Dimethomorph hapten, artificial antigen and antibody, preparation method and application thereof
A technology of dimethomorph and artificial antigen, applied in chemical instruments and methods, animal/human proteins, peptides, etc., can solve problems such as inaccurate detection and screening, inability to be widely used, long detection time, etc., and achieve easy Effects of control, good affinity, and mild experimental conditions
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
preparation example Construction
[0034] In the fourth aspect, the present invention also provides a method for preparing the above-mentioned dimethomorph artificial antigen, wherein the preparation of the dimethomorph immune antigen comprises the following steps: dissolving 50 mg of bovine serum albumin with 0.05 mol / L PB buffer, Obtain protein A liquid; dissolve 17mg of dimethomorph hapten in 1mL of N,N-dimethylformamide, add 13.2mg of carbodiimide, mix well, react for 10min, add 9.1mg of N-hydroxysuccinimide , mixed by pipetting, reacted at room temperature for 2 hours, then added dropwise to the above protein A solution, reacted at 4°C for 12 hours, dialyzed and purified with 0.02mol / L PBS buffer for 3 days, subpackaged and stored in freezer;
[0035] The preparation of the dimethomorph-coated antigen comprises the following steps: dissolving 50 mg of ovalbumin with 0.05 mol / L PB buffer to obtain protein B solution; dissolving 9.8 mg of dimethomorph hapten with 1 mL N,N- Dissolve dimethylformamide, add 0.2...
Embodiment 1
[0045] A preparation method of dimethoxyphenyl hapten, the steps are as follows: take 3.18g of 3-(4-chlorophenyl)-3-(3,4-dimethoxyphenyl)-acrylic acid and add 150mL of dichloromethane to dissolve , add 0.6mL of N,N-dimethylformamide, after fully stirring, add 0.13g of oxalyl chloride, stir and react at 0-5°C for 40min, after the reaction is completed, add 1.59g of 3-piperazine-1 propionic acid and triethyl Amine 0.8mL, stirred at room temperature for 3h, after the reaction, add 100mL of water to shake, stand still, remove the water phase, evaporate the organic phase to dryness, put it on a silica gel column, elute and separate with petroleum ether-ethyl acetate with a volume ratio of 1:1 , to obtain the dimethomorph hapten.
Embodiment 2
[0047] A preparation method of dimethomorph immune antigen, the steps are as follows:
[0048] Dissolve 50mg of bovine serum albumin with 0.05mol / L PB buffer to obtain protein A solution; dissolve 17mg of the dimethomorph hapten in Example 1 with 1mL N,N-dimethylformamide, add 13.2mg Carbodiimide, mix well, react for 10min, add 9.1mg N-hydroxysuccinimide, pipette and mix well, react at room temperature for 2h, then add dropwise to the above protein A solution, react at 4°C for 12h, use 0.02mol / L Purify by dialysis against PBS buffer for 3 days, and store in aliquots.
PUM
| Property | Measurement | Unit |
|---|---|---|
| Sensitivity | aaaaa | aaaaa |
| Sensitivity | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More