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Microstrip ring dendritic starch derivative and processing method thereof

A technology of starch derivatives and processing methods, which is applied in the field of food processing, can solve the problems of low starch modification degree, high modification cost, and waste pollution, and achieve good water solubility, low cost, and controllable reaction conditions.

Active Publication Date: 2019-11-05
JIANGNAN UNIV
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the limitation of the semi-crystalline structure of native starch, which limits its application range and application effect, scientific and technological workers around the world use various methods to modify the structure and function of starch. For example, the physical modification process is simple and easy to operate, but the physical modification The degree of modification of starch is not high, and often needs to be combined with other modification methods; chemically modified starch is currently the largest amount used in the starch industry, but its modification cost is high and the waste generated pollutes the environment; enzyme modified Mild reaction conditions, high reaction efficiency, strong substrate specificity, and environmental protection

Method used

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  • Microstrip ring dendritic starch derivative and processing method thereof
  • Microstrip ring dendritic starch derivative and processing method thereof
  • Microstrip ring dendritic starch derivative and processing method thereof

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preparation example Construction

[0047] Preparation of transglycosidase preparations:

[0048] A. Plant-derived transglycosidase preparation: obtained by extracting the endosperm of grain grains in the growth phase, weighing 100 g of grain grains in the filling phase, adding 300 mL of phosphate buffer (pH 7.2, 50 mM), and obtaining through homogenization, filtration, and centrifugation The crude enzyme solution is separated and purified by ion exchange column and gel chromatography, and the active ingredients are collected and freeze-dried to obtain the enzyme preparation. The plant grain endosperm includes: rice grain endosperm, wheat grain endosperm, corn grain endosperm, sorghum grain endosperm and the like.

[0049] B. Microbial-derived transglycosidase preparation: adopt the steps of archaea or bacteria obtained from natural screening through activation culture, fermentation and enzyme production, among which the bacteria are activated: under sterile conditions, take 200 μL of bacterial solution preserve...

Embodiment 1

[0051] Weigh 25g of degreased waxy cornstarch and suspend it in 10mL of absolute ethanol, continue to add 100mL of boric acid catalyst solution (pH2.5) and react at 40°C for 120min. After the reaction, neutralize the pH value, grade precipitation, wash and dry;

[0052] The resulting starch degradation product was dissolved in 100 mL of phosphate buffer (pH 7.0) to form a solution with a mass concentration of 2%, placed in a water bath at 70°C and heated for 30 min, and then 600 U of rice-derived transglycosidase preparation (sugar chain branched Depolymerization activity / depolymerization activity=52) and keep warm for 16 hours to heat inactivate the enzyme, centrifuge, and vacuum-dry the obtained supernatant to obtain the target product of dendritic starch with microstrip rings.

[0053] The obtained microstrip ring dendritic starch derivative has an average molecular weight of 4200 Da, wherein the average DP of the ring structure is 21, the proportion of α-1,6 glycosidic bond...

Embodiment 2

[0055] Weigh 20g of degreased waxy rice starch and suspend it in 8mL of absolute ethanol, continue to add 80mL of hydrochloride solution (pH4.0) and react at 50°C for 90min. After the reaction, neutralize the pH value, fractionally precipitate, wash and dry ;

[0056] Dissolve the obtained starch degradation product in 75mL phosphate buffer (pH 7.0) to form a solution with a mass concentration of 15%, place it in a water bath at 70°C and heat it for 40min, then add 800U of Bacillus stearothermophilus-derived transglycosidase preparation (sugar chain branching activity / depolymerization activity=31) and keep warm for 12 hours; heat to inactivate the enzyme, centrifuge, and vacuum-dry the obtained supernatant to obtain the target product.

[0057] The obtained microstrip ring dendritic starch derivative has a molecular weight of 3500 Da, the average DP of the ring structure is 20, the proportion of α-1,6 glycosidic bonds is 5.2%, and the yield is 9.7%.

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Abstract

The invention discloses a microstrip ring dendritic starch derivative and a processing method thereof, and belongs to the technical field of food processing. With starch as a raw material, the microstrip ring dendritic starch derivative is prepared through carbohydrate chain degradation and grading and glucoamylase catalysis glycoside conversion technologies and can be used as a stable carrier material of a food active factor. The method has the advantages of green environmental protection, high processing yield, low cost and the like; and the prepared product has the advantages of high branching degree, special large ring structure and good water solubility, can be applied to steady-state delivery and active protection of natural functional substances, and relates to the fields of nutritional food, medicine, daily chemicals and the like.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a microstrip ring dendritic starch derivative and a processing method thereof. Background technique [0002] As the second largest renewable resource in nature after cellulose, starch has the characteristics of cheap, easy to obtain, degradable and easy to derivatize. As a large agricultural country, our country is rich in starch resources. The total annual output has exceeded 27.2 million tons, but more than 90% of it is used to produce primary products such as starch sugar, sugar alcohols, and fermented products. There is still a big gap with developed countries, mainly reflected in The stability of product quality is not as good as that of foreign products, the utilization rate of raw materials is low, and the production technology is not perfect, especially the quality of high value-added products is lacking. [0003] At present, all countries in the worl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/18C12P19/04C12N9/10
CPCC12N9/1048C12P19/04C12P19/18
Inventor 缪铭江波陈琛刘瑶张涛
Owner JIANGNAN UNIV
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