Application of astaxanthin to preparation of aldose reductase inhibitor

A reductase inhibitor, astaxanthin technology, applied in the field of application of astaxanthin in the preparation of aldose reductase inhibitors, to achieve the effect of reducing the occurrence of diabetic complications

Pending Publication Date: 2019-10-08
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Astaxanthin is a ubiquitous terpene non-V A The source of carotenoids has biological activities such as delaying aging, inhibiting tumors, enhancing immunity, protecting nerves, re

Method used

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  • Application of astaxanthin to preparation of aldose reductase inhibitor
  • Application of astaxanthin to preparation of aldose reductase inhibitor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The preparation of embodiment 1 astaxanthin

[0029] The Phaffia fermented liquid was 5534×g, centrifuged for 8 min, the supernatant was discarded, washed with distilled water and centrifuged again, and the operation was repeated twice to obtain Phaffia cells.

[0030] Phaffia yeast cell wall breaking: Use dimethyl sulfoxide to break the Phaffia yeast cell wall, the breaking condition is: the cell and dimethyl sulfoxide are based on the mass-volume ratio, that is, g / ml, and the ratio is 1 / 2. At 55°C, break the wall for 5 minutes; using dimethyl sulfoxide has a high breaking rate and good extraction effect; the short treatment time (5min) and low treatment temperature (55°C) are conducive to the stability of the astaxanthin structure .

[0031] Crude extraction of astaxanthin with acetone: after breaking the wall, extract with acetone according to the ratio of material to liquid 1:15, the ratio of material to liquid is the mass-volume ratio of Phaffia thallus to aceton...

Embodiment 2

[0036] The measurement of the inhibitory rate of embodiment 2 astaxanthin to aldose reductase activity

[0037] Preparation of reagents:

[0038] Preparation of phosphate buffered saline (PBS): accurately weigh 6.36g NaH 2 PO 4 , add distilled water to dissolve, transfer to a 250mL volumetric flask to obtain liquid a; take another 3.31g Na 2 HPO 4 , add distilled water to dissolve, transfer to a 250mL volumetric flask to constant volume to obtain liquid b; then, pipette the two solutions of a and b to a 500mL volumetric flask to constant volume, use a pH meter to calibrate the phosphate buffer solution to pH=6.2 .

[0039] Preparation of aldose reductase solution: Take 100 U of aldose reductase powder and add 1 mL of PBS, that is, the enzyme activity of aldose reductase is 100 U / mL. Take 20 μL of 100 U / mL aldose reductase solution and add 980 μL of PBS, that is, the concentration of aldose reductase is 2 U / mL, and the aldose reductase solution should be prepared and used im...

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Abstract

The invention discloses new application of astaxanthin, and particularly relates to application of the astaxanthin to preparation of an aldose reductase inhibitor. According to application, the astaxanthin serves as one of active components or the only active component for preparing the aldose reductase inhibitor. The inventor finds that the astaxanthin has a significant inhibitory effect on aldose reductase, the aldose reductase is a key rate-limiting enzyme of a polyol pathway, activation of the polyol pathway is an important reason for occurrence and development of diabetic microvascular complications, the astaxanthin can inhibit activation of the polyol pathway by inhibiting the activity of the aldose reductase, and then generation of the diabetic complications is reduced; and the astaxanthin does not generate side effects, can be applied to preparation of drugs for treatment or prevention of the diabetic complications, and can also be applied to preparation of health care productsfor adjuvant treatment of the diabetic complications.

Description

technical field [0001] The invention relates to a new application of astaxanthin, in particular to an application of astaxanthin in preparing an aldose reductase inhibitor. Background technique [0002] Diabetic complications, such as cataracts, demyelinating diseases of the nervous system, and edema of nerves and surrounding tissues, are caused by the accumulation of sorbitol in the body and increased osmotic pressure due to abnormal polyol metabolism pathways in diabetic patients. Aldose reductase (A1dose Reductase, AR) is a key enzyme in the metabolic pathway of polyols. The high blood sugar concentration of diabetic patients is usually the key reason for the activation of aldose reductase. After it is activated, it will reduce glucose into sorbitol, sorbitol And because of its polarity, it is difficult to pass through the cell membrane, causing accumulation in the body, increasing the osmotic pressure in the cell, causing cell damage, leading to glucose metabolism disord...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P3/10C07C403/24A23L33/145
CPCA61K31/122A61P3/10C07C403/24A23L33/145C07C2601/16A23V2002/00A23V2200/328A23V2250/218
Inventor 杜希萍王鑫倪辉姜泽东黄高凌杨远帆李利君陈艳红朱艳冰肖安风张永辉李志朋
Owner JIMEI UNIV
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