Yeast sauce and preparation method thereof
A technology of yeast sauce and yeast, which is applied in the field of sauce product processing, to achieve the effects of short production time, high wall breaking rate and small loss of nutrients
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[0027] Example 1 Beer yeast washing and wall breaking:
[0028] Add 1 part of beer yeast mud to 2 times the volume and wash with purified water at 4°C, filter through 100 mesh filter to remove hop impurities, centrifuge and wash 1-2 times to obtain washed yeast mud, and prepare yeast mud with a concentration of 10-30%. Sodium chloride concentration 2-5%, 40-60℃ for 0.5-2h, use APV-2000 high pressure homogenizer at 110-120Mpa pressure to homogenize and break the wall, cyclic treatment 1-2 times, the cell wall breaking rate is 96- 100%,
[0029] Cell wall breaking rate α=N1 / N2+N2×100%
[0030] Among them: α is the rate of yeast cell wall breaking; N 1 is the number of cells that appear pink by Gram stain; N2 is the number of cells that appear purple by Gram stain
Example Embodiment
[0031] Example 2 Enzymatic hydrolysis and extraction of broken yeast mud:
[0032] The above-mentioned broken yeast mud, the concentration is 10-20%, the pH is adjusted to 7-7.5, the enzyme amount of yeast extracting enzyme (Guangxi Nanning Pangbo Biological Engineering Co., Ltd.) is 3-6.0%, the temperature is 55-60℃, the enzyme is stirred After 5-12h, the DH value of hydrolysis degree reaches 20-33%. The temperature is increased to kill the enzyme, and the concentration of the yeast sauce is 60-70% by vacuum concentration.
Example Embodiment
[0033] Example 3 Enzymatically hydrolyzing yeast mud Maillard reaction to increase aroma and color:
[0034] 100 parts of broken wall enzymatic yeast mud add 2 parts of glucose, 2 parts of xylose, 1 part of lysine, 1 part of cysteine hydrochloride, 1 part of glycine, adjust pH 6.0-6.5, in the reactor React at 100-120℃ for 30-40min to obtain Maillard reaction type yeast sauce, with rich meat sauce aroma and deep red color. Detected by GC-MS with GC-MS, 3-methyl-3-furanthiol is detected , 3-mercapto-3-pentanone, 1,2-ethanedithiol, 2-ethylpyrazine, 2,4,5-trimethylthiazole, 2-acetylfuran and other flavor compounds. The Maillard reaction type yeast sauce is used for flavor enhancement and blending of the final product yeast sauce.
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