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Photonic crystal heavy metal sensor with immobilized enzyme and preparation method thereof

A photonic crystal, immobilized enzyme technology, applied in immobilized enzymes, biochemical equipment and methods, immobilized on/in organic carriers, etc. The pre-processing steps are cumbersome and expensive, so as to achieve the effect of being beneficial to the identification and response, reducing the detection cost, and being easy to operate.

Active Publication Date: 2020-03-06
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods generally have high sensitivity and accuracy, but at the same time, there are great limitations: the analysis methods relying on large instruments are relatively expensive, the pretreatment steps are cumbersome and time-consuming, and special equipment and operators are required , it is difficult to meet the requirements of miniaturization, real-time and rapid on-site detection

Method used

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  • Photonic crystal heavy metal sensor with immobilized enzyme and preparation method thereof
  • Photonic crystal heavy metal sensor with immobilized enzyme and preparation method thereof
  • Photonic crystal heavy metal sensor with immobilized enzyme and preparation method thereof

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preparation example Construction

[0029] A photonic crystal heavy metal sensor with immobilized enzyme and a preparation method thereof, the specific steps are:

[0030] Step 1: Prepare ethanol prepolymer solution containing functional monomer, crosslinking agent and initiator, the volume ratio of functional monomer, crosslinking agent, solvent and photoinitiator is (1-5):(0.1-1): (5-15): (0.1-0.5), then shake the pre-poly solution to dissolve, and put it in the refrigerator overnight.

[0031] Step 2: Infiltrate the pre-polymerization solution obtained in Step 1 from the edge of the photonic crystal. When the photonic crystal is transparent, cover the photonic crystal with a plexiglass sheet, and then put it under the ultraviolet light for 3-6 hours. Finally, it is taken out and etched with hydrofluoric acid. The concentration of hydrofluoric acid is 1%-4%, and the polymer photonic crystal is obtained, which is put into water to balance it for later use.

[0032] Step 3: Weigh dopamine and add it to a tris(h...

Embodiment 1

[0037] Step 1: Prepare a pre-polymerization solution containing methacrylic acid, ethylene glycol dimethacrylate, ethanol, and dihydroxydimethylpropiophenone at a volume ratio of 1: 0.1: 5: 0.1, and then shake the pre-polymerization solution to dissolve , and refrigerate overnight.

[0038]Step 2: Infiltrate the pre-polymerization solution obtained in Step 1 from the edge of the photonic crystal. When the photonic crystal is transparent, cover the photonic crystal with a plexiglass sheet, then put it into the ultraviolet light for 4 hours, and finally put the It is taken out and etched with hydrofluoric acid to obtain a polymer photonic crystal, which is put into water to balance it for standby.

[0039] Step 3: Weigh 120 mg of dopamine and add it to tris(hydroxymethylamino)methane buffer solution with a pH of 8.5 to prepare a polydopamine solution with a concentration of 20 mg / mL, and then add the photonic crystal with polymer obtained in step 2 Insert it into the polydopami...

Embodiment 2

[0048] Step 1: Prepare a pre-polymerization solution containing acrylic acid, ethylene glycol dimethacrylate, ethanol, and dihydroxydimethylpropiophenone at a volume ratio of 1: 0.5: 5: 0.1, then shake the pre-polymerization solution to dissolve, and put Chill overnight in the refrigerator.

[0049] Step 2: Infiltrate the pre-polymerization solution obtained in Step 1 from the edge of the photonic crystal. When the photonic crystal is transparent, cover the photonic crystal with a plexiglass sheet, then put it into the ultraviolet light for 4 hours, and finally put the It is taken out and etched with hydrofluoric acid to obtain a polymer photonic crystal, which is put into water to balance it for standby.

[0050] Step 3: Weigh 120 mg of dopamine and add it to tris(hydroxymethylamino)methane buffer solution with a pH of 8.5 to prepare a polydopamine solution with a concentration of 20 mg / mL, and then add the photonic crystal with polymer obtained in step 2 Insert it into poly...

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Abstract

The invention discloses a photonic crystal heavy metal sensor with immobilized enzyme and a preparation method of the photonic crystal heavy metal sensor. The preparation method comprises the following specific steps of firstly, preparing a pre-polymerization solution containing a functional monomer, a cross-linking agent, ethanol and a photoinitiator, pouring the pre-polymerization solution intogaps of photonic crystal colloidal particles, and putting the photonic crystal colloidal particles under an ultraviolet lamp for polymerization; etching with hydrofluoric acid to obtain an inverse protein structure photonic crystal sensor with pH responsiveness after the polymerization is finished; inserting one side, without a photonic crystal organic glass sheet, of the photonic crystal sensor into a polydopamine solution for placement; and taking out the photonic crystal sensor and inserting one side with a polydopamine coating into a urease solution with a certain concentration to immobilize urease to obtain the photonic crystal heavy metal sensor with the immobilized enzyme. Compared with a traditional detection method, an inverse opal structure photonic crystal sensor with the immobilized enzyme has the advantages of simpler and more convenient operation, better stability, higher sensitivity and higher response speed.

Description

technical field [0001] The invention belongs to the technical field of nano functional material sensors, and relates to a photonic crystal heavy metal sensor with immobilized enzyme and a preparation method thereof. Background technique [0002] With the development of society and economy, my country has made remarkable achievements in the industrial field, greatly improving people's lives, but at the same time, the negative impact brought about by the growth of the industrial system cannot be ignored, such as the discharge of industrial wastewater and the disposal of industrial waste. Arbitrary landfilling seriously affects people's life and health. Among them, the harm of heavy metal pollution to people is one of the more common and serious pollution. There is no strict definition of heavy metals at home and abroad, but in the field of chemistry, it generally refers to the relative specific gravity greater than or equal to 4.5g / cm 3 A class of metal elements, including me...

Claims

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Application Information

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IPC IPC(8): G01N21/78C12N11/089
CPCG01N21/78C12N11/08C12N9/80C12Y305/01005
Inventor 李露董徐刚李静茹孟甜甜
Owner SHAANXI UNIV OF SCI & TECH