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A new type of biofloc and its application and utilization method for roughing Litopenaeus vannamei

A new type of biological flocculation technology, applied in the agricultural field, can solve the problems of endangering the normal life activities of shrimp, large amount of shrimp compound feed, degradation of germplasm resources, etc., so as to reduce the risk of eutrophication and the breeding of pathogenic microorganisms, reduce Effects of aeration intensity and water exchange, improving immunity and disease resistance

Active Publication Date: 2021-12-31
TIANJIN AGRICULTURE COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the improvement of farming scale and intensification, problems such as deterioration of water body environment, frequent occurrence of diseases, and degradation of germplasm resources have become prominent, seriously restricting the sustainable development of Litopenaeus vannamei farming industry
At present, although biofloc technology has been widely used in Litopenaeus vannamei culture, there are still a series of problems: 1. The core component of traditional biofloc is heterotrophic bacteria, and the C / N suitable for its growth and reproduction is at 10 Above, but the C / N of the general culture pond is about 4-5, which is far from meeting the growth needs of heterotrophic bacteria. Therefore, in practical applications, a large amount of carbon source substances must be added to the pond to promote the growth and reproduction of heterotrophic bacteria. If the amount is not properly controlled It will cause eutrophication of the water body and the breeding of pathogenic microorganisms, which will seriously deteriorate the water quality and endanger the normal life activities of prawns; 2. The number of photosynthetic and oxygen-evolving organisms such as microalgae in traditional bioflocs is very small, and the mass reproduction of heterotrophic bacteria will aggravate The dissolved oxygen load in the water body leads to the shortage of dissolved oxygen in the water body, and then affects the life activities of floc organisms through negative feedback, greatly weakening the environmental regulation and bait supplementation of bioflocs; 3. Bioflocs containing a large number of dead bacteria in the middle and late stages of breeding If it is not cleaned up in time, it will cause secondary pollution of the water body, and a large number of water changes will cause stress and stress on the shrimp, which will seriously affect the growth of the shrimp and even cause death. At the same time, the tail water produced by the farming will greatly increase the environmental load.
[0007] The bioflocs in this publication are mainly heterotrophic bacteria, and a large amount of carbon source substances are required to be added during the operation of the system. If the dosage is not well grasped, it will easily cause eutrophication of the water body, resulting in a large number of harmful microorganisms, and at the same time increase the dissolved oxygen load of the water body. To maintain the normal operation of the system requires continuous and high-intensity aeration, which pushes up the cost of farming
Litopenaeus vannamei juveniles prefer plant-based bait during the growth stage. In this technology, the biofloc mainly composed of bacteria can not achieve good bait concentration and food supplementation, and the amount of compound feed used in the breeding process of prawns is too large

Method used

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  • A new type of biofloc and its application and utilization method for roughing Litopenaeus vannamei
  • A new type of biofloc and its application and utilization method for roughing Litopenaeus vannamei
  • A new type of biofloc and its application and utilization method for roughing Litopenaeus vannamei

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Experimental program
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Effect test

preparation example Construction

[0054] ⑵Preparation of microalgae aggregates and control of particle size

[0055] ① After mixing the Chlorella vulgaris / Pyramidella subcordiformis, Haematococcus pluvialis and Thalassiosira wischii of diatoms in step 1, centrifuge at 2000r / min for 15min, discard the supernatant, and collect Concentrate the algae liquid, mix the concentrated algae liquid with the transglutaminase liquid with a mass concentration of 2% in a volume ratio of 1:3, and vortex for 1 hour to obtain a mixture, which is ready for use;

[0056] ② Prepare alginate with a mass concentration of 0.1%-1% with water and set aside;

[0057] ③ water preparation mass concentration is 5% calcium chloride solution, for subsequent use;

[0058] ④ Mix the mixture in step ⑵① with the alginate in step ⑵② in a volume ratio of 1:1 to obtain the mixture;

[0059] ⑤ Add the mixture in step ⑵ ④ dropwise to the calcium chloride solution in step ⑵ ③ with a dropper to obtain microspherical microalgae aggregates.

