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Preparation and application of porous trimanganese tetroxide nanoprobe

A technology of manganese tetraoxide nanometer and nanoprobe, applied in nanotechnology, nano-optics, nanotechnology, etc., can solve the problems of short free radical life, easy removal by GSH, poor photodynamic therapy effect, etc., to improve oxygen content, treatment efficiency enhancement, and the effect of increasing photodynamic efficiency

Active Publication Date: 2020-03-13
HUAZHONG UNIV OF SCI & TECH
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Problems solved by technology

[0005] In view of the above defects or improvement needs of the prior art, the present invention provides the preparation and application of a porous manganese tetraoxide nanoprobe, which is prepared by the one-pot method of the manganese metal-organic framework nanomaterial Mn- MOF is calcined at high temperature to obtain porous trimanganese tetraoxide nanomaterials, which are used to prepare probes for photodynamic therapy of tumors into the nucleus, thereby solving the problem of free photoreaction of photodynamic therapy probes in the prior art. Short base lifetime and easy to be cleared by GSH lead to technical problems of poor photodynamic therapy effect

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  • Preparation and application of porous trimanganese tetroxide nanoprobe
  • Preparation and application of porous trimanganese tetroxide nanoprobe

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[0033] The invention provides a preparation method of porous trimanganese tetraoxide nanomaterials, comprising the following steps:

[0034] (1) Synthesis of Mn-MOF, a nanomaterial of manganese metal-organic framework, by a one-pot method;

[0035] (2) Calcining the manganese metal-organic framework nanomaterial Mn-MOF obtained in step (1) to obtain porous trimanganese tetraoxide nanomaterials.

[0036] In some embodiments, step (1) is specifically: manganese chloride tetrahydrate (MnCl 2 4H 2 The manganese ion of 0) is a metal salt, and 2-Methylimidazole (2-mIm) is used as an organic framework to synthesize a metal-organic framework nanomaterial Mn-MOF by a one-pot method.

[0037] The one-pot method of the present invention is to mix and stir the aqueous solution of manganese chloride tetrahydrate and the aqueous solution of the organic framework to prepare the Mn-MOF. In some embodiments, the dosage range of manganese chloride tetrahydrate and organic framework can be ad...

Embodiment 1

[0067] A porous Mn 3 o 4 The preparation method of nanoprobe comprises the following steps:

[0068] (1) Using manganese ions of manganese chloride tetrahydrate as metal ions and 2-methylimidazole as the organic framework, Mn-MOF was synthesized by a one-pot method.

[0069] (2) Calcining the Mn-MOF in (1) at high temperature to obtain Mn 3 o 4 .

[0070] (3) the Mn in (2) 3 o 4 After reaction with MPTMS and TCEP, modification of polyethylene glycol with maleimide yielded Mn 3 o 4 -PEG.

[0071] (4) On the basis of (3), load the photosensitive molecule Ce6 and the nuclear sequence NLS.

[0072] Specifically, in step (1), the metal-organic framework of manganese is prepared by the following method:

[0073] 1) Prepare the solutions of manganese chloride tetrahydrate and 2-methylimidazole respectively for use: 1.55 mmol of manganese chloride tetrahydrate is dissolved in 3 mL of ultrapure water and ultrasonically dispersed to obtain an aqueous solution of cerium nitrate...

Embodiment 2

[0079] This embodiment provides a method for synthesizing porous trimanganese tetraoxide nanoprobes, the material is distributed in a cube, and the longest side length is 40nm-50nm;

[0080] The material is prepared by the following preparation method:

[0081] (1) Preparation of MOF

[0082] In the vigorously stirred 20mL water reaction system containing 5.5g 2-mIm, add 3mL of manganese chloride tetrahydrate solution with 1.55mmol dissolved therein dropwise, and continue to stir at room temperature for 6h, the color changes from colorless to dark brown, through 6000rpm, The unreacted metal ions and imidazole were washed away by centrifugation for 10 min to obtain a dark brown precipitate, which was MOF.

[0083] Such as figure 1 Shown is the transmission electron microscope topography of the obtained MOF. The longest side length of the obtained MOF is 50nm, and has good dispersion.

[0084] (2) Mn 3 o 4 preparation of

[0085] Place the MOF powder in step (1) in a muffl...

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Abstract

The invention belongs to the technical field of nano-biomaterials, and more specifically relates to preparation and application of a porous trimanganese tetroxide nanoprobe. According to the invention, a manganese metal-organic framework nano-material Mn-MOF prepared by a one-pot method is calcined at high temperature to obtain a porous trimanganese tetroxide nano-material. When Mn3O4 is used as acarrier for cancer treatment, the surrounding hydrogen peroxide can be catalyzed into oxygen, thus improving the oxygen content in tumor cells and further increasing the photodynamic efficiency. Meanwhile, Mn3O4 also has the ability to consume GSH, and can consume GSH in cells during cancer treatment to prevent GSH from scavenging free radicals in photodynamic reactions, which also greatly increases the photodynamic efficiency. After modifying a series of nuclear targeting sequences, the nano-material is endowed with the nature of nuclear import, thus realizing nuclear import photodynamic therapy with the enhanced therapeutic efficiency and effectively killing tumor cells.

Description

technical field [0001] The invention belongs to the technical field of nano-biological materials, and more specifically relates to the preparation and application of a porous manganese tetraoxide nano-probe. Background technique [0002] Compared with traditional surgery, radiotherapy and chemotherapy, photodynamic therapy has always been a very popular tumor treatment method due to its advantages of high selectivity, less damage to normal tissues, low toxicity and repeatable treatment. [0003] Photodynamics is mainly composed of three factors: photosensitive molecules, oxygen, and laser light sources with specific wavenumbers. The effect of photodynamic therapy depends on the efficiency of photodynamic therapy. A high-efficiency photoreaction can generate a large amount of active oxygen, thereby achieving a good photodynamic therapy. Many factors affect the effect of photodynamics, such as the power of the laser light source, the content of ambient oxygen, the concentrati...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K33/32A61K47/60A61P35/00B82Y20/00B82Y30/00B82Y40/00
CPCA61K33/32A61K41/0052A61K47/60A61P35/00B82Y20/00B82Y30/00B82Y40/00
Inventor 刘笔锋曾雪梅
Owner HUAZHONG UNIV OF SCI & TECH
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