Synchronous monitoring method for chemical signals and electric signals of neural cell network
A technology of nerve cells and electrical signals, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., to meet the monitoring requirements, low background signal, and have the effect of time-space resolution responsiveness
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Embodiment 1
[0033] This embodiment uses an ultra-thin 8×8 microelectrode array with a thickness of 180 μm, the model is 60ThinMEA200 / 30iR-ITO, and the layout is as follows figure 1 shown; figure 1 The middle position shown is the electrode, REF is the reference electrode, surrounded by pins, the first digit of the electrode number is the number of columns, and the second digit is the number of rows; the diameter of a single electrode is 10 μm, the spacing is 30 μm, and the electrode resistance 250 ~ 400kΩ, the electrode material is TiN; the surface of the microelectrode array is provided with a liquid reservoir, the inner diameter is 24mm, and the height is 12mm.
[0034] The detection device of this embodiment is as figure 2 As shown, the microelectrode array 1 is installed on the stage of the total internal reflection fluorescence microscope, so that the microelectrode array 1 is located directly above the total internal reflection objective lens 3; the electrical signal collected by ...
Embodiment 2
[0044] This embodiment adopts the microelectrode array of the same model as that of embodiment 1, and the detection device is the same as that of embodiment 1; the specific steps of using the above microelectrode array in conjunction with a total internal reflection fluorescence microscope device to monitor the chemical signals and electrical signals of the nerve cell network as follows:
[0045] (1) Pretreatment of the microelectrode array: first, the microelectrode array was rinsed in ethanol solution for 20 minutes, then air-dried in the air; finally, 75% ethanol was used for disinfection, and after natural air-drying, it was irradiated with ultraviolet light for 6 hours; Add 0.1 mg / ml polylysine solution to the microelectrode reservoir, and let it stand for 1 hour at 37 ° C in a 5% carbon dioxide environment; then thoroughly clean the microelectrode array with distilled water; finally, use chemical modification method, Put the laminin in the reservoir and let it stand over...
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