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Application of lactobacillus reuteri from breast milk to regulation of maternal and infant immune functions

A technology of Lactobacillus reuteri and Lactobacillus reuteri, applied in the direction of Lactobacillus, application, and the function of food ingredients, to achieve the effect of increasing the depth ratio, enhancing immunity, and promoting the production of intestinal antimicrobial peptides

Active Publication Date: 2020-06-12
FINE HUNAN BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to promote the development of intestinal immunity in the form of probiotic supplements for infants who cannot ingest breast milk or have disordered breast milk flora, as well as pregnant and lactating women who need to enhance immunity , Guarantee intestinal barrier and protective function

Method used

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  • Application of lactobacillus reuteri from breast milk to regulation of maternal and infant immune functions
  • Application of lactobacillus reuteri from breast milk to regulation of maternal and infant immune functions
  • Application of lactobacillus reuteri from breast milk to regulation of maternal and infant immune functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Animal experiment

[0049] 1) Experimental method

[0050] Animal grouping: 40 BALB / C female mice and 14 BALB / C male mice were kept at 8:00-20:00 in the day and night cycle, at a room temperature of 22-24°C, and a humidity of 40-70%, fed with ordinary feed. Separate the male and female to adapt for one week. After one week, they will be caged according to the ratio of female to male = 3:1. After the female is pregnant, the male is taken out. After the female has given birth, the suckling females are divided into two groups, and one group is fed with saline (CON), another group of Lactobacillus reuteri FN041 (1×10 10 CFU / mouse·d), when the pups reach 3 weeks of age, they are weaned naturally, and each group of male pups who have been breast-fed are divided into two groups, and they are fed with normal saline or Lactobacillus reuteri. After 14 days, the treated pups were sacrificed. The above-mentioned mice were grouped into: 1) Con group, both mother and pups wer...

Embodiment 2

[0051] Example 2 Small intestine IgA + Plasma cell immunohistochemical analysis

[0052] (1) Dehydration: Put the small intestine tissues in 50% ethanol; 75% ethanol; 85% ethanol; 90% ethanol for 15 minutes each time, and then put them in 100% ethanol for 30 minutes, and replace the ethanol every 15 minutes.

[0053] (2) Transparent: Prepare a mixture of ethanol and n-butanol at a ratio of 1:1, put the small intestine tissue in the mixture and shake for 20 minutes; discard the liquid, transfer to the n-butanol solution for 40 minutes, and replace the n-butanol every 20 minutes .

[0054] (3) Wax immersion: Immerse in paraffin wax I at 70°C for 3 hours; then transfer to paraffin wax II at 70°C for 3 hours.

[0055] (4) Embedding: Embedding the small intestine tissue processed in step (3) in paraffin.

[0056] (5) Sectioning: Use a Leica microtome to slice 5μm thick jejunum tissue, spread the slices in a water bath at 42℃, and bake slices at 70℃ for 30 minutes.

[0057] (6) Dewaxing and h...

Embodiment 3

[0070] Example 3 Gene expression and antibacterial activity of antimicrobial peptides in small intestinal epithelial cells of young mice:

[0071] The jejunum and small intestine Peyer's knot RNA extraction and cDNA preparation: Take out the liver and spleen of the mouse in the ultra-clean table, cut out 0.1g jejunum and quickly put it into Trizol. The Trizol method was used to extract RNA from jejunum and Peyer's knot. NanoDrop measures OD260 / 280 and concentration, adjusts RNA concentration to 1,000ng / μL, OD 260 / 280 In the range of 1.8-2.0. Perform the reverse transcription of RNA according to the reaction system in Table 1. After mixing the reaction system in the first step, 70℃, 5min, ice bath; after adding the second system and mixing, 37℃ water bath 1h, 95℃, 3min, ice In the bath, the cDNA after reverse transcription is stored at -80°C.

[0072] Jejunal reverse transcription fluorescence quantitative PCR reaction (RT-PCR): Quantitative PCR analysis is performed with jejunal ...

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Abstract

The invention discloses application of lactobacillus reuteri from breast milk to regulation of maternal and infant immune functions, and belongs to the field of microbial technology and food science.The invention provides a lactobacillus reuteri Fn041 for enhancing the immunity of females in the gestation period or the lactation period and infants, namely enhancing the mucosal barrier and promoting the production of intestinal antibacterial peptides and IgA (immunoglobulin A). The lactobacillus reuteri Fn041 has the effects of enhancing the development of an infant immune system, preventing pathogenic bacteria infection, and reducing the incidence of allergic diseases and the like.

Description

Technical field [0001] The invention relates to the application of a strain of lactobacillus reuteri derived from breast milk to regulate the immune function of mothers and infants, belonging to the field of microbial technology and food science, and specifically relates to a lactobacillus reuteri Fn041 combined with breast milk sIgA in promoting the gastrointestinal function of infants and young children. The application of tract immune development and enhancement of adult immunity. Background technique [0002] The initiation and maturation of the human immune system are closely related to intestinal microbes. The bacteria colonized in the intestinal tract of infants and young children play an important role in this process. Breast milk is the main source of the intestinal flora of healthy newborn full-term infants, among which Bifidobacterium and Lactobacillus are the secondary dominant microorganisms. These bacteria can be continuously inoculated into the intestines of infant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P37/04A61P1/00A61P15/00A61P31/04A23L33/135
CPCA61K35/747A61P37/04A61P1/00A61P15/00A61P31/04A23L33/135A23V2002/00A23V2400/173A23V2200/3204A23V2200/324A23L33/105A23L33/185A23L33/17A23L33/19A23L33/21A23L33/40A23V2200/32A23V2200/3202A23V2250/21A23V2250/5062A23V2250/542A23V2250/548A23V2250/55
Inventor 孙进李双祁齐策
Owner FINE HUNAN BIOTECHNOLOGY CO LTD
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