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Preparation method of antibiotic-free fermented soft granular creep feed for suckling piglets

A technology for teaching trough material and soft granules is applied in the field of preparation of non-fermentative soft granule teaching trough material, which can solve the problems of the influence of suckling piglets, and achieve the effects of improving the breeding ecological environment, good palatability, and inhibiting bacteriostatic or bactericidal effects.

Inactive Publication Date: 2020-06-12
HUNAN LIFENG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the prior art, the present invention provides a preparation method for non-antibiotic fermented soft particle creep feed for suckling piglets, so as to solve the impact of conventional creep feed on suckling piglets in the prior art

Method used

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  • Preparation method of antibiotic-free fermented soft granular creep feed for suckling piglets
  • Preparation method of antibiotic-free fermented soft granular creep feed for suckling piglets

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Strain activation: Spread the selected lactic acid bacteria on the MRS agar solid medium, culture at a constant temperature of 37°C for 24 hours, pick colonies with calcium-dissolving circles, and repeatedly streak to separate pure strains to obtain lactic acid bacteria strains for later use;

[0033] Spread the selected Bacillus subtilis on the solid medium of beef extract peptone agar, incubate at a constant temperature of 37°C for 36 hours, repeatedly streak and isolate the pure strain to obtain the Bacillus subtilis strain, and set aside;

[0034]Inoculate the selected yeast on a potato agar solid culture plate and culture it at 30°C for 3 days, pick a single colony and purify it 2-3 times by streaking on the plate to obtain a yeast strain, and then place it at 4°C Store in the refrigerator for later use.

Embodiment 2

[0036] Preparation of lactic acid bacteria fermentation broth: inoculate the activated lactic acid bacteria strains into 100mL medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 6.0-7.0, the rotation speed is 200-300r / min, and the fermentation time is 24-48h. Shake flask fermentation medium is MRS medium, the ingredients are: peptone 10.0g, Tween-80 1.0ml, beef extract 10.0g, diammonium hydrogen citrate 2.0g, glucose 5.0g, sodium acetate 5.0g, yeast extract 5.0g, MgSO 4 ·7H 2 O 0.2g, MgSO 4 ·H 2 O0.05g, K 2 HPO 4 2.0g, make up 1000mL with distilled water, and adjust the pH to 6.5 with NaOH.

[0037] After the shake flask fermentation is completed, carry out the expansion culture of the fermenter. Take 100mL shake flask fermentation seed liquid and inoculate it into a 10L fermenter. The liquid volume is 5L. 300r / min, fermentation 24-48h. The medium for the pilot test of the fermenter is 2% brown sugar, 0.1% protein, an...

Embodiment 3

[0039] Preparation of Bacillus subtilis fermentation broth: Inoculate the activated Bacillus subtilis strain into 100mL medium for shake flask fermentation culture, the fermentation temperature is 28-37°C, the pH value is 7.2, and the rotation speed is 200-300r / min. The fermentation time is 24-36h. The shake flask fermentation medium is NA medium, and its ingredients are: beef extract 5g, peptone 10g, NaCl 5g, glucose 20g, and distilled water to supplement 1000mL.

[0040] After the shake flask fermentation is completed, the fermenter is expanded. Take 100mL shake flask fermentation seed liquid and inoculate it into a 10L fermenter. The liquid volume is 5L. min, ferment for 24-36h. The composition of the medium in the pilot test of the fermenter was the same as that of the shake flask fermentation medium. After the fermentation, the medium was placed at 4°C for standby to obtain the Bacillus subtilis fermentation broth.

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Abstract

The invention provides a preparation method of an antibiotic-free fermented soft granular creep feed for suckling piglets, and belongs to the technical field of feeds and fermentation. According to the technical scheme, specific bacillus subtilis, saccharomycetes and lactic acid bacteria are selected, the three bacterial strains are fermented respectively to prepare seed solutions, then concentrations of thalli are adjusted respectively, the seed solutions are mixed according to a certain volume ratio and then sprayed and mixed with a suckling piglet granular creep feed without antibiotics and chemical drugs, and bagging, sealing and fermentation are performed. The prepared antibiotic-free fermented soft granular creep feed has an obvious regulating effect on gastrointestinal functions ofthe piglets, and meanwhile, a nutritional value of the feed can be perfected. The feed is directly used as a starter feed for suckling the piglets, and has advantages of good palatability, a high food calling property, a large feed intake, good digestion and absorption, high diarrhea resistance, increase of weight of weaned piglets, reduction of milk fat loss after weaning, and reduction of a diarrhea rate, and therefore a survival rate and a growth rate of the piglets are improved.

Description

technical field [0001] The invention relates to the technical field of animal feed, in particular to a method for preparing antibiotic-free fermented soft granular creep feed for suckling piglets. Background technique [0002] In recent years, with the rapid development of the pig industry, problems such as the deterioration of the breeding environment and the prevalence of epidemic diseases have also emerged. However, in the current large-scale pig farms, especially suckling piglets and nursery piglets, the disease is most frequent. This is because in these two stages, the immune system, digestive system, and thermoregulation system of piglets have not yet fully developed, and their ability to digest and degrade plant proteins and anti-nutritional factors is poor, and their own resistance is low. Severe infection of Haemobacterium, Streptococcus, Escherichia coli, etc. lead to frequent diarrhea of ​​piglets in the farrowing room, joint swelling, weight loss, and panting of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K50/30A23K50/60C12N1/20C12N1/16C12R1/125
CPCA23K50/60A23K50/30A23K10/12C12N1/20C12N1/16
Inventor 陈晓安王刚
Owner HUNAN LIFENG BIOTECH CO LTD
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