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Microalgae biomembrane culture method capable of simultaneously improving biomass yield and oil yield

A technology of microalgae biology and culture method, applied in the direction of microorganism-based method, biochemical equipment and method, method of supporting/immobilizing microorganisms, etc., can solve the problems of microalgae cell growth limitation, unfavorable nutrient transmission, etc., and achieve effective It is beneficial to the growth of microalgae, optimizes the ratio of dosage, and improves the effect of yield and oil content

Active Publication Date: 2020-01-14
CHONGQING UNIV
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Problems solved by technology

However, there are still some problems to be solved in this method of semi-dry adherent autotrophic culture of microalgae. For example, the dense biofilm is not conducive to the transmission of nutrients; light attenuation limits the growth of microalgal cells in the lower layer of the biofilm, seriously inhibiting Accumulation of biomass and lipids in microalgal biofilms

Method used

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  • Microalgae biomembrane culture method capable of simultaneously improving biomass yield and oil yield

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Experimental program
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Effect test

Embodiment 2

[0035] Embodiment 2: It is different from Embodiment 1. Step 1 uses Chlorella vulgaris (Chlorellavulgaris, FACHB-31) as the culture object; in step 3, select the combination of standard BG11 medium and 10g / L plus glucose carbon source to regulate and optimize the medium, so that the inorganic carbon and organic The ratio of carbon is 29:1 and the ratio of total carbon to total nitrogen is 96:1. In Step 4, the medium was sterilized in a sterilizing pot at 121° C. for 20 minutes and cooled to inoculate the microalgae biofilm, and then the microalgae biofilm was cultured at a constant temperature of 25° C. for 5 days.

[0036] After measurement, it is known that the biomass of Chlorella vulgaris microalgae biofilm is 79.80g / m 2 , the fat content is 21.65%. The specific measurement method is as follows:

[0037] Biomass measurement method: Use tweezers to remove the two microalgae membranes in the microalgae photobioreactor, add 10mL of first-grade deionized water to fully rins...

Embodiment 3

[0039] Example 3: The difference from Example 2 is that the ratio of inorganic carbon to organic carbon in the culture medium is 20:1, and the ratio of total carbon to total nitrogen is 96:1.

[0040] After measurement, it is known that the biomass of Chlorella vulgaris biofilm is 124.94g / m 2 , The oil content was 19.94%. Compared with the cultivation effect of Example 2, the biomass accumulation of the microalgae biofilm increased significantly by 56.57%, but the oil content decreased by 7.90%. Concrete measuring method is with embodiment 2.

example 4

[0041] Example 4: The difference from Example 2 is that the ratio of inorganic carbon to organic carbon in the medium is 29:1, and the ratio of total carbon to total nitrogen is 72:1.

[0042] After measurement, it is known that the biomass of Chlorella vulgaris biofilm is 75.22g / m 2 , the oil content was 33.43%. Compared with Example 2, the oil content of the microalgae biofilm increased significantly by 54.41%, but the biomass output of the biofilm was low, which decreased by 5.33%. Concrete measuring method is with embodiment 2.

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Abstract

The invention discloses a microalgae biomembrane culture method capable of simultaneously improving biomass yield and oil yield. The method is characterized by comprising the following steps of: step1, pre-culturing microalgae: inoculating a culture medium bottle with a trace amount of algae liquid for pre-culturing until microalgae cells grow to a later stage of logarithmic growth; step 2, immobilizing the microalgae cells: immobilizing the pre-cultured microalgae cells to obtain a microalgae biomembrane; step 3, regulating and optimizing a culture medium: adding a certain amount of organiccarbon source into a standard culture medium so as to realize the combination of photosynthetic autotrophy and heterotrophism in a culture process of the microalgae, and regulating and optimizing theratio of inorganic carbon to organic carbon and the ratio of total carbon to total nitrogen in the culture medium so as to realize water circulation and the increase of the biomass yield and oil yieldof the microalgae; step 4, performing inoculation; step 5, performing culturing by combining photosynthetic autotrophy and heterotrophism; and step 6, collecting cells. The method can be widely applied to the fields of energy, biology, especially microalgae industrial culture.

Description

technical field [0001] The invention relates to the field of microalgae biofilm cultivation, in particular to a microalgae biofilm cultivation method capable of simultaneously increasing biomass and oil production. Background technique [0002] "Industrial blood" - Petroleum fuel has brought social progress and economic prosperity to mankind, but it has also caused the double crisis of fossil energy depletion and global warming. Therefore, the development and utilization of an environmentally friendly alternative to fossil energy The new type of new color energy is very important. Among them, biomass energy, as a renewable energy that stores solar energy in the form of chemical energy in biomass, is inexhaustible and inexhaustible, and is very likely to become an important part of the future sustainable energy system. Algae are considered to be a potential raw material for biomass energy due to their advantages such as fast reproduction, short cycle, high photosynthetic eff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N11/00C12M1/00C12R1/89
CPCC12N1/12C12N11/00C12M21/02C12M25/02
Inventor 黄云陈科名廖强朱恂夏奡朱贤青
Owner CHONGQING UNIV
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