[0060] P...

Embodiment 1

[0094] The roughness test was carried out in a blue plastic breeding box of 2m×1.5m×1.2m (length×width×height). Before releasing the seedlings, use potassium permanganate to thoroughly disinfect the culture box, and then inject sand-filtered and sterilized seawater to keep the water depth at 0.7m. Nano-aeration tubes are arranged in the same direction around the box, 0.1m and 0.4m away from the bottom of the box respectively.

[0095] Add 45g of molasses to the box before putting the seedlings to adjust the C / N of the water body to about 12-14, and at the same time inoculate the mixed microalgae aggregates, W-TJ01 and Z-QS01 bacterial liquid, and the inoculation density of the mixed microalgae aggregates is 25 grains / mL (containing Chlorella vulgaris with a cell density of 5×10 4 cells / mL, both Haematococcus pluvialis and Thalassiosira wilsonii are 1×10 5 cells / mL), the inoculation density of W-TJ01 and Z-QS01 was 1×10 4 CFU / mL and 3×10 4 CFU / mL. Sufficient aeration to f...

Embodiment 2

[0099] The standard roughness test was carried out in a steel frame greenhouse. The pond area in the shed was 1.5 mu. It was equipped with bottom-filled aeration equipment and was inflated with a blower. The PVC pipe was an inflatable pipe (pipe diameter 15mm), and the distance between the pipes was 4m. In addition, the distance between the PVC pipes was 0.6 m perforation, the hole diameter is 0.6mm, and the configuration power of the oxygenation device is 0.2kW / mu. Before putting the seedlings, the whole pond is cleaned and disinfected, and then injected into the seawater that has been naturally precipitated for 24 hours and filtered through an 80-mesh sieve. Fully aerate to remove residual chlorine. Sprinkle 0﹟ standard coarse special material (9kg / mu) and molasses (5kg / mu) in the whole pond before putting seedlings, adjust the water body C / N to about 13-15, and at the same time inoculate mixed microalgae aggregates, W-TJ01 and Z -QS01 bacteria liquid, in which the inoculat...

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Abstract

The present invention relates to a novel biofloc, which includes microalgae aggregates and commensal heterotrophic bacteria, and the commensal heterotrophic bacteria are bacteria of the genus Pseudomonas (Pesudomonas sp.) and bacteria of the genus Bacillus ( Bacillus sp.). The biofloc has significantly improved the water quality of the Litopenaeus vannamei standard coarse pond, improved the survival rate, growth index and immunity of the prawn, and reduced the application of compound feed and external carbon source, and reduced the intensity of aeration and replacement. water volume.

Description

technical field [0001] The invention belongs to the technical field of agriculture, in particular to a novel biofloc and a method for applying and utilizing the same to standardize Litopenaeus vannamei. Background technique [0002] In 1999, Israeli scientist Avnimelech first proposed the application of Biofloc Technology (BFT) in the field of aquaculture. After nearly two decades of development, BFT has become a commonly used environmental regulation in aquaculture (especially crustacean farming). technology. The main point of the application of BFT is to adjust the C / N of the water body by artificially adding carbon sources, thereby promoting the formation of flocs in probiotics (such as bacillus, lactic acid bacteria, yeast, nitrifying bacteria, etc.), microalgae and protozoa in the water, and then Use floc biology to remove nitrogen, phosphorus, nutrients and excess organic matter in the water body, and convert them into bacteria / algae protein, and then become high-qual...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/20C02F3/34C02F3/32A01K61/59A23K50/80A23K10/18C12R1/89C12R1/38C12R1/07C02F103/20
CPCC12N1/12C12N1/20A01K61/59A23K50/80A23K10/18C02F3/34C02F3/325C02F2103/20Y02A40/81
Inventor 窦勇周文礼王墨染王晴晴邵蓬高金伟于士国贾旭颖
Owner TIANJIN AGRICULTURE COLLEGE
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