Uniseriate chaetomium, microbial agent including same, preparation method and application thereof

A technology of Chaetomium and inoculum, which is applied in the preparation of organic fertilizers, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of application, less Chaetomium in a single row, etc., achieves simple operation, and promotes soil fertility. The effect of raising and increasing organic carbon and soluble carbon and nitrogen content

Active Publication Date: 2020-02-04
HENAN AGRICULTURAL UNIVERSITY
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently there is very little research on Chaetomium monocel...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Uniseriate chaetomium, microbial agent including same, preparation method and application thereof
  • Uniseriate chaetomium, microbial agent including same, preparation method and application thereof
  • Uniseriate chaetomium, microbial agent including same, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] The present invention also provides a preparation method for the bacterial agent described in the above scheme, comprising the following steps:

[0029] 1) inoculating the Chaetomium monolecularis in PD liquid medium, and culturing at 25-30° C. and 120-180 rpm for 7-10 days to obtain the first fermentation mixture;

[0030] 2) inoculating the fermentation mixture on the fermentation medium, and culturing at 25-30°C until the mycelia completely cover the fermentation medium to obtain the first microbial agent;

[0031] The fermentation medium comprises the following ingredients in parts by weight: 65-75 parts of corn stalks, 20-30 parts of bean cake powder, 2-4 parts of sucrose and 90-110 parts of water.

[0032] The present invention inoculates the chaetomium monofilament in PD liquid medium, and cultures it at 25-30°C and 120-180rpm for 7-10 days to obtain the first fermentation mixture; the chaetomium monofilament is preserved in the PDA medium The present invention ...

Embodiment 1

[0043] Embodiment 1 single column Chaetomium F1 is to the plate confrontation experiment of Fusarium

[0044] Inoculate 1 / 4 and 3 / 4 of the diameter of a 90 mm PDA plate with activated Trichomonas and Fusarium, respectively, within 1 week. The Fusarium solani used was Fusarium solani, and the inoculation block diameter was 0.5 cm. Place it in a dark incubator at 25°C for 3 to 4 days, and observe the growth rate of the colony and whether there is an obvious antagonistic effect.

[0045] For test results, see figure 1 ,Depend on figure 1 It can be seen that although the growth rate of Fusarium is slightly faster than that of Trichoraceae, the extension of Fusarium hyphae around Trichoraceae is obviously restricted, and the colony forms a clear gap.

Embodiment 2

[0046] Embodiment 2 A kind of the first bacteria agent that comprises Chaetomium monocele of the present invention

[0047] 1. Inoculate vigorously growing mycelium blocks on PDA medium into PD liquid medium, place on a constant temperature shaker at 28°C and 220rpm for 10 days, and a large number of millet-shaped yellow mycelium balls will appear to obtain a fermentation mixture;

[0048] 2. Under sterile conditions, take 20mL of fermentation mixture and add it to 100g of fermentation medium (straw-soybean cake powder-sucrose medium), shake it up immediately; place it at 28°C for static cultivation; wait until the mycelium solidifies the straw bean cake sucrose medium After agglomeration, put the culture medium horizontally in the Erlenmeyer flask, so that both sides of the mushroom cake can be in contact with the air; then place it in a 28°C incubator until the mycelium completely covers the straw bean cake sucrose culture medium, and smash it with a glass rod, that is, Get ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention discloses uniseriate chaetomium, a microbial agent including the same, a preparation method and application thereof, and belongs to the technical field of microbial agents. The preservation number of the uniseriate chaetomium is CGMCC No.18156. The uniseriate chaetomium promotes improvement of soil fertility by promoting effective degradation of straws, adding a nitrogen content of organic carbon and soluble carbon in soil and resisting to pathogenic bacteria. The uniseriate chaetomium disclosed by the invention comes from farmland soil where straw recycling is carried out for along time and can realize green ecological agriculture, thereby ensuring food quality and safety. The enlarged culture process of the uniseriate chaetomium is simple to operate and low in cost and canprepare microbial agents to be generalized and applied in the agricultural field.

Description

technical field [0001] The invention relates to the technical field of microbial inoculum, in particular to a strain of Chaetomium monocele, the inoculum comprising Chaetomium monocele and its preparation method and application. Background technique [0002] Chaetomium globosa was used as a biocontrol fungus earlier in the field of agriculture. Genome information shows that it has a complete enzyme system for degrading cellulose and hemicellulose, which has important research value in the development of lignocellulose-degrading fungi (Hao Xiaoran , Niu Xueliang, Li Qiang, et al., 2013. Differences in the ability of Chaetomium globosa to degrade natural lignocellulose and enzyme gene analysis, Biotechnology Communications). However, there is very little research on Chaetomyces monolezee at present, and there is no existing technology to apply it to the agricultural field. Contents of the invention [0003] The object of the present invention is to provide a strain of Chaet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N1/14C05F11/00C05F17/00C09K17/14C12R1/645C09K101/00
CPCC05F11/00C05F17/00C09K17/14C09K2101/00C12N1/14C12N1/145C12R2001/645C05F11/08Y02W30/40
Inventor 李芳韩燕来王祎姜瑛
Owner HENAN AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap