Bactericidal active water, preparation method thereof and application thereof

A bactericidal activity and inactivation technology, applied in the field of food microorganisms, can solve the problems of residual environmental pollution, failure to keep fresh, supporting power equipment and testing equipment, etc., to achieve high processing efficiency, improve efficiency, and avoid decay and deterioration.

Inactive Publication Date: 2020-04-07
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Freezing and modified atmosphere fresh-keeping methods require a large investment, and there are many supporting power equipment and testing equipment, which have the disadvantages of being difficult to inhibit the growth of mold and eliminate residual chemicals, and at the same time cause the water loss of vegetables and fruits and fail to achieve the purpose of fresh-keeping
Chemical preservatives are mainly sprayed and fumigated with chemical agents such as formaldehyde, sulfite, and sodium hypochlorite, and there are serious problems of drug residues and environmental pollution
The radiation preservation method requires a large investment, and the radiation technology is not easy to be strictly controlled and popularized. Once the dose is excessive, it will cause a certain degree of radioactive pollution and cause harm to the human body, so its application is limited.

Method used

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  • Bactericidal active water, preparation method thereof and application thereof
  • Bactericidal active water, preparation method thereof and application thereof
  • Bactericidal active water, preparation method thereof and application thereof

Examples

Experimental program
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Effect test

Embodiment

[0033] 1. Preparation of bacterial suspension

[0034] The standard strain of Saccharomyces cerevisiae (ATCC9763) was cultured and passaged with fresh YPD solid medium. Use a sterile inoculation loop to pick a vigorous single colony and inoculate it in YPD liquid medium, culture it on a shaker at 30 °C and 120 r / min for 12 h until the logarithmic growth phase, collect the bacteria by centrifugation and resuspend in sterile physiological brine to 10 8 CFU / mL, spare.

[0035] 2. Grape preparation and inoculation

[0036]1. Sample preparation: Select grapes with intact surface, similar color and size, and weigh them to ensure that the weight is about 7 g.

[0037] 2. Inoculation: Put the prepared grape samples into a beaker with 200 mL of bacterial suspension and soak for 30 min. It is obtained through experiments that the concentration of bacteria attached to the grape surface at this time is 10 6 CFU / g.

[0038] 3. Bactericidal active water synergy with Ɛ-polylysine and...

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Abstract

The invention belongs to the field of food microorganisms and relates to bactericidal active water, a preparation method thereof and application thereof. The bactericidal active water is plasma activewater (PAW) obtained after deionized water subjected to high-pressure sterilization is treated by a low-temperature plasma surface treatment machine. The treatment conditions of the low-temperature plasma surface treatment machine are as follows: the frequency is 18-60 KHz, the power is 750 W, the working air pressure is 0.15-0.4 MPa, the treatment time is 60-100 seconds, and the temperature is room temperature. After PAW and polylysine (epsilon-PL) synergistic treatment at 50 DEG C, serious irreversible damage occurs to the cell structure of saccharomyces cerevisiae, so that the normal physiological function of cells is destroyed, and finally cell death is caused, which may be the main reason for saccharomyces cerevisiae death caused by PAW and polylysine warm synergistic treatment.

Description

technical field [0001] The invention belongs to the field of food microorganisms, and relates to a bactericidal active water and a preparation method and application thereof. Background technique [0002] Fruits and vegetables, short for fruits and vegetables, refer to edible fruits and vegetables. Fruits and vegetables are rich in active functional ingredients such as vitamins, inorganic salts, biological enzymes and plant fibers necessary for human life and health, which are very beneficial to human health. However, food and agricultural products are susceptible to microbial contamination during growth and processing, which eventually leads to food corruption and deterioration. With the improvement of people's living standards, people pay more and more attention to food safety and food quality. [0003] At this stage, the cleaning situation of equipment in food processing is not optimistic. There are two main reasons for this situation. One is that the cleaning process o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F1/30A23B7/154A23L5/20C02F103/02
CPCC02F1/30A23B7/154A23L5/27C02F2103/02
Inventor 相启森张嵘马云芳刘骁牛力源白艳红范刘敏董闪闪李云菲
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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