Application for cotton GhBsr-k1 gene

A cotton and gene technology, applied in the field of genetic engineering, can solve the problems of difficulty, long cycle, and the disease resistance mechanism of cotton Verticillium wilt has not been fully revealed, and achieve the effect of enhancing resistance and reducing direct economic losses.

Pending Publication Date: 2020-05-15
XINJIANG AGRI UNIV
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  • Abstract
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Problems solved by technology

Due to the shortcomings of traditional breeding techniques such as long cycle and high difficulty, the rise of modern genetic engineering technology has greatly improved the efficiency of breeding. At present, there are very few genes that can be used for disease-resistant breeding, and the mechanism of cotton Verticillium wilt disease resistance has not yet been established. Thoroughly revealed, therefore, mining high-quality disease-resistant genes is of great and far-reaching significance to cotton disease-resistant breeding and even the realization of high-yield and disease-resistant cotton

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  • Application for cotton GhBsr-k1 gene
  • Application for cotton GhBsr-k1 gene
  • Application for cotton GhBsr-k1 gene

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Embodiment Construction

[0029] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0030] 1. Materials and methods

[0031] 1. Plant material and treatment

[0032] The plant material to be tested is upland cotton "Junmian No. 1". The seeds with full grains are selected, soaked in 30% hydrogen peroxide solution for 2 hours, rinsed with sterile water for 3 times, soaked in a 37°C incubator for 24 hours, and taken Lubai Put the seeds on the germination paper to germinate. After 1 day, take the seeds with the same bud length and sow them in the nutrient soil containing vermiculite (nutrient soil: vermiculite = 2:1). The cultivation conditions are: 28 ° C ± 2, 16 hours of light ; 2...

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Abstract

The invention discloses an application for a cotton GhBsr-k1 gene. According to the invention, a gene named GhBsr-k1 is cloned from cotton by adopting an RT-PCR method according to the sequence information of a homologous gene; and the gene has a sequence as shown in SEQ ID NO: 1. Results of expression pattern analysis and VIGS-mediated function verification on the gene show that the GhBsr-k1 plays an important negative regulation and control role in cotton verticillium wilt and/or blight resistance; after the GhBsr-k1 gene is silenced, the resistance of the cotton to verticillium wilt and blight is enhanced; the gene is indicated to be able to be used in broad-spectrum disease-resistant breeding of the cotton; and through creation of a cotton mutant material of the Ghbsr-k1, the resistance of the cotton to verticillium wilt and/or fusarium wilt is expected to be greatly enhanced, so the direct economic loss of the cotton industry due to cotton diseases every year is reduced.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to the application of a cotton GhBsr-k1 gene. Background technique [0002] Cotton is a major economic crop in the world, providing renewable fibers and oilseeds for the textile and food industries. Cotton Verticillium wilt is a soil-borne vascular disease caused by Verticillium dahliae Kleb, and it is the primary biotic stress factor that reduces cotton fiber quality and yield. In my country, the cotton field area of ​​cotton Verticillium wilt accounts for 50% of the total planting area, and the direct economic loss caused by Verticillium wilt is as high as 1.6 to 2 billion US dollars every year. Because Verticillium dahliae parasitizes in the vascular bundle of the host, the existence of its microsclerotium and dormant body makes Verticillium wilt not be effectively controlled, and there is no fungicide and planting method that can effectively prevent Vert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H6/60
CPCC12N15/8282C07K14/415
Inventor 李月周垚均代培红任燕萍刘超刘晓东
Owner XINJIANG AGRI UNIV
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