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Tremella spore fermentation extract preparation method and product thereof
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A technology of white fungus spores and extracts, which is applied in the field of preparation of white fungus spores fermented extracts for cosmetics, and achieves the effect of high content of active substances
Active Publication Date: 2020-02-28
BLOOMAGE BIOTECHNOLOGY CORP LTD +1
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[0008] Aiming at the problem that there is no preparation method of Tremella spore fermentation extract in the prior art, the present invention provides a preparation method of Tremella spore fermentation extract, by selecting suitable fermentation Viscosity and control of the pH value when removing bacteria make the production cycle short, the content of active substances Tremella polysaccharides and amino acids in the fermentation broth is high, the product is stable, and it is beneficial to later purification
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Embodiment 1
[0034] The preparation of embodiment 1 white fungus spore fermented liquid
[0035] 1. Seed solution preparation
[0036] Tremella fungus strain HX5-1 (preservation number CCTCC M 2017271) was inserted into liquid medium (barley proteinpowder 5 g / L, yeastpowder 5 g / L, glucose 20 g / L, potassium dihydrogen phosphate 3 g / L , magnesiumsulfate 1.5 g / L and V B1 15 mg / L; pH value is 6.0), fermented in a shaking flask at 200 r / min at 30°C for 3 days to obtain seed liquid;
[0037] 2. Three-stage aerated fermentation
[0038] (1) Put the seed liquid into a 10 L fermenter with a 3% inoculation amount, and the culture medium formula is as in step (1), and cultured with aeration and stirring at 30°C, the rotation speed is 200 r / min, and the aeration volume is: initial 10 L / min, 15 L / min after 24 hours, 20 L / min after 48 hours until the end of fermentation, during the fermentation process, use 40% NaOH to adjust the pH value to 6.3-6.5, feed intermittently through feeding, and ferm...
Embodiment 2
[0043] Example 2 Preparation of Tremella spore fermentation extract
[0044] Get the fermented liquid F1 prepared in Example 1, prepare the extract according to the following method:
[0045] (1) Eliminate bacteria: adjust the pH of the fermentation broth to 7.0, and centrifuge at 15,000 rpm for 20 min to obtain the supernatant;
[0046] (2) Decolorization and impurity removal: Adjust the pH of the supernatant to 6.5, add 0.5% w / v activated carbon (200 mesh) for adsorption at 65°C for 1 h, and then filter to obtain filtrate I;
[0047] (3) Protein removal: adjust the pH of filtrate I to 9.5, heat at 90°C for 0.75 h, cool to room temperature and filter to obtain filtrate II;
[0048] (4) Adsorption and impurity removal: Add 0.5% w / v activated carbon (200 mesh) to the filtrate II for adsorption at 60°C for 1 hour, then cool to room temperature and filter through a 0.45 μm polypropylenefilter element to obtain Tremella fungus spore fermentation extract S1.
[0049] According t...
Embodiment 3
[0052] Example 3 Preparation of Tremella spore fermentation extract
[0053] Referring to the method of steps (1)-(4) in Example 2, the fermentation broths F3, F4 and F5 were prepared respectively to prepare Tremella spore fermentation extracts to obtain Tremella spore fermentation extracts S7, S8 and S9.
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Abstract
The invention provides a tremellasporefermentation extract preparation method. The method includes: adjusting pH of fermentation liquor to 7.0-11.0, and centrifuging to obtain supernatant; then, adjusting pH to 5.5-7.0, adding activated carbon for adsorption, filtering to obtain filtrate I; adjusting pH to 8.5-9.5, heating, cooling to the room temperature, and filtering to obtain filtrate II; adding activated carbon into the filtrate II to realize adsorption, and then filtering to obtain tremellasporefermentation extract. By selection of suitable fermentation liquor viscosity and pH valuesin bacterium elimination, easiness in purification of the fermentation liquor can be guaranteed. The fermentation extract is free of stickiness and relatively high in amino acid content, thereby being a natural botanical high-quality skincare and moisturizing product.
Description
technical field [0001] The invention relates to the field of cosmetic raw materials, in particular to a preparation method of tremellaspore fermented extract for cosmetics. Background technique [0002] Tremella is commonly known as white fungus or white fungus. It is recorded in the ancient book of traditional Chinese medicine ("Taiping Jinghui Fang"). Tremella has been used for bath therapy in the Tang Dynasty, which has the effect of moisturizing and smoothing the skin. The appearance of white fungus looks like transparent petals, and it can extract mucopolysaccharides rich in glucose, trehalose, pentose polyacids and mannitol, which have excellent moisturizing power. [0003] The main active ingredient of Tremella fermented extract is Tremella polysaccharide, which is an acidic heteropolysaccharide. The main chain structure is mannan connected by α-(1→3) glycosidic bonds, and the branch chain is composed of glucuronic acid and xylose. Tremella heteroglycans, which have...
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