The invention discloses a method for preparing high-purity sulforaphene from carmine radish seeds, and relates to a method and a technology for rapidly and steadily preparing sulforaphene from the carmine radish seeds. According to the method, the powder of skimmed carmine radish seeds sold in the market is taken as a raw material; through enzymolysis and centrifugation, a centrifugate is obtained; resin coarse separation and high performance liquid chromatography preparation separation are sequentially conducted on the obtained centrifugate, so that a sulforaphene preparation liquid is obtained; finally, through rapid alcohol-water displacement, concentration, and freeze-drying, a sulforaphene product of which the purity reaches 99% or higher is obtained. The method is simple to operate, efficient, and high in continuity, and has the advantages that solvents can be repeatedly recovered and utilized, resin can be recycled, the sulforaphene product is steady and high in purity, and the problems that sulforaphene is liable to be subjected to rearrangement or isomerization in water, and the sulforaphene product is difficult to preserve for a long time can be solved. The sulforaphene product prepared according to the method can be widely applied to the fields of health products and pharmaceuticals.