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Application of multilineage differentiation and sustained stress cells in preparation of anti-inflammatory drugs

An anti-inflammatory drug and cell technology, applied in the field of stem cell biology and biomedicine, can solve problems such as limited curative effect, side effects, and inability to prevent disease progression

Pending Publication Date: 2020-02-11
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current treatment of inflammation, especially chronic inflammation, still lacks a curative treatment
The existing treatment methods for chronic inflammation mainly include some symptom-relieving drugs, such as non-steroidal anti-inflammatory drugs, steroid hormones, acetaminophen, etc., but these drugs have limited efficacy and cannot prevent the progression of the disease, and long-term It will cause serious side effects after use, therefore, finding and developing new anti-inflammatory drugs has important medical significance and far-reaching social significance

Method used

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  • Application of multilineage differentiation and sustained stress cells in preparation of anti-inflammatory drugs
  • Application of multilineage differentiation and sustained stress cells in preparation of anti-inflammatory drugs
  • Application of multilineage differentiation and sustained stress cells in preparation of anti-inflammatory drugs

Examples

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Effect test

Embodiment 1

[0034] Example 1: Preparation and identification of multilineage differentiated persistent stress cells

[0035]Professional doctors performed bone marrow aspiration on the donor's anterior superior iliac spine, and extracted 1ml of bone marrow. Mix bone marrow and phosphate buffered saline solution at a ratio of 1:1 and add it to the upper layer of the lymphocyte separation medium. After centrifugation, the liquid is divided into four layers. The uppermost layer accounts for about 1 / 2 of the liquid column and is a light red plasma layer; Accounting for 1 / 4 of the liquid column, it is a clear lymphocyte separation liquid layer; at the junction of the two layers is a milky white cloud-like layer with a height of about 5mm, which is a mononuclear cell layer (this layer is the cell layer to be extracted); the most The bottom layer is tightly attached to the test tube wall and is dark red, mainly composed of denser red blood cell layer. Take the second layer of cells, use DMEM / F1...

Embodiment 2

[0037] Example 2: Anti-inflammatory activity detection of multilineage differentiated persistent stress cells

[0038] 1) Mouse inflammation model:

[0039] ①Collagen-induced arthritis model: Under sterile conditions, fully dissolve bovine type II collagen (CII) with 0.05mol / L glacial acetic acid, with a mass concentration of 4mg / mL. After putting it in the refrigerator at 4°C overnight, mix it with Freund's adjuvant (CFA) in equal volume, oscillate and emulsify, take the emulsion and drop it into water if it does not diffuse, indicating that the emulsification is sufficient, and make a CII emulsion (the final concentration of CII is 2mg / mL). The whole process of emulsification was carried out in an ice bath. The mice were continuously anesthetized with isoflurane gas on the operating platform, and after shaved and disinfected, CII emulsion was injected intradermally at the base of the tail and two points on the back of the mice, with a total amount of 100 μL. Two weeks late...

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Abstract

The invention discloses application of multilineage differentiation and sustained stress cells in preparation of anti-inflammatory drugs. The multilineage differentiation and sustained stress cells can be obtained from multiple kinds of mesenchymal tissue, including bone marrow, fat, umbilical cord, dermis and the like. Studies of the invention show that the multilineage differentiation and sustained stress cells are capable of effectively treating multiple kinds of inflammation, such as various diseases and symptoms including arthritis, tendinitis, rhinitis, pneumonia, nephritis, colitis, gynecological inflammation, prostatitis, cellulitis, ulcer, urticaria, tuberculosis, typhoid, autoimmune diseases (such as spondylitis, lupus erythematosus, lymphocytic thyroiditis and the like); and moreover, the multilineage differentiation and sustained stress cells are long in acting time, and have very high clinical application values.

Description

technical field [0001] The invention belongs to the fields of stem cell biology and biomedicine, and specifically relates to the application of multilineage differentiated persistent stress cells in the preparation of anti-inflammatory drugs Background technique [0002] Inflammation refers to the defensive response of living tissues with vascular system to injury factors, such as trauma infection on the body surface and most common and frequently-occurring diseases of various organs (such as arthritis, pneumonia, hepatitis, nephritis, autoimmune inflammation, etc.) ), typical manifestations are red, swollen, hot, and painful, which is a very common and important basic pathological process, and with the development of the disease, inflammation can lead to serious dysfunction, such as articular cartilage rupture caused by arthritis, rheumatoid Joint deformities caused by arthritis, fallopian tube blockage caused by salpingitis, and renal failure caused by chronic nephritis ha...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P29/00
CPCA61K35/28A61P29/00Y02A50/30
Inventor 陈罡顾晓松王晓冬赵雅玉费颖
Owner NANTONG UNIVERSITY
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