Temperature/pH responsive double-drug-loaded composite nano particle and preparation method and use thereof

A composite nanoparticle and nanoparticle technology, which is applied in the field of temperature/pH responsive double drug-loaded composite nanoparticle and its preparation and application, can solve problems such as unsatisfactory curative effect, decrease in white blood cell count, and hinder the proliferation of cancer cells. significant effect

Active Publication Date: 2019-10-29
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Generally, surgical resection combined with chemotherapy is used to prevent the spread of cancer cells. This treatment method has a considerable therapeutic effect, but the adverse effects after chemotherapy are also very large, among which the reduction in the number and quality of white blood cells is one of them.
[0003] Although there are many drugs and means for treating cancer at present, their curative effects are not satisfactory due to various reasons. Therefore, it is urgent to develop a targeted cancer therapy that is suitable for large-scale promotion, benefits the common people, has high efficiency, and has few side effects. medicine

Method used

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  • Temperature/pH responsive double-drug-loaded composite nano particle and preparation method and use thereof
  • Temperature/pH responsive double-drug-loaded composite nano particle and preparation method and use thereof
  • Temperature/pH responsive double-drug-loaded composite nano particle and preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1 Preparation method of temperature- and pH-responsive dual-loaded mesoporous silica nanoparticles drug carrier LEBM (1:1)

[0105] 1. Preparation of mesoporous silica nanoparticles (MSNs)

[0106] Add 0.2 g of cetyltrimethylammonium bromide (CTAB) and 10 mL of distilled water to a round-bottomed flask, stir magnetically for 5 min at 40 °C, then add 90 mL of water and 3 mL of ammonia in sequence, and continue magnetically stirring at 40 °C for 2 h , 500 rev / min. Then, 0.5 mL of methyl orthosilicate (TEOS) and 5 mL of ethyl acetate (EA) were added quickly, and after reacting for 6 h, it was cooled to room temperature, and washed three times with water and ethanol successively to obtain a mixture. Weigh 0.5g of ammonium nitrate and dissolve it in 50mL of ethanol, add the above mixture, and reflux at 80°C for 45min. Washed three times with ethanol and water successively to obtain 55.88 mg of mesoporous silica nanoparticles (MSNs), which were dried and stored in v...

Embodiment 2

[0118] The preparation of the double drug-loaded composite nanoparticle of embodiment 2 different proportions

[0119] According to the drug loading of EVO and BBR, by regulating the loading of EVO and BBR, the ratio of EVO and BBR is 6:1 dual response type composite nanoparticles synthesized by the drug loading method described in Example 1. That is, the double-loaded nanoparticles of EVO:BBR (6:1) are LEBM (6:1).

Embodiment 3

[0120] Embodiment 3 Preparation of double drug-loaded composite nanoparticles in different proportions

[0121] According to the drug loading of EVO and BBR, by adjusting the loading of EVO and BBR, the ratio of EVO and BBR is 1:6 dual response type composite nanoparticles synthesized by the drug loading method described in Example 1. That is, the double drug-loaded nanoparticles of EVO:BBR(1:6) are LEBM(1:6).

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Abstract

The invention discloses a temperature / pH responsive double-drug-loaded composite nano particle and a preparation method and use thereof. The temperature / pH responsive double-drug-loaded composite nanoparticle is obtained by sequentially preparing BBR@MSNp (NIPAM-co-MA), an LB film and LEBM. The preparation method comprises the steps that berberine (BBR) is loaded onto a specific-temperature and pH double-response functional nano particle, and then the nano particle is creatively coated with evodiamine (EVO), and the temperature / pH responsive double-drug-loaded composite nano particle is prepared. In vitro and in vivo experiments show that a targeted drug delivery system can accurately locate to a tumor site and achieve effective drug release. The EVO and BBR are subjected to combined administration through a temperature / pH responsive double-drug-loaded nano drug delivery system for the treatment of cancer, especially, a synergistic effect on the treatment of liver cancer, cervical cancer and breast cancer is achieved, and the curative effect is remarkable.

Description

technical field [0001] The invention relates to a temperature / pH responsive double drug-loaded composite nanoparticle, a preparation method and application thereof. Background technique [0002] Cancer seriously threatens human health and life. Among them, the incidence rate of liver cancer is about 25.7 / 100,000, becoming the third most malignant tumor in terms of mortality after gastric cancer and lung cancer. Currently, the treatment of liver cancer mainly adopts local therapy, including TACE, PEI, radiofrequency ablation, radiotherapy and surgery. Various treatment methods have their advantages and disadvantages and indications, and all have different degrees of side effects. For example, patients after TACE are often complicated with fever, pain, vomiting and liver function damage, and the postoperative quality of life is low, the body function is significantly reduced, and the immunity is weakened. TACE, PEI, radiofrequency ablation, local radiation therapy, and surg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/04A61K47/24A61K47/32A61K31/519A61K31/4375A61P35/00
CPCA61K9/5115A61K9/5138A61K9/5146A61K31/4375A61K31/519A61K2300/00
Inventor 吴敏冯易殷欢莉李泞汐陈天宇杨倩张全
Owner CHENGDU MEDICAL COLLEGE
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