Method for preparing efficient and stable composite nano-silver bacteriostatic agent from lotus root extract through one-step method

Abstract

The invention relates to the field of preparation of novel bacteriostatic agents, and in particular, relates to a method for preparing a biological mediated composite nano-silver bacteriostatic agent.The method comprises the following steps: 1) washing lotus roots cleanly, slicing, and drying to constant weight to obtain dried lotus root slices; 2) mashing and grinding the dried lotus root slicesinto powder, mixing the powder with an extracting agent according to a mass ratio of 1:(1-100), performing microwave treatment at 100-500 W for 5-10 min, performing heating reflux, centrifuging, taking a supernatant, and placing at the temperature of 4 DEG C for later use; 3) weighing 0.1-3 g of chitosan, adding the chitosan into 100 mL of a 1% glacial acetic acid solution, and continuously stirring overnight to completely dissolve the chitosan for later use; and 4) adding 1-10 mL of the chitosan solution into 30 mL of double distilled water, adding a certain volume of a silver nitrate solution, continuously stirring for 5 min, adding 0.1-10 mL of a lotus root extracting solution, making the volume constant to 50 mL, and performing heating reflux to obtain the composite nano-silver bacteriostatic agent. According to the preparation method, the synergistic bacteriostatic action of the biological nano-silver and the chitosan is fully exerted, a remarkable bacteriostatic effect is achieved, and the composite biological nano-silver preparation prepared by taking the chitosan as the carrier is more stable.

Description

technical field [0001] The invention relates to the field of preparation of novel antibacterial agents, in particular to a preparation method of a biologically mediated composite nano-silver antibacterial agent. Background technique [0002] Lotus root (Nulumbo nucifera Gaertn.) belongs to the perennial aquatic herbaceous plant of Magnoliaceae. Its rhizome can be eaten raw or heated and cooked, and is one of the most widely eaten vegetables. In addition to eating, lotus root also has medicinal value such as clearing heat and promoting body fluid, appetizing and invigorating the spleen, stopping bleeding and removing blood stasis, nourishing blood and invigorating the heart, stopping diarrhea, lowering blood pressure, and lowering blood sugar. Modern scientific research has found that lotus root is rich in polyphenols such as catechin, catechol, gallol, dopa, rutin, chlorogenic acid, and caffeic acid. It also contains organic acids, tannins, polysaccharides, saponins, Vitam...

AI Technical Summary

Problems solved by technology

Although there have been many reports on the biosynthesis of nano-silver using plant and microbial extracts, studies have found that nano-silver antibacterial agents prepared from different biological materials have great differences in physical and chemical properties, antibacterial activity, and antibacterial spectrum. difference

In addition, although amino acids, lipids, proteins, polysaccharides and other substances in biological extracts can stabilize nano-silver to a certain extent, many biosynthetic nano-silver products still have agglomeration during long-term storage. It is not conducive to the promotion and application of nano-silver

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