A new type of biofloc and its application and utilization method for roughing Litopenaeus vannamei
A new type of biological flocculation technology, applied in the agricultural field, can solve the problems of endangering the normal life activities of shrimp, large amount of shrimp compound feed, degradation of germplasm resources, etc., so as to reduce the risk of eutrophication and the breeding of pathogenic microorganisms, reduce Effects of aeration intensity and water exchange, improving immunity and disease resistance
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[0054] ⑵Preparation of microalgae aggregates and control of particle size
[0055] ① After mixing the Chlorella vulgaris / Pyramidella subcordiformis, Haematococcus pluvialis and Thalassiosira wischii of diatoms in step 1, centrifuge at 2000r / min for 15min, discard the supernatant, and collect Concentrate the algae liquid, mix the concentrated algae liquid with the transglutaminase liquid with a mass concentration of 2% in a volume ratio of 1:3, and vortex for 1 hour to obtain a mixture, which is ready for use;
[0056] ② Prepare alginate with a mass concentration of 0.1%-1% with water and set aside;
[0057] ③ water preparation mass concentration is 5% calcium chloride solution, for subsequent use;
[0058] ④ Mix the mixture in step ⑵① with the alginate in step ⑵② in a volume ratio of 1:1 to obtain the mixture;
[0059] ⑤ Add the mixture in step ⑵ ④ dropwise to the calcium chloride solution in step ⑵ ③ with a dropper to obtain microspherical microalgae aggregates.
[0060] P...
Embodiment 1
[0094] The roughness test was carried out in a blue plastic breeding box of 2m×1.5m×1.2m (length×width×height). Before releasing the seedlings, use potassium permanganate to thoroughly disinfect the culture box, and then inject sand-filtered and sterilized seawater to keep the water depth at 0.7m. Nano-aeration tubes are arranged in the same direction around the box, 0.1m and 0.4m away from the bottom of the box respectively.
[0095] Add 45g of molasses to the box before putting the seedlings to adjust the C / N of the water body to about 12-14, and at the same time inoculate the mixed microalgae aggregates, W-TJ01 and Z-QS01 bacterial liquid, and the inoculation density of the mixed microalgae aggregates is 25 grains / mL (containing Chlorella vulgaris with a cell density of 5×10 4 cells / mL, both Haematococcus pluvialis and Thalassiosira wilsonii are 1×10 5 cells / mL), the inoculation density of W-TJ01 and Z-QS01 was 1×10 4 CFU / mL and 3×10 4 CFU / mL. Sufficient aeration to f...
Embodiment 2
[0099] The standard roughness test was carried out in a steel frame greenhouse. The pond area in the shed was 1.5 mu. It was equipped with bottom-filled aeration equipment and was inflated with a blower. The PVC pipe was an inflatable pipe (pipe diameter 15mm), and the distance between the pipes was 4m. In addition, the distance between the PVC pipes was 0.6 m perforation, the hole diameter is 0.6mm, and the configuration power of the oxygenation device is 0.2kW / mu. Before putting the seedlings, the whole pond is cleaned and disinfected, and then injected into the seawater that has been naturally precipitated for 24 hours and filtered through an 80-mesh sieve. Fully aerate to remove residual chlorine. Sprinkle 0﹟ standard coarse special material (9kg / mu) and molasses (5kg / mu) in the whole pond before putting seedlings, adjust the water body C / N to about 13-15, and at the same time inoculate mixed microalgae aggregates, W-TJ01 and Z -QS01 bacteria liquid, in which the inoculat...
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