Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Laser desorption ionization mass spectrometry method and system

An ionization mass spectrometry and analysis method technology, which is applied in the field of laser desorption ionization mass spectrometry analysis, can solve problems that have not been reported yet, and achieve avoidance of inhomogeneous co-crystallization, sensitive mass spectrometry soft ionization detection and mass spectrometry imaging analysis, and simplified operation steps Effect

Inactive Publication Date: 2020-03-06
TSINGHUA UNIV
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, no method has been reported to directly modify polydopamine onto solid materials for laser desorption ionization mass spectrometry analysis.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Laser desorption ionization mass spectrometry method and system
  • Laser desorption ionization mass spectrometry method and system
  • Laser desorption ionization mass spectrometry method and system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Using polydopamine-modified stainless steel sheets and polydopamine-modified laser-etched stainless steel sheets as sample-loading substrates for laser desorption ionization mass spectrometry analysis of different analytes

[0037] Preparation of analytical samples: Glucose solution with a concentration of 1mg / ml, the solvent is water; phosphatidylcholine PC 16:0 / 18:1 solution with a concentration of 10μM, the solvent is methanol / water 1 / 1; the concentration is 0.2mg / ml of angiotensin II solution, the solvent is methanol / water 3 / 7; configure polyethylene glycol PEG 600 (0.1μL / L), PEG 1000 (15μL / L), PEG 2000 (20μL / L) mixture solution , placed in a 4°C refrigerator for later use.

[0038] Laser etching stainless steel sheet: the manufacturing method is as patent application CN103627883, made on stainless steel (304).

[0039] Polydopamine modification: prepare a dopamine solution with a concentration of 2 mg / ml, the solvent is a 10 mM aqueous solution of trish...

Embodiment 2

[0046] Example 2 Laser desorption ionization mass spectrometry analysis using polydopamine-modified stainless steel sheet as a sample-loading substrate

[0047] Preparation of analytical samples: prepare a glucose solution with a concentration of 1 mg / ml, use water as a solvent, and place it in a refrigerator at 4°C for use.

[0048] Polydopamine-modified stainless steel sheet: prepare a dopamine solution with a concentration of 0.25 mg / ml, and a solvent of 10 mM tris aqueous solution (pH 8-9), place it in an open beaker and stir; wait for the resulting solution to change color Finally, the solid material to be modified was directly put into it to soak and stirred continuously at a speed of 300 rpm, reacted at room temperature for 15 hours, and then taken out. The obtained target plate was thoroughly washed with deionized water to remove surface impurities and dopamine monomers, and then dried in an oven.

[0049] LDI mass spectrometry analysis:

[0050] (1) The polydopamine...

Embodiment 3

[0052] Example 3 Laser desorption ionization mass spectrometry analysis of human plasma and plasma extracts using polydopamine-modified laser-etched stainless steel sheet as a sample-loading substrate

[0053] Preparation of plasma extract: 50 μL of human plasma was added to 1 mL of deionized water, and then 1 mL of methanol and 2 mL of chloroform were added. After vortexing for 5 minutes, place in a centrifuge and centrifuge for 8 minutes (11269×g), collect the chloroform phase, blow dry with nitrogen, then redissolve the precipitate in 1 mL of methanol, and store in a -20°C refrigerator for later use.

[0054] Laser etching stainless steel sheet: the manufacturing method is as patent application CN103627883, made on stainless steel (304).

[0055] Polydopamine modification: prepare a dopamine solution with a concentration of 2 mg / ml, the solvent is a 10 mM aqueous solution of trishydroxymethylaminomethane (pH is 8 to 9), place it in an open beaker and stir; after the resulti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Concentrationaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses a laser desorption ionization mass spectrometry method. The method comprises the steps of: (1) forming a polydopamine modified layer on the surface of a solid material so as toobtain a sample carrying substrate; (2) applying a to-be-detected sample to the surface of the polydopamine modified layer of the sample carrying substrate, and then drying the to-be-detected sample;and (3) carrying out laser desorption ionization mass spectrometry on the sample carrying substrate. The polydopamine modified solid material is used as the sample carrying substrate (a target plate)for laser desorption ionization, only the one-step target plate preparing process is required, and a matrix does not need to be added; by adopting the polydopamine modified solid material to carry out spectrometry detection and spectrometry imaging, the laser desorption ionization mass spectrometry method has an excellent signal-to-noise ratio, excellent repeatability and a high spatial resolution, and can implement universal, simple, efficient, rapid and sensitive spectrometry soft ionization detection and spectrometry imaging analysis.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry. Specifically, the present invention relates to laser desorption ionization mass spectrometry methods and systems. Background technique [0002] Mass spectrometry imaging (MSI) is a molecular imaging technology based on mass spectrometry. By scanning mass spectrometry on the surface of biological tissue slices, the spatial distribution and Abundance information is of great significance for drug metabolism distribution research, pathology research, and discovery of disease biomarkers. Matrix-assisted laser desorption / ionization (MALDI) technology is currently the most widely used mass spectrometry imaging technology, and has been widely used in mass spectrometry imaging analysis of molecules such as peptides, proteins, drugs and lipids in recent years. In the traditional MALDI imaging analysis process, the basic steps are: prepare tissue slices and transfer them to the MALDI target plat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/62
CPCG01N27/628
Inventor 欧阳证杨静
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com