Extraction and purification method for hydrothermally extracting lemon seed total flavonoids and product of method
A purification method and hydrothermal extraction technology, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, anti-toxins, etc., can solve problems such as environmental pollution and waste of resources, and achieve the effect of simple process
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Embodiment 1
[0034] Example 1 standard curve drawing
[0035] Accurately weigh 0.0050g rutin standard product, add appropriate amount of 70% ethanol to dissolve, and dilute to 50mL to obtain rutin standard product solution, respectively accurately draw 1.00, 2.00, 3.00, 4.00, 5.00mL of the above standard product solution into 10mL volumetric flask , that is;
[0036] Add 0.4mL 5% NaNO to the rutin solutions with different concentrations sequentially 2 solution, 0.4mL 10% Al(NO 3 ) 3 Solution, 4mL 4% NaOH solution, add each reagent, let stand for 10min, and finally dilute to 10mL with 70% ethanol.
[0037] Measure its absorbance value at a wavelength of 510nm, take the absorbance value A as the ordinate (Y), and draw the rutin standard curve with the mass concentration of rutin (mg / mL) as the abscissa (X) figure 1 , and calculate the concentration and extraction rate of total flavonoids in subsequent extracts according to the standard curve.
[0038] Calculation formula of extraction r...
Embodiment 2
[0041] 1) Pretreatment: take lemon seeds, wash and dry to constant weight, crush, and the obtained powder is ultrasonically degreased with petroleum ether, and dried by suction filtration. The powder is sieved to 40 mesh;
[0042] 2) Extraction: Weigh 5g of lemon seed powder and place it in a heating container, add ethanol with a volume fraction of 70% and heat at 80°C min, the heating time is 30, 60, 90, 120, 150 min respectively, and the ratio of solid to liquid is 1:10g / mL, filtered to obtain the extract.
[0043] Accurately measure 5.0 mL of the obtained lemon seed extract in a 10 mL volumetric flask, and add 0.4 mL of 5% NaNO 2 solution, 0.4mL 10% Al(NO 3 ) 3 Solution, 4mL 4% NaOH solution, add each reagent, let it stand for 10min, finally use 70% ethanol to make up the volume, measure its absorbance value at 510nm wavelength, according to figure 1 The rutin standard curve calculates the total flavonoid concentration and extraction rate, and the effect of heating tim...
Embodiment 3
[0046] Same as Example 2, the difference is that the heating time is 60min, the ethanol concentration is 70%, the heating temperature is 80°C, and the ratio of solid to liquid is respectively 1:5, 1:10, 1:15, 1:20, 1:25 to obtain the extract .
[0047] Accurately measure 5.0mL of the obtained lemon seed extract in a 10mL volumetric flask, and add 0.4mL 5% NaNO 2solution, 0.4mL 10% Al(NO 3 ) 3 Solution, 4mL 4% NaOH solution, add each reagent, let it stand for 10min, finally use 70% ethanol to make up the volume, measure its absorbance value at 510nm wavelength, according to figure 1 The rutin standard curve calculates the concentration of total flavonoids and the extraction rate, and the effect of the ratio of solid to liquid on the extraction rate of total flavonoids from lemon seeds is shown in image 3 .
[0048] Depend on image 3 It can be seen that the extraction rate increases first and then decreases with the increase of ethanol dosage. In this example, when the e...
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