Supercharge Your Innovation With Domain-Expert AI Agents!

Enterobacterroggenkampii and application thereof in degradation of ethylene oxide

A technology of Enterobacter rogerii and ethylene oxide, applied in the direction of bacteria, methods based on microorganisms, methods using microorganisms, etc., can solve the problems of easy explosion, increased ethylene oxide barrier, and high technical parameter control requirements , to achieve the effect of outstanding tolerance and significant degradation ability

Inactive Publication Date: 2020-05-12
CHINESE RES ACAD OF ENVIRONMENTAL SCI +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ethylene oxide is extremely active, flammable and explosive, and is recognized as a carcinogen in the world, so the barriers to the application of ethylene oxide have been increased.
[0003] At present, there are two main methods for industrial treatment of ethylene oxide waste gas: one is to use sulfuric acid to neutralize it. However, this technology has a small absorption saturation, low treatment efficiency, and produces difficult-to-handle by-products. Increased processing costs
Another method is to use an oxidation reaction furnace for oxidation reaction. This method requires very high control of technical parameters, and the process is prone to explosion.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Enterobacterroggenkampii and application thereof in degradation of ethylene oxide
  • Enterobacterroggenkampii and application thereof in degradation of ethylene oxide
  • Enterobacterroggenkampii and application thereof in degradation of ethylene oxide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Sampling location: Sewage outlet of chemical plant in Foshan City, Guangdong Province

[0038] Sample: Sludge Mixture

[0039] Sampling amount: 10.0g

[0040]Weigh 10.0 g of the sample, add 100 mL of 0.03 mol / L phosphate buffer, mix and clarify for 120 min, and remove large particles of sediment. Draw 1mL of the suspension and add it to 10mL enrichment enrichment medium containing 100mg / L ethylene oxide, and place the inoculated enrichment medium tube and 2.5L anaerobic gas generating bag at the same time in a 2.5L round-bottom stand In a type anaerobic culture bag, seal the culture bag, place it on a shaking table for anaerobic enrichment culture at a rotation speed of 200r / min, anaerobically culture at 37°C for 24-48h, and observe the growth.

[0041] Enrichment and enrichment medium production method:

[0042] Glucose 40g, casein trypsin digest, animal tissue gastric enzyme digest and mix 10g in equal amounts, adjust pH to 5.4-5.8, add distilled water to 1000mL, d...

Embodiment 2

[0050] Ethylene oxide-induced domestication of the original strain EO-10

[0051] Ethylene oxide tolerance acclimatization:

[0052] Using the streaking method, inoculate the original strain of EO-10 ​​on the ethylene oxide tolerance acclimation medium plate containing 100 mg / L ethylene oxide, and culture it anaerobically at a constant temperature at 37°C for 48 hours; select the single cell with the largest colony radius on the plate Colony, subculture to ethylene oxide tolerance acclimation medium plate containing 200mg / L ethylene oxide, 37 ℃ constant temperature anaerobic culture for 48h; select the single colony with the largest colony radius on the plate, subculture to contain 500mg / L ethylene oxide on the ethylene oxide tolerance acclimation medium plate, 37 ℃ constant temperature anaerobic culture for 48h; continue to select the single colony with the largest colony radius on the plate, subculture to 800mg / L ethylene oxide The ethylene oxide tolerance acclimation medi...

Embodiment 3

[0076] Identification of 400mg / L Ethylene Oxide Degradation Effect of EO-10 ​​Strain

[0077] Microbial culture and activation: Take out the original EO-10 ​​strain and EO-10 ​​strain from the -80°C refrigerator, inoculate 10 μL into 100 mL screening purification medium, and culture anaerobically at 37°C, 200 rpm for 48 hours. The concentration of bacteria in the culture solution was 10 10 -10 12 cfu / mL.

[0078] Test group: take 5mL after activation and the concentration is 10 10 -10 12 cfu / mL EO-10 ​​bacterial suspension, inoculated in 400mL ethylene oxide degradation force acclimation liquid medium containing no carbon source but containing 400mg / L ethylene oxide, the number of EO-10 ​​living organisms in the medium was 10 8 -10 10 cfu / mL.

[0079] Control group 1: take 5mL after activation and the concentration is 10 10 -10 12 cfu / mL EO-10 ​​original strain suspension, inoculated in 400mL ethylene oxide degradation force domestication liquid medium containing no ca...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses application of enterobacterroggenkampii EO-10 and application thereof in degradation of ethylene oxide. The collection number of the enterobacterroggenkampii EO-10 is CGMCC No.18440. Microorganisms capable of degrading the ethylene oxide can be screened out effectively, the degradation ability of the degrading strain is improved, and the problem of sewage treatment and discharge in industrial ethylene oxide aftertreatment measures.

Description

technical field [0001] The invention relates to the technical field of biodegradation in the treatment of ethylene oxide waste gas, in particular to a strain of Enterobacter rogerii and its application in degrading ethylene oxide. Background technique [0002] Ethylene oxide is one of the important petrochemical products in the modern chemical industry, and it is a broad-spectrum and highly effective bactericidal disinfectant. Because ethylene oxide can kill most bacteria, spores, viruses and fungi, it has strong penetrating power and can reach the deep layer of objects, which determines its irreplaceable position in the medical sterilization industry. However, ethylene oxide is extremely active, flammable and explosive, and is recognized as a carcinogen in the world, which increases the barriers to the application of ethylene oxide. [0003] At present, there are two main methods for industrial treatment of ethylene oxide waste gas: one is to use sulfuric acid to neutraliz...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N1/36C02F3/34B01D53/84B01D53/72C12R1/01C02F101/34
CPCB01D53/72B01D53/84B01D2251/95C02F3/34C02F2101/34C12N1/36C12N1/205C12R2001/01Y02A50/20
Inventor 刘征涛侯东新薛建龙廖续中王晓南邓瑞珊林佳莉胡胜伟
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com