Multifunctional enterococcus faecium CCFM1106 and feed additive thereof, and applications of multifunctional enterococcus faecium CCFM1106 and feed additive

A CCFM1106, feed additive technology, applied in the field of microorganisms, can solve the problems of high cost, high price, difficult to obtain, etc., and achieve the effects of reducing content, improving liver enlargement, and reducing adsorption capacity

Active Publication Date: 2020-05-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the methods to alleviate the toxicity of PFOS start with natural chemical substances with high antioxidant activity. In the research, such as quercetin and lycopene, which have a mitigating effect, are expensive and difficult to obtain. For animals such as poultry and livestock say the cost is too high

Method used

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  • Multifunctional enterococcus faecium CCFM1106 and feed additive thereof, and applications of multifunctional enterococcus faecium CCFM1106 and feed additive
  • Multifunctional enterococcus faecium CCFM1106 and feed additive thereof, and applications of multifunctional enterococcus faecium CCFM1106 and feed additive
  • Multifunctional enterococcus faecium CCFM1106 and feed additive thereof, and applications of multifunctional enterococcus faecium CCFM1106 and feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Example 1: Enterococcus faecium CCFM1106 has good PFOS adsorption capacity

[0066] Bacterial adsorption Enterococcus faecium CCFM1106 was purified and activated, inoculated in MRS liquid medium at 1% (v / v) inoculum, and cultured at 37°C for 12 hours. Then centrifuge at 8000r / min for 5min to collect the bacteria, take the precipitate and clean it with normal saline, then continue to centrifuge at 8000r / min for 5min to remove the precipitate to obtain live bacteria cells, that is, wet bacteria. The wet bacteria were resuspended in 20mg / L PFOS solution, and the final bacterial cell concentration reached 1g dry cells / L (wet bacteria were resuspended in PFOS-free ultrapure water as a blank control). Use 0.1M NaOH or HCl solution to quickly adjust the pH of the PFOS solution containing bacteria to 3.0, add a small amount of NaOH or HCl (less than 0.5ml), and its ionic strength has negligible effect on PFOS adsorption. Subsequently, the 250ml Erlenmeyer flask containing 100m...

Embodiment 2

[0071] Embodiment 2: Enterococcus faecium CCFM1106 has a good ability to scavenge diphenyltrinitrophenylhydrazine free radicals (DPPH) in vitro, scavenge hydroxyl free radicals and reducing ability;

[0072] After fully mixing 1 mL of Enterococcus faecium CCFM1106 complete cell suspension with 1 mL of freshly prepared DPPH absolute ethanol solution (0.2 mmol / L), they were reacted in the dark at 37°C for 30 min. Mix DPPH and PBS (pH7.2) as a control sample, and culture under the same conditions. After centrifuging at 7000×g for 10 min, measure the absorbance at 517 nm, and calculate the ability of lactic acid bacteria to scavenge DPPH free radicals according to the following formula:

[0073] DPPH radical scavenging rate (%)=[1-A517 (sample) / A517 (control)]×100%.

[0074] Mix 1 mL of 1,10-phenanthroline, 1 mL of LPBS (pH 7.2), 1 mL of Enterococcus faecium CCFM1106 complete cell suspension or 1 mL of FeSO4 (referred to as "mixture 1"). Add 1 mL of H to "Mixture 1" 2 o 2 , in...

Embodiment 3

[0077] Example 3: Enterococcus faecium CCFM1106 can significantly improve liver enlargement in PFOS-exposed mice

[0078] Twenty-four 6-week-old male C57BL / 6J mice were randomly divided into four groups according to body weight after one week of acclimatization: blank control group, model group, quercetin intervention group, and Enterococcus faecium CCFM1106 intervention group, with 6 mice in each group See Table 2 for mice, animal groups and treatment methods.

[0079] Table 2 Grouping and processing methods of animal experiments

[0080]

[0081] The mice in Example 2 were weighed on the 31st day, then euthanized, blood was taken from the orbit, the liver was taken out and weighed wet to calculate the organ coefficient, and the organ coefficient of the mouse liver was calculated according to the following formula:

[0082] Liver organ coefficient = liver wet weight (g) / mouse body weight before euthanasia (100g)

[0083] Experimental results such as image 3 As shown, t...

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Abstract

The invention discloses multifunctional enterococcus faecium CCFM1106 and a feed additive thereof, and applications of the multifunctional enterococcus faecium CCFM1106 and the feed additive. The enterococcus faecium CCFM1106 has good adsorption capacity to PFOS in vitro to reduce the concentration of PFOS, has good diphenyl trinitrophenylhydrazine free radical and hydroxyl radical removal capacity and reducing capability, improves liver swelling of mice caused by PFOS exposure, improves levels of CAT, SOD and antioxidant capacity, improves intestinal flora disorder, reduces abundance of S24-7family, lactobacillus, bifidobacterium and bacteroides in intestinal tracts of the mice, and reduces occurrence tendency of diseases like liver diseases, hypertension, diabetes and obesity. The enterococcus faecium CCFM1106 can be used for preparing feed additives and medicines capable of relieving PFOS toxicity, and has extensive application prospect.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to multifunctional Enterococcus faecium CCFM1106, its feed additive and its application. Background technique [0002] Perfluorinated compounds have been widely used in various industries due to their hydrophobic and oleophobic properties, as well as good thermal stability, chemical stability and biological stability. Used in ready-to-wear (such as waterproof, anti-fouling outdoor clothing) and home textiles (such as carpets, furniture fabrics, etc.), feed additives, personal care products (such as dental floss) and fire extinguishing foam, etc. Among them, PFOS, as one of the final transformation products of various fluorine compounds, can directly cause harm to humans and animals through environmental exposure, or enter animals and plants and bioaccumulate in the human body through the food chain, so it has been attracted by research scholars. more and more attention...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K10/18C12R1/01
CPCA23K10/18C12N1/20C12N1/205C12R2001/46
Inventor 陈卫王刚孙姗姗朱慧越赵建新张灏
Owner JIANGNAN UNIV
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