Yeast sauce and preparation method thereof
A technology of yeast sauce and yeast, which is applied in the field of sauce product processing, to achieve the effects of short production time, high wall breaking rate and small loss of nutrients
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Embodiment 1
[0027] Embodiment 1 brewer's yeast washes broken wall broken wall:
[0028] Add 1 part of beer yeast puree to 2 times the volume and wash it with purified water at 4°C, filter through a 100-mesh filter to remove hop impurities, centrifuge, and wash 1-2 times to obtain the washed yeast puree, and prepare a yeast puree with a concentration of 10-30%. The concentration of sodium chloride is 2-5%. After standing at 40-60°C for 0.5-2h, use APV-2000 high-pressure homogenizer to homogeneously break the wall under the pressure of 110-120Mpa, and cycle the treatment 1-2 times. The cell wall breaking rate is 96- 100%,
[0029] Cell wall breaking rate α=N1 / N2+N2×100%
[0030] Among them: α is the breaking rate of yeast cells; N 1 is the number of cells showing pink color by Gram staining; N2 is the number of cells showing purple color by Gram staining
Embodiment 2
[0031] Enzymolysis and Fresh Extraction of Broken Yeast Mud of Embodiment 2:
[0032] The above-mentioned broken yeast mud, the concentration is 10-20%, adjust the pH value to 7-7.5, add yeast extract enzyme (Guangxi Nanning Pangbo Biological Engineering Co., Ltd.) enzyme amount 3-6.0%, temperature 55-60 ℃, stir the enzyme After 5-12 hours of solution, the DH value of the degree of hydrolysis reaches 20-33%. Heat up to inactivate the enzyme, and vacuum concentrate to a yeast sauce concentration of 60-70%.
Embodiment 3
[0033] Example 3 Enzymolysis of Yeast Mud Maillard Reaction for Flavor and Color Enhancement:
[0034]Add 100 parts of broken wall enzymatic yeast slime, 2 parts of glucose, 2 parts of xylose, 1 part of lysine, 1 part of cysteine hydrochloride, 1 part of glycine, adjust the pH to 6.0-6.5, in the reaction kettle React at 100-120°C for 30-40 minutes to obtain Maillard reaction yeast sauce, which has a rich meat sauce aroma and deep red color. 3-methyl-3-furanthiol was detected by GC-MS. , 3-mercapto-3-pentanone, 1,2-ethanedithiol, 2-ethylpyrazine, 2,4,5-trimethylthiazole, 2-acetylfuran and other flavor compounds. The Maillard reaction type yeast paste is used for flavoring and blending of the final product yeast paste.
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