Chitosan hydrogel, and preparation method and application thereof

A technology of chitosan and thiolated chitosan, which is applied in the direction of additive processing, can solve the problems of reduced printability and application range, low mechanical strength and toughness of the colloid, and slow curing speed, and can achieve biodegradation speed. Adjustable, adjustable mechanical strength, fast curing effect

Active Publication Date: 2019-10-18
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most 3D bioprinting hydrogels have problems such as slow curing speed, low mechanical strength and toughness of the colloid or cannot be adjusted, which greatly reduces their printability and application range.

Method used

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  • Chitosan hydrogel, and preparation method and application thereof
  • Chitosan hydrogel, and preparation method and application thereof
  • Chitosan hydrogel, and preparation method and application thereof

Examples

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preparation example Construction

[0022] A preparation method of chitosan hydrogel, comprising: carrying out Michael addition reaction of α-β unsaturated acylated chitosan and thiolated chitosan; the general formula of α-β unsaturated acylated chitosan for: The general formula of thiolated chitosan is:

[0023] Among them, R 1 Remove the residues of amino groups for chitosan macromolecules; R 2 is a hydrogen atom, an alkyl group or an alkylene group; R 3 is carbonyl, carboxyl, ester, amide, alkyl or substituted alkyl; R 4 is an alkylene or substituted alkylene.

[0024] Chitosan (CS), also known as chitosan, is the product of deacetylation of chitin and is a widely used natural polysaccharide. Chitosan is the only alkaline polysaccharide in nature. The free amino nitrogen atom on its molecular chain has a pair of unshared electron pairs, which can combine with a hydrogen proton, so that chitosan becomes a positively charged polyelectrolyte. Chitosan has good biocompatibility, biodegradability, bacteri...

Embodiment 1

[0073] The preparation method of the chitosan hydrogel provided by the present embodiment comprises:

[0074](1) Accurately weigh 1.5 g of chitosan and dissolve it in acetic acid with a mass fraction of 0.01%. After being substantially uniformly dissolved under the action of a magnetic stirrer, place it in an ultrasonic oscillator for 30 minutes of sonication. Accurately weigh 1.8 g of the acylating reagent (maleic anhydride), add 5 ml of acetone to dissolve it completely. Add the dissolved acylation reagent into the chitosan acetic acid solution at a uniform speed and slowly, and stir for about 20 minutes under a magnetic stirrer to make it fully mixed. Transfer the uniformly mixed liquid into a 150ml round-bottomed flask, place it in a heat-collecting magnetic stirrer, set the temperature at 40°C, and heat at a constant temperature for 2 hours. Dialysis was performed for three days after the reaction stopped, and the dialysate was changed every 5 hours. Freeze-drying for ...

Embodiment 2

[0080] The preparation method of the chitosan hydrogel provided by the present embodiment comprises:

[0081] (1) Accurately weigh 1.5 g of chitosan and dissolve it in acetic acid with a mass fraction of 0.01%. After being substantially uniformly dissolved under the action of a magnetic stirrer, place it in an ultrasonic oscillator for 30 minutes of sonication. Accurately weigh 1.8 g of the acylating agent (β-methacrylic acid), add 5 ml of acetone to dissolve it completely. Add the dissolved acylation reagent into the chitosan acetic acid solution at a uniform speed and slowly, and stir for about 20 minutes under a magnetic stirrer to make it fully mixed. Transfer the uniformly mixed liquid into a 150ml round-bottomed flask, place it in a heat-collecting magnetic stirrer, set the temperature at 40°C, and heat at a constant temperature for 2 hours. Dialysis was performed for three days after the reaction stopped, and the dialysate was changed every 5 hours. Freeze-drying for...

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Abstract

The invention relates to the technical field of biological materials, and particularly relates to chitosan hydrogel and a preparation method thereof. Alpha-beta unsaturated structures are separately grafted on molecular chains of chitosan; thiol groups are grafted on the molecular chains of the chitosan; the twice modified chitosan is subjected to an Michael addition reaction; rapid curing of chitosan hydrogel is realized by using a chemical crosslinking mechanism, so that the 3D printing property of the material is realized; and mechanical strength of the chitosan hydrogel is adjusted by changing the concentration and the grafting ratio of the modified material. The chitosan hydrogel is high in curing speed, good in biocompatibility, adjustable in mechanical strength, good in stability and adjustable in biodegradation speed. The general formula of the chitosan hydrogel is selected from the following two formulae, wherein R<1> is a residue part obtained after amino is removed from chitosan; R<2> is a hydrogen atom, alkyl or alkylene; R<3> is carbonyl, carboxyl, an ester group, amide, alkyl or substituted alkyl; and R<4> is alkylene or substituted alkylene.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to a chitosan hydrogel and a preparation method and application thereof. Background technique [0002] 3D printing, also known as additive manufacturing, has revolutionized many fields, such as engineering, manufacturing industry, education, and medicine. 3D bioprinting makes it possible to assemble biocompatible materials, cells, and auxiliary components into three-dimensional functionally active human organs or tissues. Compared with non-bioprinting, 3D bioprinting is more complex, involving the selection of biocompatible materials, consideration of cell types, growth, differentiation factors, and tissue construction, among which the selection of materials is crucial. [0003] Hydrogel is a kind of three-dimensional network polymer with hydrophilic groups, which can absorb a large amount of water and swell but is insoluble in water. Because of its composition and structure s...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L5/08B33Y70/00
CPCB33Y70/00C08J3/075C08J2305/08
Inventor 戴建武陈艳艳储筠黄雷
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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