Cyanine dye FD-1080 J-aggregate as well as preparation method and application thereof

A technology of FD-1080J-, cyanine dye, which is applied in the directions of preparations and pharmaceutical formulations for in vivo experiments, to achieve the effects of excellent spatial resolution, excellent water solubility and biocompatibility, and deep penetration depth

Active Publication Date: 2020-03-06
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, no J-aggregates with maximum absorption and emission wavelengths located above 1300 nm have been reported, and atraumatic imaging of mouse legs, brain vessels, and evaluation of blood pressure reduction through carotid vascular changes have not been reported The efficacy of the drug

Method used

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  • Cyanine dye FD-1080 J-aggregate as well as preparation method and application thereof
  • Cyanine dye FD-1080 J-aggregate as well as preparation method and application thereof
  • Cyanine dye FD-1080 J-aggregate as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh FD-1080 (1.13 mg, 1.5 µmol) and dissolve it in 1 mL of methanol, and dissolve 25 mg of dimyristoylphosphatidylcholine (DMPC) in 1 mL of chloroform. Add 20 µL of FD-1080 stock solution and 407 µL of DMPC stock solution into a 25 mL round-bottomed flask so that the molar ratio of dye to phospholipid is 1:500. After 2 hours, add 2 mL of phosphate buffer solution to a 50°C water bath and sonicate for 10 minutes, then transfer to a probe-type sonication for 15 minutes.

Embodiment 2

[0025] FD-1080 (1.13 mg, 1.5 µmol) was weighed and dissolved in 1 mL methanol, 25 mg dimyristoylphosphatidylcholine (DMPC) was dissolved in 1 mL chloroform, phospholipid polyethylene glycol 2000 (DSPE-PEG 2000 ) 25 mg dissolved in 1 mL chloroform. Add 200 µL FD-1080 stock solution, 155 µL DMPC stock solution, and 30 µL DSPE-PEG to a 25 mL round bottom flask 2000 For the stock solution, the molar ratio of the dye to the phospholipid is 1:20. After spin-drying on a rotary evaporator, pump it in a vacuum oven for 2 hours, add 2 mL of normal saline to a water bath at 50°C and sonicate for 10 minutes before transferring to probe ultrasound for 15 minutes.

Embodiment 3

[0027] Weigh FD-1080 (1.13 mg, 1.5 µmol) and dissolve it in 1 mL of methanol, and dissolve 25 mg of dipalmitoylphosphatidylcholine (DPPC) in 1 mL of chloroform. Add 20 µL of FD-1080 stock solution and 82 µL of DPPC stock solution into a 25 mL round bottom flask so that the molar ratio of dye to phospholipid is 1:100, spin dry on a rotary evaporator, and then pump in a vacuum oven. After 2 hours, add 2 mL of deionized water to a 50°C water bath and sonicate for 5 minutes, then transfer to a probe sonicator for 10 minutes.

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Abstract

The invention belongs to the technical field of biological materials, and particularly relates to a cyanine dye FD-1080 J-aggregate as well as a preparation method and a biological application thereof. The preparation method comprises the steps that cyanine dye and phospholipid are self-assembled through a thin film hydration method to form an aggregate solution, and the cyanine dye is organic small molecule heptamethine cyanine fluorescent dye and is marked as a fluorescent probe FD-1080. The maximum absorption and emission peaks of the prepared J-aggregate are both greater than 1300 nm, andthe red shift of the J-aggregate is 300 nm or above compared with that of a monomer of the J-aggregate; the J-aggregate is uniform in size and has good water solubility and biocompatibility; under excitation of a 1064 nm laser, non-invasive brain and leg blood vessel fluorescence imaging of 1500 nm or above can be achieved, and high spatial resolution and signal-to-noise ratio are achieved. By utilizing the J aggregate, real-time dynamic vascular imaging of carotid artery of a hypertensive rat can be realized, and a clinically applied antihypertensive drug can be rapidly evaluated.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a cyanine dye FD-1080 J-aggregate, a preparation method and biological application thereof. Background technique [0002] Accurate biomedical imaging methods are crucial for the diagnosis and prognosis of diseases. Among them, fluorescence imaging has shown superior performance in terms of high sensitivity, high temporal resolution, and fast feedback, but is limited to a low tissue penetration depth. In recent years, because the second near-infrared window (1000-1700 nm) has lower biological tissue light scattering, smaller absorption and autofluorescence compared with the first near-infrared window (700-900 nm), researchers have attracted much attention. of widespread attention. Especially for the window above 1500 nm, due to the weak absorption and scattering of biological tissues, the imaging quality and penetration depth can be greatly improved. Th...

Claims

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Application Information

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IPC IPC(8): A61K49/00
CPCA61K49/0032
Inventor 张凡孙彩侠李本浩赵梦瑶
Owner FUDAN UNIV
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