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Reinforced ligament tissue engineering scaffold and preparation method for same

A tissue engineering scaffold and enhanced technology, applied in the field of medical devices, can solve the problem of slow decline in the mechanical properties of the scaffold, achieve good biocompatibility, eliminate microporous structure deformation, and ensure stability

Active Publication Date: 2017-03-08
WUHAN TEXTILE UNIV
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] In view of the above-mentioned problems, the purpose of the present invention is to solve the defects of the existing ligament tissue engineering scaffolds, using parallel silk threads or absorbable sutures as the reinforcement skeleton, and the reinforcement skeleton is nested in the adjacent electrospun nanofiber mat Between the layers, a reinforced ligament tissue engineering scaffold and its preparation method are provided to meet the requirements of the ligament tissue engineering scaffold to maintain the slow decline in the mechanical properties of the scaffold during the growth of new tissue and the slow degradation of nanofibers, and to ensure the stability of the connected joint

Method used

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  • Reinforced ligament tissue engineering scaffold and preparation method for same

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preparation example Construction

[0026] A method for preparing a reinforced ligament tissue engineering scaffold, comprising preparing a silk fibroin solution or a silk fibroin powder dispersion and a polyvinyl alcohol solution using water as a solvent, and the preparation method is carried out according to the following steps:

[0027]a. Mix the prepared silk fibroin solution or silk fibroin powder dispersion with water as solvent and polyvinyl alcohol solution to obtain a mixed solution, wherein the mass ratio of silk fibroin to polyvinyl alcohol is 1 / 9~1 / 1, the total solute mass concentration of silk fibroin and polyvinyl alcohol is 5wt% to 20wt%, and the mixed solution is sucked into the spinning solution tube for electrospinning;

[0028] b. Preparation of reinforced ligament tissue engineering scaffold by electrospinning

[0029] Using a flat metal strip with a width of 2-15mm and a length of 30-300mm as a collecting plate, place the spinning liquid tube for electrospinning that has absorbed the compos...

Embodiment 1

[0038] a. the silk fibroin aqueous solution with a mass concentration of 5wt% is mixed with a polyvinyl alcohol aqueous solution with a mass concentration of 5wt% to obtain a mixed solution, wherein the mass ratio of silk fibroin to polyvinyl alcohol is 1 / 9, silk fibroin and polyvinyl alcohol The total solute mass concentration of polyvinyl alcohol is 5wt%, and the mixed solution is sucked into the spinning liquid pipe used for electrospinning;

[0039] b. Preparation of reinforced ligament tissue engineering scaffold by electrospinning

[0040] Select a flat metal strip with a width of 2mm and a length of 30mm as the collecting plate, place the spinning solution tube for electrospinning that has absorbed the composite solution in the electrospinning push pump, and push the electrospinning solution at a speed of 0.2ml / hour. The high-voltage power supply outputs a voltage of 5kV. The distance between the nozzle of the spinning liquid pipe and the silk thread collection plate is...

Embodiment 2

[0043] a. the silk fibroin aqueous solution with a mass concentration of 8wt% is mixed with a polyvinyl alcohol aqueous solution with a mass concentration of 8wt% to obtain a mixed solution, wherein the mass ratio of silk fibroin to polyvinyl alcohol is 5 / 5, and silk fibroin and polyvinyl alcohol The total solute mass concentration of polyvinyl alcohol is 10wt%, and the mixed solution is sucked into the spinning liquid pipe used for electrospinning;

[0044] b. Preparation of reinforced ligament tissue engineering scaffold by electrospinning

[0045] Select a flat metal strip with a width of 10mm and a length of 150mm as the collecting plate, place the spinning liquid tube for electrospinning that has absorbed the composite solution in the electrospinning push pump, and the speed of the electrospinning liquid is 2ml / hour. The high-voltage power supply outputs a voltage of 15kV. The distance between the nozzle of the spinning liquid pipe and the silk thread collection plate is ...

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Abstract

The invention relates to a reinforced ligament tissue engineering scaffold and a preparation method for the same, and belongs to the technical field of medical equipment. The reinforced ligament tissue engineering scaffold consists of a reinforcement skeleton nested between adjacent electrospun nanofiber felt layers, the number of the electrospun nanofiber felt layers is at least three, a material for the electrospun nanofiber felt layers is a composite of silk fibroin and polyvinyl alcohol according to the mass ratio of 1 / 9 to 1 / 1, and the reinforcement skeleton is formed by at least two silk threads or absorbable sutures which are arranged in parallel along the length direction of the electrospun nanofiber felt layers. The scaffold is of a uniform and stable microstructure, and has high biocompatibility, a matrix condition is provided for growth of ligament tissues, and the requirements of keeping slow reduction in mechanical strength of the scaffold in a process of ingrowth of neonatal ligament tissues and slow degradation of nanofibers and stability of a connected joint are met. The preparation method for the scaffold is simple, convenient to operate and adapted to preparation of reinforced ligament tissue engineering scaffolds meeting different mechanical performance requirements.

Description

technical field [0001] The invention relates to a reinforced ligament tissue engineering support composed of a reinforced skeleton nested between adjacent electrospun nanofiber mat layers and a preparation method thereof, belonging to the technical field of medical devices. Background technique [0002] As a tissue engineering ligament scaffold, it needs to meet four conditions, which are biocompatibility suitable for cell adhesion and growth, providing a void structure for cell growth, degradation characteristics compatible with new tissue, and satisfying the structural and mechanical properties of the ligament scaffold. Electrospinning technology is one of the methods for preparing tissue engineering scaffolds. Compared with other methods, this technology is more convenient, simple and flexible, and can be used for most polymers. The fiber membrane prepared by it has the characteristics of large specific surface area, high porosity, high surface energy and high activity, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/16A61L27/50A61L27/36A61L27/58D01D5/00D04H1/728
CPCA61L27/16A61L27/227A61L27/3604A61L27/50A61L27/58A61L2430/10D01D5/0015D01D5/0076D04H1/728C08L89/00C08L29/04
Inventor 柏自奎李佳荣王洪吴星火周应山顾绍金陶咏真杨红军许杰徐卫林
Owner WUHAN TEXTILE UNIV
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