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Functional guidance muscle tissue repair membrane and preparation method and application thereof

A muscle tissue and functionalization technology is applied in the field of functionalized guided muscle tissue repair membrane and its preparation, which can solve problems such as poor mechanical strength of hydrogel materials.

Active Publication Date: 2019-11-05
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, hydrogel materials have poor mechanical strength, so they can only be used for the repair of soft tissue, nerve tissue, and skin.

Method used

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  • Functional guidance muscle tissue repair membrane and preparation method and application thereof
  • Functional guidance muscle tissue repair membrane and preparation method and application thereof
  • Functional guidance muscle tissue repair membrane and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] (1) dissolving polycaprolactone and collagen in hexafluoroisopropanol, stirring magnetically at room temperature for 24 hours, to obtain a solution with a mass fraction of 15%;

[0064] (2) Electrospinning the mixed solution of the above-mentioned polycaprolactone and collagen, with a stainless steel drum as the receiving device, the drum rotation rate is 1000rpm, the spinning solution flow rate is 10mL / h, the voltage is 25kV, and the receiving distance is 20cm, The needle type of the syringe was 25G, the spinning temperature was room temperature, and the spinning was performed for 10 hours to obtain an electrospun fiber membrane PC with a thickness of 50 μm.

[0065] Such as figure 1 As shown, the diameter of the PC spun fiber membrane prepared in Example 1 is about 2 μm.

Embodiment 2

[0067] (1) Mix the protein gel with coagulation function modified by polyethylene glycol with the protein that promotes cell growth, dissolve in the mixed solution of ethanol and water, the volume ratio of ethanol and water is 5:5, the concentration of protein gel 10w / v%, the concentration of the protein that promotes cell growth is 40ng / ml, at the same time, add 0.1w / v% photoinitiator Irgacure 2959, stir under dark conditions, and make a gel;

[0068] (2) Glue was added to the dried electrospun fiber membrane prepared in Example 1, and irradiated with a long-wavelength ultraviolet lamp for 15 minutes to prepare the electrospun fiber membrane PFPC-C.

[0069] The morphology observation figure of the PC made in embodiment 1 and the PFPC-C made in embodiment 2 are as follows figure 2 shown.

[0070] Both the PC spun fiber membrane and the PFPC-C spun fiber membrane are red, and the red color is the color of DMEM medium; in addition, the surface of the PC spun fiber membrane is...

experiment example 1

[0072] The membrane prepared in Example 1-2 was punched into discs of 48-well plate size, spread in a 48-well plate, soaked in 75% ethanol for 30 minutes, irradiated with ultraviolet light for 10 minutes, sealed with a parafilm, and stored at 4°C; In the logarithmic phase, digest L6 cells, inoculate in 48-well plates with two kinds of membranes and no membranes, culture for 1d, 3d, and 7d, and inoculate each well with 3×10 5 Add 600 μl of culture medium to each well (3d, 5d and 7d are performed every other day and a half, that is, discard 300 μl of medium and supplement with 300 μl of fresh medium). CTGF connective tissue growth factor was directly added to the parallel blank group without membrane; the membrane was taken out with tweezers and placed in a new 48-well plate, the culture medium in the two plates was sucked out, and 220 μL of 10% CCK-8 (CCK-8 is diluted with serum-free DMEM medium), incubate for 1-4 hours, until the color of the solution turns brownish yellow, co...

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Abstract

The invention discloses a functional guidance muscle tissue repair membrane and a preparation method and application thereof. The functional guidance muscle tissue repair membrane comprises an irregularly arrayed micron-scale fiber membrane which is prepared through electrostatic spinning and by taking polycaprolactone and collagen as base materials, wherein the fiber diameter of the irregularly arrayed micron-scale fiber membrane is 1-10 [mu]m, and the membrane thickness of the irregularly arrayed micron-scale fiber membrane is 50-500 [mu]m; and the functional guidance muscle tissue repair membrane can further comprise a protein hydrogel loaded on the repair membrane and having a coagulation function, and a protein bound on the hydrogel and promoting growth of cells. The functional guidance muscle tissue repair membrane has excellent biocompatibility and mechanical properties, and can effectively promote regeneration and repair of muscle tissue, and the preparation method of the functional guidance muscle tissue repair membrane is scientific and simple, the technological process is controllable, and the functional guidance muscle tissue repair membrane is widely applied to preparation of drugs for promoting regeneration and repair of the muscle tissue.

Description

technical field [0001] The invention belongs to the field of biomaterials, and in particular relates to a functionalized guided muscle tissue repair membrane and its preparation method and application. Background technique [0002] Injury to skeletal muscle is a major topic currently facing the scientific community. Although protective facilities can be adopted to reduce the risk of injury, however, the safety of these facilities is often questioned. Once the skeletal muscles in the joints and knees are injured, it needs to be repaired by transplanting the injured's own skeletal muscles, and the postoperative recovery time is very long. injury. Medical staff also tried to use artificial materials to replace tissues, but these artificial materials could not normally integrate with their own muscle tissue due to rejection by the body after implantation. How to overcome the immune rejection of body transplantation, thoroughly solve the biocompatibility problem of artificial s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/18A61L27/24A61L27/54A61L27/22
CPCA61L27/50A61L27/18A61L27/24A61L27/54A61L27/227A61L2300/252A61L2300/414C08L67/04
Inventor 陈春英刘颖郭梦雨刘柱
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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