Micro-injection glass needle breaking device and method for insects

Abstract

The invention belongs to the technical field of micro-injection, and particularly relates to a micro-injection glass needle breaking device and method for insects. A micro-injection glass needle breaking method for the insects includes the following steps of 1) fixing, wherein the tail of a micro-injection glass needle is fixed to a clamping groove, a needle tip is fixed into a tapered hole channel, and a connecting tube is connected to the tail of the glass needle; 2) needle breaking, wherein a sliding cutter is manually moved horizontally to vertically cut off the micro-injection glass needle; 3) needle grinding, wherein a movable cutting block is replaced with a movable grinding block, fine sandpaper is used for further finely grinding the needle tip of the micro-injection glass needlealong the plane of the movable grinding block; and 4) inspection, wherein the stable air pressure value inside the glass needle is detected by a detection device after the glass needle is broken. By means of the micro-injection glass needle breaking device and method, under the assistance of a magnifying glass and a sliding microscopy scaleplate, the opening diameter of the needle tip and the glass wall thickness of the needle breaking portion of the micro-injection glass needle are measured and adjusted in advance, and the stability of the opening diameter of the needle tip and the mechanicalstrength of the needle tip are stabilized.

Description

technical field [0001] The invention belongs to the field of microinjection technology, and in particular relates to a needle breaking device and method for microinjection glass needles used for insects. Background technique [0002] Microinjection is an important method for the research of insect gene silencing, insect gene editing, physiology, biochemistry and drug resistance mechanism. By microinjecting insect eggs, larvae and adults, quantitative injection of DNA, RNA, protein and chemical drugs can be directly injected into insects. [0003] The microinjection system is an important device for entomology researchers to perform microinjection operations. Microinjection glass needles are a key part of the microinjection system. However, in actual work, the following outstanding problems still exist in the process of making and using insect microinjection glass needles: [0004] Insect eggs, larvae, and adults are rich in hemolymph, and maintain their body shape through ...

AI Technical Summary

Problems solved by technology

However, even if the operator has practiced for a long time, the accuracy and stability of manual broken needles are still difficult to guarantee

Although the existing needle breaker can break the needle mechanically, the technology is complicated, and the consumables such as platinum wire are expensive, and the funds of conventional laboratories are difficult to afford. More importantly, the existing needle breaker is mainly for embryos and cells. Microinjection test design, the microinjection needle tip prepared by breaking the glass needle through thermal expansion and cold contraction of platinum wire is too small in diameter, and the needle tip strength is too low to meet the needs of insect microinjection test

Patent application number 201820385249.2, titled "A Simple Needle Breaking Device for Chicken Embryo Microinjection", although a mechanical needle breaking method is provided, the glass needle is not fixed during the cutting process, and the hand-held microinjection The needle breaking process of glass needles is difficult to ensure the consistency of the broken needle position and the broken needle angle of microinjection glass needles

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