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Zinc oxide nanorod array cell culture substrate, and preparation method and application thereof

A zinc oxide nanorod, cell culture technology, applied in biochemical equipment and methods, cell culture supports/coatings, zinc oxide/zinc hydroxide, etc., can solve difficult cell expansion and dryness maintenance, hydrophobic micro The sphere is not conducive to cell adhesion, long cycle and other problems, and achieves the effect of maintaining stem/progenitor cell stemness, short cycle and good cell adhesion.

Active Publication Date: 2020-01-14
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, Chinese patent documents CN109675100A and CN109647298A respectively disclose the effects of polylactic acid-zinc oxide micro-nano multi-level structure composite microspheres and polyethylene-zinc oxide micro-nano multi-level structure composite microspheres on the expansion of stem cells and the maintenance of stem cell stemness. application, but the preparation process requires the use of irritating organic substances such as dichloromethane and polyvinyl alcohol. The material preparation step includes pre-preparing seed crystals, which takes a long period of time. Hydrophobic microspheres are not conducive to cell adhesion
Among them, the length of zinc oxide nanorods is 10-50nm. Due to the strong uptake of surrounding nanoparticles by stem / progenitor cells, within this size range, zinc oxide nanorods are easily phagocytized and dissolved by cells, resulting in rapid failure, and it is difficult to achieve long-term Cell expansion and stemness maintenance

Method used

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  • Zinc oxide nanorod array cell culture substrate, and preparation method and application thereof
  • Zinc oxide nanorod array cell culture substrate, and preparation method and application thereof
  • Zinc oxide nanorod array cell culture substrate, and preparation method and application thereof

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Effect test

Embodiment 1

[0038] A method for preparing a zinc oxide nanorod array cell culture substrate, the steps are as follows:

[0039](1) Cut off a 2cm x 2cm piece of aluminum foil, wrap the indium tin oxide conductive glass with a size of 1.5cm x 1.5cm, and expose the conductive indium tin oxide surface for growing zinc oxide nanorod arrays;

[0040] (2) At 25°C, prepare an aqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine, the molar ratio of zinc nitrate hexahydrate to hexamethylenetetramine is 1:1, zinc nitrate hexahydrate and hexamethylenetetramine The concentration of tetramine in aqueous solution is 25mM;

[0041] (3) Place the indium tin oxide conductive glass wrapped with aluminum foil in step (1) in the aqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine configured in step (2), and react in a water bath at 90° C. for 4 hours to obtain ZnO nanorod array cell culture substrate.

[0042] The zinc oxide nanorod array cell culture substrate prepared above ...

Embodiment 2

[0048] A method for preparing a zinc oxide nanorod array cell culture substrate, the steps are as follows:

[0049] (1) Cut off a 2cm x 2cm piece of aluminum foil, wrap the indium tin oxide conductive glass with a size of 1.5cm x 1.5cm, and expose the conductive indium tin oxide surface for growing zinc oxide nanorod arrays;

[0050] (2) At 25°C, prepare an aqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine, the molar ratio of zinc nitrate hexahydrate to hexamethylenetetramine is 1:1, zinc nitrate hexahydrate and hexamethylenetetramine The concentration of tetramine in aqueous solution is 30mM;

[0051] (3) Place the indium tin oxide conductive glass wrapped with aluminum foil in step (1) in the aqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine configured in step (2), and react in a water bath at 90° C. for 4 hours to obtain ZnO nanorod array cell culture substrate.

[0052] The zinc oxide nanorod array cell culture substrate prepared above...

Embodiment 3

[0057] A method for preparing a zinc oxide nanorod array cell culture substrate, the steps are as follows:

[0058] (1) Cut off a 2cm x 2cm piece of aluminum foil, wrap the indium tin oxide conductive film with a size of 1.5cm x 1.5cm, and expose the conductive indium tin oxide surface for growing zinc oxide nanorod arrays;

[0059] (2) At 25°C, prepare an aqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine, the molar ratio of zinc nitrate hexahydrate to hexamethylenetetramine is 1:1, zinc nitrate hexahydrate and hexamethylenetetramine The concentration of tetramine in aqueous solution is 25mM;

[0060] (3) Place the indium tin oxide conductive film wrapped with aluminum foil in step (1) in the aqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine configured in step (2), react in a water bath at 85°C for 4h, and obtain ZnO nanorod array cell culture substrate. Since the conductive thin film is a flexible substrate, the reaction temperature is sl...

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Abstract

The invention relates to a zinc oxide nanorod array cell culture substrate, and a preparation method and application thereof. An electric conduction substrate coated with an aluminum foil is put in anaqueous solution of zinc nitrate hexahydrate-hexamethylenetetramine to grow through a water bath method to obtain the zinc oxide nanorod array cell culture substrate. The preparation method is simple, and cost is low. A zinc oxide seed crystal layer does not need to be prepared on the substrate in advance, the diameter of a zinc oxide nanorod prepared on multiple substrates through the water bathmethod is 50-500nm, and the length of the zinc oxide nanorod is 0.5-5 [mu]m. The zinc oxide nanorod of a large size can not be easily taken and dissolved by cells, and can continuously take effects for a long time, and in addition, better cell adhesion is provided. The zinc oxide nanorod array cell culture substrate prepared with the preparation method disclosed by the invention has a good effecton maintaining the dry effect of dry / progenitor cells under a condition of long-term culture in vitro for 1-30 days or passage.

Description

technical field [0001] The invention relates to a zinc oxide nanorod array cell culture substrate and its preparation method and application, in particular to a zinc oxide nanorod array cell culture substrate for maintaining cell stemness and its preparation method and application, belonging to biomaterial technology field. Background technique [0002] In modern medicine, stem / progenitor cell research is one of the most promising fields and has greatly promoted the progress of life science and biomedicine. The ever-increasing demand for tissue regeneration and other stem / progenitor cell therapies requires a large number of stem / progenitor cells with self-renewal capabilities. However, under the conditions of long-term in vitro culture and multiple passages, the stemness of stem / progenitor cells will gradually decrease, that is, the proliferation rate will gradually decrease, and the ability of multidirectional or unidirectional directional differentiation will gradually be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C12N5/077C12N5/073C01G9/02B82Y40/00
CPCC12N5/0667C12N5/0663C12N5/0603C12N5/0654C01G9/02B82Y40/00C12N2533/20C01P2004/03C01P2004/50
Inventor 王书华孔颖桑元华王建军刘宏
Owner SHANDONG UNIV
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