Microfine soybean fiber encapsulation and embedding method for improving gastrointestinal fluid tolerance of lactic acid bacteria
A technology of soybean fiber and soybean cellulose, which is applied in the field of food biology, can solve the problems of water-soluble wall material gel ability decline, mechanical strength weakening, etc., to meet the needs of high-activity lactic acid bacteria food, strong operational applicability, and suitable processing strong effect
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Embodiment 1
[0038] This embodiment provides a micronized soybean fiber encapsulation embedding technology that improves the tolerance of lactic acid bacteria to gastrointestinal fluids. The specific steps are:
[0039] (1) Soybean cellulose preparation: weigh 100g bean dregs and anhydrous ethanol in a mass ratio of 1:10, mix them in a water bath at 50°C for 6 hours, degrease, filter, change the ethanol every 2 hours, dry the defatted bean dregs, and then pulverize them. Bean dregs fine powder. Weigh 50g of bean dregs fine powder and 5% NaOH solution and mix them at a mass ratio of 1:25. Stir in a water bath at 50°C for 4 hours to remove the lignin in the bean dregs. After centrifugation, add water to the precipitate and dilute it back to its original volume. Adjust the pH to 9 and then add Alkaline protease (200000U / g) of 0.5wt% dry weight of bean dregs, stirred at 40°C for 4 hours, suction filtered, washed with water, and then suction filtered to obtain soybean cellulose.
[0040] (2) M...
Embodiment 2
[0043] This embodiment provides a micronized soybean fiber encapsulation embedding technology that improves the tolerance of lactic acid bacteria to gastrointestinal fluids. The specific steps are:
[0044] (1) Soybean cellulose preparation: weigh 100g bean dregs and anhydrous ethanol and mix them in a mass ratio of 1:10, heat and stir in a water bath at 50°C for 6 hours to degrease, then filter, change the ethanol every 2 hours, and dry the defatted bean dregs before crushing Obtain fine powder of bean dregs. Weigh 50g of bean dregs fine powder and 5% NaOH solution and mix them at a mass ratio of 1:25. Stir in a water bath at 50°C for 4 hours to remove the lignin in the bean dregs. After centrifugation, add water to the precipitate and dilute it back to its original volume. Adjust the pH to 9 and then add Alkaline protease (200000U / g) with 0.5% dry weight of bean dregs, stirred at 40°C for 4 hours, suction filtered, washed with water, and then suction filtered to obtain soybe...
Embodiment 3
[0048] This embodiment provides a micronized soybean fiber encapsulation embedding technology that improves the tolerance of lactic acid bacteria to gastrointestinal fluids. The specific steps are:
[0049] (1) Soybean cellulose preparation: weigh 100g bean dregs and anhydrous ethanol in a mass ratio of 1:10, mix them in a water bath at 50°C for 6 hours, degrease, filter, change the ethanol every 2 hours, dry the defatted bean dregs, and then pulverize them. Bean dregs fine powder. Weigh 50g of bean dregs fine powder and 5% NaOH solution and mix them at a mass ratio of 1:25. Stir in a water bath at 50°C for 4 hours to remove the lignin in the bean dregs. After centrifugation, add water to the precipitate and dilute it back to its original volume. Adjust the pH to 9 and then add Dry soybean dregs with 0.5% alkaline protease (200000U / g), stir and enzymolyze at 40°C for 4 hours, filter with suction, wash with water, and then filter with suction to obtain soybean cellulose.
[00...
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