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Application of sanicula astrantiifolia wolff extract in preparation of blood fat reducing drugs

A small black medicine and extract technology, applied in the field of blood lipid-lowering drugs, can solve the problems of poor long-term curative effect and difficulty in reversing diseases, and achieve high safety and good lipid-lowering effects

Active Publication Date: 2020-01-14
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current lifestyle intervention and allopathic treatment for disease symptoms have poor long-term efficacy and are difficult to reverse the development of the disease, highlighting the importance of developing new intervention strategies and prevention and treatment drugs

Method used

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  • Application of sanicula astrantiifolia wolff extract in preparation of blood fat reducing drugs
  • Application of sanicula astrantiifolia wolff extract in preparation of blood fat reducing drugs
  • Application of sanicula astrantiifolia wolff extract in preparation of blood fat reducing drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Preparation of Alcoholic Extract and Middle and Low Polarity Parts of Xiaoheiyao

[0030] Prepared according to the following method:

[0031] (1) Preparation of the alcohol extract of Xiaoheiyao: 100g of dried rhizome of Xiaoheiyao is crushed by a medicinal material grinder (Huangcheng HC-2500Y304, Wuyi Haina Electric Co., Ltd.), passed through a 100-mesh sieve, and added at a mass-volume ratio of 1:20 95% ethanol (analytically pure, Changzhou Hengguang Chemical Reagent Co., Ltd.), put it in an ultrasonic cleaning machine (Dongsen DS-100S, Shenzhen Pinhuang Technology Co., Ltd.) for ultrasonic extraction for 1 hour, centrifuge to get the supernatant and carry out a rotary evaporator (Shanghai Yarong RE-52A, Shanghai Yarong Biochemical Instrument Factory) Concentrate to dryness under reduced pressure to obtain 9.5 g of the ethanol extract of Xiaoheiyao.

[0032] (2) Extraction of middle and low polar parts: take 9.0g of Xiaoheiyao ethanol extract and dispers...

Embodiment 2

[0033] Example 2: Lipid-lowering effect of the alcoholic extract of Xiaoheiyao and various polar parts (in vitro experiment)

[0034] Sodium oleate and sodium palmitate (Sigma-Aldrich) with a molar ratio of 1:2 were coupled with fatty acid-free bovine serum albumin (Shanghai Yisheng Biotechnology Co., Ltd.) and mixed to obtain mixed free fatty acids. Add 1.2mM mixed free fatty acids to the human liver cancer HepG2 cells (Cell Bank of Type Culture Preservation Committee, Chinese Academy of Sciences) in the 96-well plate, in 5% CO at 37°C. 2The cells were cultured in a cell incubator (Forma, Thermo Scientific) for 24 hours to establish a lipid accumulation model induced by free fatty acids. By investigating the effects of the Xiaoheiyao alcohol extract (XHY-Q), petroleum ether fraction (PE-Q), ethyl acetate fraction (AE-Q), and water fraction (W-Q) prepared in Example 1 on the proliferation of HepG2 cells After that, it is determined that the appropriate administration concentr...

Embodiment 3

[0042] Example 3: Lipid-lowering effect of the alcoholic extract of Xiaoheiyao (in vivo experiment)

[0043] 40 4-week-old ICR male mice with an initial body weight of 30 g (Shanghai Slack Experimental Animal Co., Ltd.) were adaptively fed for 1 week, and were randomly divided into 4 groups, 10 in each group, respectively normal group CON and high-fat group HFD, high-fat black medicine alcohol extract HFD+XHY-Q. The CON group was given normal diet, and 0.2mL normal saline was fed daily; HFD was given high-fat diet (Jiangsu Synergy Pharmaceutical Bioengineering Co., Ltd.), and 0.2mL normal saline was fed daily; HFD+XHY-Q Give a high-fat diet (Jiangsu Synergy Pharmaceutical Bioengineering Co., Ltd.), and gavage the alcoholic extract of Xiaoheiyao at a daily rate of 20 mg / kg body weight. From the 5th week, the average body weight of the mice in the HFD group exceeded 130% of the average body weight in the CON group, indicating that the high-fat diet induced the successful establ...

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Abstract

The invention discloses an application of asanicula astrantiifolia wolff extract in preparation of a blood fat reducing drug, which belong to the technical field of blood fat reducing medicines. The sanicula astrantiifolia wolff extract is one or more of a sanicula astrantiifolia wolff alcohol extract, a sanicula astrantiifolia wolff petroleum ether part and a sanicula astrantiifolia wolff ethyl acetate part; a preparation method of the sanicula astrantiifolia wolff alcohol extract comprises the following steps: crushing dry rhizomes of sanicula astrantiifolia wolff by adopting a crusher, sieving the material, adding a C1-C4 alcohol solution with the volume percentage of 20-100%, extracting the material for 0.5-2 hours, centrifuging, taking a supernate, carrying out rotary evaporation, andconcentrating the material to obtain the sanicula astrantiifolia wolff alcohol extract. Compared with the prior art, the sanicula astrantiifolia wolff alcohol extract provided by the invention has the properties of high safety and good lipid-lowering effect, and can be applied to medicines, foods or functional foods for preventing and adjunctively treating obesity, alcoholic fatty liver, non-alcoholic fatty liver, type 2 diabetes, metabolic syndrome, coronary heart disease and atherosclerotic diseases.

Description

technical field [0001] The invention relates to the application of the extract of Xiao Hei Yao in the preparation of blood lipid-lowering medicine, and belongs to the technical field of blood fat-lowering medicine. Background technique [0002] Xiaoheiyao is the rhizome of Inula nervosa wall (Inula nervosa wall), also known as Yunweiling. It is a commonly used anti-inflammatory Chinese herbal medicine in the folk. Approved by the Ministry of Health as a new food raw material, it is a dual-purpose resource for medicine and food. Studies have shown that Inula inula is rich in phenols and volatile oil components, of which thymol and thymol isobutyrate are the main components in the volatile oil, and Yunweiling oil soft capsules developed from its volatile oil are used to treat rheumatism Bibing (Li Ke, Shi Leizhe, Chen Dan, Hu Yibing, Lu Maofang, Li Ruocun, Li Shunxiang. Determination of thymol and thymol isobutyrate in different medicinal parts, places of origin and harvestin...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P3/06A61P3/04A61P1/16A61P3/10A61P3/00A61P9/10A23L33/105A23K10/30
CPCA23V2002/00A61K36/28A61K2236/333A61K2236/55A23K10/30A23L33/105A61P1/16A61P3/00A61P3/04A61P3/06A61P3/10A61P9/10A23V2200/332A23V2200/328A23V2200/326A23V2200/30A23V2250/21
Inventor 成向荣董文乐赵蔚孙进乐国伟
Owner JIANGNAN UNIV
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