Whole-cell biosensor for detecting heavy metal ions in water-soluble sample and construction and application thereof

A heavy metal ion, biosensor technology, applied in the field of environmental biology, can solve the problems of not easy to carry instruments, expensive instruments, long detection period and so on
CN110873790AActive Publication Date: 2020-03-10SOUTH CHINA UNIV OF TECH +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SOUTH CHINA UNIV OF TECH
Publication Date
2020-03-10

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Abstract

The invention discloses a whole-cell biosensor for detecting heavy metal ions in a water-soluble sample and construction and application thereof. The operation object is escherichia coli, no pathogenic risk exists, and the operation is simple and easy to implement. An SRRz cracking gene is used as a report element, so that microbial bacteria are cracked, and the turbidity of bacterial liquid is obviously changed. Detection can be performed through a visible spectrophotometer, and a result can be obtained through direct observation with naked eyes. The response is quick, and only 30-60 minutesare needed from sample contact to result acquisition. X-gal is used for detecting beta-galactosidase released to the outside of the cells, and the operation is simple and convenient. Expensive instruments are not needed in application, so that the detection cost is greatly reduced, and field detection becomes possible. The extracellular beta-galactosidase activity is accurately quantified by usingchromogenic gel containing oNPG, the detection limit is low, and the detection result is accurate. Technical support is provided for daily heavy metal pollution detection and sudden heavy metal pollution detection.
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Description

technical field

[0001] The invention belongs to the technical field of environmental biology, and particularly relates to a whole-cell biosensor for highly sensitive detection of heavy metal ions (divalent mercury ions or lead ions) in water-soluble samples, and its construction and application. Background technique

[0002] With the development of industry, heavy metal pollution has become increasingly serious and has become one of the prominent environmental problems. Heavy metals are highly toxic, easy to accumulate in organisms and difficult to degrade, and bring great threats to human health after entering the food chain. Therefore, the detection technology of heavy metal ions is particularly important. The detection techniques of heavy metal ions mainly include atomic absorption spectrometry, atomic fluorescence spectrometry, inductively coupled plasma method, ultraviolet-visible spectrophotometry, high performance liquid chromatography, electrochemical analysis, biolo...

Claims

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