 and its application. Background technique [0002] Phosphorus is one of the three essential nutrients for plant growth. Under natural conditions, the available phosphorus available to plants only accounts for a small part of the total phosphorus content. Phosphorus-solubilizing microorganisms refer to the ability to convert insoluble phosphorus into soluble phosphorus that plants can absorb and utilize in the soil, thereby improving the absorption efficiency of plants to rhizosphere phosphorus, promoting plant growth, and improving their stress resistance. Phosphorus-dissolving microorganisms include bacteria, fungi, and actinomycetes. Because bacteria and actinomycetes have the phenomenon of degraded phosphorus-dissolving efficiency in subculture, and phosphorus-dissolving fungi are relatively sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C09K17/14C12R1/645
CPCC09K17/14C12R2001/645C12N1/145A01N63/30
Inventor 邓勋宋小双遇文婧闵凯周琦刘欣
Owner HEILONGJIANG PROV FOREST PROTECTION INST
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