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Surface treatment method of implant and bionic implant

A technology for surface treatment and implants, applied in the fields of biochemical equipment and methods, dental implants, microorganisms, etc., can solve the problems of lack of biological activity and low bonding strength of surrounding tissues with osteoinductive effect.

Active Publication Date: 2020-06-12
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because titanium is an inert metal, it lacks biological activity, and its osteoinductive effect and bonding strength with surrounding tissues are also low.

Method used

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  • Surface treatment method of implant and bionic implant
  • Surface treatment method of implant and bionic implant
  • Surface treatment method of implant and bionic implant

Examples

Experimental program
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Effect test

preparation example Construction

[0048] The embodiment of the preparation method of the above-mentioned bone marrow mesenchymal stem cells can be, for example:

[0049] Aseptically obtain the discarded cancellous bone of the jaw during orthognathic surgery, immediately wash it repeatedly with sterile phosphate buffered saline for 3 to 4 times on the ultra-clean workbench, centrifuge at 800r / min for 5min, discard the supernatant, and use 10% mass Cells were resuspended in α-MEM medium of fractional fetal bovine serum, and inoculated on 25cm 2 Culture flask, placed at 37°C, 5% volume fraction CO 2 cultured in a saturated humidity incubator. When the cells grew confluent and reached 80% of the bottom of the bottle, the cells were digested with 2.5 g / L trypsin and passaged at a ratio of 1:3, which was recorded as P1 (first generation). By analogy, the third generation of human bone marrow mesenchymal stem cells was obtained.

[0050] In one embodiment, the seeding density of bone marrow mesenchymal stem cells ...

Embodiment 1

[0062] The titanium implant to be treated in this embodiment is a pure titanium implant, and the surface treatment method of the titanium implant comprises the following steps:

[0063] S100: Titanium implants are provided, with titanium implants as the anode and pure copper as the cathode for anodic oxidation, the electrolyte is a mixture of 0.15mol / L ammonium fluoride and 0.5mol / L ammonium sulfate, and the voltage for anodic oxidation is 22V , time 30min; forming titanium dioxide nanotubes on the surface of the titanium implant.

[0064] S200: The anodized titanium implant was ultrasonically cleaned with double distilled water, dried, sterilized under high temperature and high pressure, and then irradiated with an ultraviolet lamp for 30 minutes to obtain an activated titanium implant.

[0065] S210: Aseptically obtain the discarded cancellous bone of the jaw during orthognathic surgery, immediately wash it repeatedly 4 times with sterile phosphate buffer saline on the ultra...

Embodiment 2

[0071] The titanium implant to be treated in this embodiment is a titanium alloy implant, and the surface treatment method of the titanium implant comprises the following steps:

[0072] S100: Titanium implants are provided, with titanium implants as the anode and pure copper as the cathode for anodic oxidation, the electrolyte is a mixture of 0.16mol / L ammonium fluoride and 0.55mol / L ammonium sulfate, and the voltage of anodic oxidation is 20V , for 35 minutes; forming titanium dioxide nanotubes on the surface of the titanium implant.

[0073] S200: The anodized titanium implant was ultrasonically cleaned with double distilled water, dried, sterilized under high temperature and high pressure, and irradiated under an ultraviolet lamp for at least 30 minutes to obtain an activated titanium implant.

[0074] S260: Aseptically obtain the discarded cancellous bone of the jaw during orthognathic surgery, immediately wash it repeatedly 4 times with sterile phosphate buffer saline on...

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Abstract

The invention relates to a surface treatment method of an implant and a bionic implant. The surface treatment method comprises the following steps of: providing a titanium implant, performing anodic oxidation on the titanium implant, and forming titanium dioxide nanotubes on the surface of the titanium implant; cleaning the anodized titanium implant, drying, sterilizing at high temperature and high pressure, and irradiating under an ultraviolet lamp to obtain an activated titanium implant; providing bone marrow mesenchymal stem cells, and respectively inoculating the bone marrow mesenchymal stem cells on the surface of the activated titanium implant; adding an alpha-MEM culture medium containing 10% FBS, culturing in an incubator for at least 2 days, adding an osteogenic induction solutionfor culturing, and inducing for at least 14 days to obtain an initial product; and carrying out decellularization treatment on the initial product. An environment similar to the osteogenesis state ofthe human body is built on the surface of the titanium implant, the biological function of the titanium implant is increased, and the bionic effect is achieved.

Description

technical field [0001] The invention relates to the technical field of stomatology and dentistry, in particular to a surface treatment method of an implant and a bionic implant. Background technique [0002] The extracellular matrix is ​​secreted by cells and accumulated around it. It is a network complex formed by molecular particles with various structures and functions. Biological microenvironment, and by regulating the activity of signaling molecules, affect cell morphology, proliferation, migration and differentiation and other cell behaviors. In bone tissue engineering, a large number of studies have used the biological characteristics of extracellular matrix to synthesize bone substitute materials with certain biological functions. Various components in the extracellular matrix have a certain regulation or promotion effect on the osteogenesis of surrounding cells. Some scholars load extracellular matrix components such as collagen, hydroxyapatite or other active subs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61C8/00
CPCC12N5/0663A61C8/0093C12N2533/00
Inventor 王安训吴彧
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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