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Effective part of dendrobium cymbium alkaloid and preparation method and application thereof

A technology of Dendrobium officinale and effective parts is applied to the effective parts of Dendrobium officinale and its preparation field, which can solve problems such as insufficient work, and achieve the effects of improving hyperlipidemia and lowering blood sugar.

Active Publication Date: 2019-10-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, insufficient work has been done on the screening and determination of the "effectiveness" of active parts

Method used

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  • Effective part of dendrobium cymbium alkaloid and preparation method and application thereof
  • Effective part of dendrobium cymbium alkaloid and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Preparation of effective fractions of Dendrobium cyclamen alkaloids

[0052] S1. Cold-soak 1 kg of Dendrobium cinnamon with 80% methanol aqueous solution at room temperature, the ratio of solid to liquid is 8:1, ultrasonically assisted extraction, 80min / d, place for 48h, filter, concentrate the filtrate to obtain the extract of Dendrobium cinnabar; repeat the extraction 3 times.

[0053]S2. The Dendrobium cyclamen extract obtained in S1 is dissolved in an appropriate amount of methanol, and subjected to solid-phase extraction on a C-18 reverse-phase silica gel column. The ratio is 0.4kg: 1L. After loading the sample, elute with 6 times the bed volume of water to remove the fat-soluble part, collect the water eluate, concentrate and dry to obtain the effective part of the crude dendrobium alkaloid.

[0054] S3. Add appropriate amount of methanol to dissolve the effective part of the crude Dendrobium alkaloids obtained in S2, put it on a Sephadex LH-20 column, ...

Embodiment 2

[0055] Example 2 Preparation of effective fractions of Dendrobium cyclamen alkaloids

[0056] S1. Cold-soak 1kg of Dendrobium cyclamen with 80% ethanol aqueous solution at room temperature, the ratio of solid to liquid is 10:1, ultrasonically assisted extraction, 80min / d, place for 48h, filter, concentrate the filtrate to obtain the extract of Dendrobium cyclamen; repeat the extraction 3 times.

[0057] S2. With the Dendrobium cyclamen extract obtained in S1, add an appropriate amount of water to dissolve, and perform solid-phase extraction on a non-polar macroporous adsorption resin column. The ratio is 0.4kg: 1L. After loading the sample, elute with 6 times the bed volume of water, separate the fat-soluble part, collect the water eluate, concentrate and dry to obtain the effective part of the crude dendrobium alkaloid.

[0058] S3. Add appropriate amount of methanol to dissolve the effective part of the crude Dendrobium alkaloids obtained in S2, put it on a Sephadex LH-20 c...

Embodiment 3

[0059] Example 3 Determination of Dendrobium Ning content in the active parts of Herba Dendrobii alkaloids

[0060] The content analysis of Dendrobium Ning in the effective part of the alkaloids of Dendrobium candidum adopts the quantitative analysis technology of hydrogen nuclear magnetic resonance.

[0061] 1. Specific methods

[0062] Specifically, a 500MHz nuclear magnetic resonance instrument is used, and the experimental parameters are set as follows: pulse sequence zg30, spectral width SW=14 ppm, center frequency O1P=6 ppm, sampling time AQ=3 s, number of sampling points TD=41976, waiting time D1=2 s, The number of scans NS=48, the number of conversion points SI=65536, the quantitative peak selects the g-position CH proton peak in the Dendrobium structure. The proton on the aromatic ring in the salicylic acid molecule was used as the external standard, and quantified by the -ERETIC2 method.

[0063] Sample operation: Accurately weigh 10 mg of the active part extract o...

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Abstract

The invention discloses an effective part of dendrobium cymbium alkaloid and a preparation method and an application thereof. The effective part of the dendrobium cymbium alkaloid contains the alkaloid shihunine. The invention provides an effective part of the dendrobium cymbium alkaloid, which has a new composition, and the effective part clears the active ingredients, which is beneficial to thequality control of the medicine and the safety of the medicine. The animal in-vivo activity study shows that the effective part of the dendrobium cymbium alkaloid can significantly reduce the blood glucose of the db / db diabetic mice, improves the hyperlipidemia state of the type 2 diabetic mice, and has the application prospect for development of drugs or health products for the treatment of typeII diabetes.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, and more specifically, relates to an active part of the alkaloids of Dendrobium officinalis and its preparation method and application. Background technique [0002] Traditional Chinese medicine has a long history of thousands of years in treating diabetes in my country, and has the advantage of less toxicity than Western medicine. Dendrobium is commonly used in traditional Chinese medicine to treat fever, fluid injury, dry mouth and polydipsia, insufficient stomach yin, lack of food and retching, persistent deficiency of heat after illness, hyperactivity of fire due to deficiency of yin, bone steaming, fatigue and heat, blurred vision, and flaccidity of muscles and bones embolism. It has been reported that co-decoction of Dendrobium and multi-flavored traditional Chinese medicine has definite curative effect on reducing blood sugar, blood lipid and insulin resistance in pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8984A61K31/407A61P3/10A61P3/06A23L33/105
CPCA23V2002/00A61K31/407A61K36/8984A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23L33/105A61P3/06A61P3/10A23V2200/30A23V2200/328A23V2250/21
Inventor 王军李雪文刘岚陈慧萍
Owner SUN YAT SEN UNIV