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Self-aggregation granular sludge construction method for cascade energy recovery of wastewater

A technology for granular sludge and energy recovery, applied in microorganism-based methods, biochemical equipment and methods, bioreactors/fermenters for specific purposes, etc. , long start-up time and other problems, to achieve the effect of rapid continuous and stable hydrogen production, shorten start-up time, and reduce hydrogen production costs

Pending Publication Date: 2019-12-31
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention provides a waste water cascade to solve the problems of complex composition, long start-up time, low hydrogen production efficiency, strict anaerobic conditions, cumbersome operation, high cost, and unfavorable expansion of biological hydrogen production in the existing mixed culture system. Self-aggregating granular sludge construction method for energy recovery

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  • Self-aggregation granular sludge construction method for cascade energy recovery of wastewater
  • Self-aggregation granular sludge construction method for cascade energy recovery of wastewater

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specific Embodiment approach 1

[0023] Specific embodiment 1: In this embodiment, a self-aggregating granular sludge construction method for waste water cascade energy recovery is provided. The specific operation steps of the method are as follows:

[0024] (1) Aerobic cultivation of Pseudomonas aeruginosa PqsL in a constant temperature shaker: use liquid medium A sterilized by high temperature and high pressure steam to cultivate Pseudomonas aeruginosa PqsL, and place the inoculated Erlenmeyer flask in a constant temperature shaker Vibrating culture in the bed, wherein, the temperature of the constant temperature shaker is 35°C, and the rotation speed is 100rpm. After 12 hours, a light yellow turbid bacterial liquid with an absorbance of 0.9-1.0 at a wavelength of 600nm is formed. Take 100ml-300ml of light yellow turbid bacterial liquid, 12000r Centrifuge for 1 minute at / h, discard the supernatant, and obtain the inoculum of Pseudomonas aeruginosa PqsL; wherein each 1L of liquid medium A consists of 2.0 g o...

specific Embodiment approach 2

[0030] Specific embodiment 2: This embodiment provides a cultivation method of Harbin ethanologen YUAN-3 in a continuous flow biological hydrogen production reactor. The main difference between the operation steps of this method and specific embodiment 1 is that only inoculation The other culture conditions and steps of the inoculum of Harbin ethanologen YUAN-3 are the same, and the method starts to produce hydrogen after 40 hours of culture, and the maximum hydrogen production rate is 1.62mol-hydrogen / mol-sucrose.

[0031] The hydrogen production effect of specific embodiment 1 and specific embodiment 2 is compared in the following table,

[0032]

[0033] It can be seen from the above table that inoculating the self-aggregated granular sludge of Harbin ethanologen YUAN-3 and Pseudomonas aeruginosa PqsL in the continuous flow biohydrogen production reactor can significantly shorten the start-up time of the continuous flow biohydrogen production reactor Simultaneously, the ...

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Abstract

The invention discloses a self-aggregation granular sludge construction method for cascade energy recovery of wastewater and belongs to the technical field of hydrogen production by fermentation. Theinvention aims to solve the problems that existing mixed culture systems for wastewater treatment are complex in composition, long in start time, low in energy efficiency, complex to operate, high incost and not beneficial to enlargement and the like. A continuous-flow biological hydrogen production reactor is inoculated with self-aggregation granular sludge formed by co-culture of ethanoligenensharbinense YUAN-3 and pseudomonas aeruginosa PqsL, the reactor is quickly started by microbial interaction, and energy such as hydrogen and ethanol is efficiently recovered during wastewater treatment. The pseudomonas aeruginosa PqsL can quickly consume oxygen in the continuous-flow biological hydrogen production reactor and provide a proper anaerobic environment for the ethanoligenens harbinenseYUAN-3, so that the ethanoligenens harbinense YUAN-3 quickly and continuously produce hydrogen in the continuous-flow biological hydrogen production reactor without anaerobic treatment, and the starttime of the reactor is shortened while the reaction condition is simplified.

Description

technical field [0001] The invention relates to a method for constructing self-aggregating granular sludge for waste water cascade energy recovery, and belongs to the technical field of fermentation hydrogen production. Background technique [0002] Fermentative biological hydrogen production can use organic wastewater to produce clean and efficient hydrogen energy at normal temperature and pressure, and has many advantages such as high hydrogen production rate, eco-friendliness, and low cost. As an important energy carrier, hydrogen has high combustion density, clean and sustainable, and is a promising alternative to fossil fuels. [0003] Most anaerobic fermentation biohydrogen production systems are mixed culture systems that use pretreated anaerobic activated sludge from different wastewater sources as the inoculum. Its advantages are convenient inoculation, simple operation, and no need for sterilization during the entire operation process. However, the mixed culture s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/06C12P3/00C12M1/00C12M1/107C12R1/01C12R1/385
CPCC12P39/00C12P7/06C12P3/00C12M21/04C12M21/12C12M23/36C12M29/18C12M47/02Y02P20/59Y02E50/10
Inventor 邢德峰李真
Owner HARBIN INST OF TECH
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