Preparation method of germinated tartary buckwheat powder
A technology for tartary buckwheat powder and tartary buckwheat is applied in the directions of botanical equipment and methods, seed and rhizome treatment, nitrogen fertilizer, etc., which can solve the problems of low flavonoid content and achieve the effect of improving flavonoid content, good effect and inhibiting amylase activity.
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Embodiment 1
[0026] A preparation method for sprouting tartary buckwheat flour, comprising the following steps:
[0027] S1: Disinfection: Choose tartary buckwheat seeds that are not damaged, uniform in particle size, and mature and plump as raw materials; wash and dry the tartary buckwheat seeds, and soak them in a 5% sodium hypochlorite solution for 10 to 15 minutes;
[0028] S2: Irradiation and pressurization: put the sterilized tartary buckwheat seeds in a microwave oven and irradiate them with a microwave power of 600W for 10s, and then put them under a pressure of 80Mpa for 3min;
[0029] S3: Wheat soaking: add the irradiated and pressurized tartary buckwheat seeds to the nutrient solution (5mmol / L L-phenylalanine solution, 160mmol / L NaCl solution, 200mg / L zinc sulfate solution and 30mg / L gibberellin solution) for 3 hours, placed in a constant temperature and humidity incubator for 5 times of soaking treatment after taking it out, and the temperature of the constant temperature was s...
Embodiment 2
[0034] A preparation method for sprouting tartary buckwheat flour, comprising the following steps:
[0035] S1: Disinfection: Choose tartary buckwheat seeds that are not damaged, uniform in particle size, and mature and plump as raw materials; wash and dry the tartary buckwheat seeds, and soak them in 5% sodium hypochlorite solution for 10-15 minutes;
[0036] S2: Irradiation and pressurization: put the sterilized tartary buckwheat seeds in a microwave oven and irradiate them with a microwave power of 600W for 10s, and then put them under a pressure of 80Mpa for 3min;
[0037] S3: Wheat soaking: add the irradiated and pressurized tartary buckwheat seeds to the nutrient solution (5mmol / L L-phenylalanine solution, 160mmol / L NaCl solution, 200mg / L zinc sulfate solution and 30mg / L gibberellin solution) for 3 hours, placed in a constant temperature and humidity incubator for 5 times of soaking treatment after taking it out, and the temperature of the constant temperature was set at...
Embodiment 3
[0042] (1) The making of total flavonoid content standard curve
[0043] Pipette 1, 2, 4, 6, 8mL of 0.264g / L rutin standard solution into a 25mL volumetric flask, add 12.5mL of 30% ethanol, add 0.7mL of 48g / L sodium nitrite solution, and then add 91g / L rutin solution after 5min. 0.7 mL of L aluminum nitrate solution, after 6 min, add 5 mL of 1 mol / L sodium hydroxide solution, and finally dilute to volume with 30% ethanol solution, and place for 10 min. Take the sample without rutin standard solution as a blank, and measure its absorbance at a wavelength of 510nm. Taking the mass concentration of rutin as the abscissa and the absorbance as the ordinate, draw a standard curve to draw a regression equation, and its standard curve is as follows figure 1 shown. Depend on figure 1 It can be seen that the regression equation of rutin mass concentration and absorbance is y=9.6252x+0.0006, and the linear correlation coefficient R 2 = 0.9998.
[0044] (2) Determination of flavonoid...
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