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Fluorescent probe for detecting hypochlorite in water environment as well as preparation method and application thereof

A fluorescent probe and yellow fluorescence technology, applied in the field of nanomaterial preparation and application, can solve the problems of low detection sensitivity, poor water solubility, low quantum yield, etc., and achieve the effects of high sensitivity, high selectivity and good reproducibility

Pending Publication Date: 2020-06-16
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But currently detection of ClO - Most of the carbon quantum dots are green fluorescent carbon quantum dots, which have the disadvantages of poor water solubility and low quantum yield, which lead to the problem of low detection sensitivity, which limits their application in the fields of chemical sensing and biological detection.

Method used

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  • Fluorescent probe for detecting hypochlorite in water environment as well as preparation method and application thereof
  • Fluorescent probe for detecting hypochlorite in water environment as well as preparation method and application thereof
  • Fluorescent probe for detecting hypochlorite in water environment as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A yellow fluorescent carbon quantum dot fluorescent probe, such as Figure 13 Shown, its preparation method comprises the following steps:

[0050] 1) Take 0.1mmol of p-phenylenediamine, 2ml of tetraethylene glycol, 50ml of absolute ethanol and 0.5ml of concentrated sulfuric acid, sonicate for 10min at room temperature, and the ultrasonic power is 100W, the color of the solution changes from light red to white, Obtain mixed reaction solution;

[0051] 2) Add the mixed reaction solution into the autoclave, and carry out carbonization treatment on the reaction solution in the kettle by solvothermal method at a stirring speed of 180°C and 400 r / min for 8 hours to obtain the carbonized mixed solution;

[0052] 3) Filter the carbonized mixture in sequence (0.22μm canned syringe filter), concentrate the filtrate (rotary evaporator, the volume after concentration is 3-5mL), and separate on a silica gel column (eluent is petroleum ether and ethyl acetate equal volume mixture)...

Embodiment 2

[0058] This embodiment is used to investigate the test water sample pH value, NaCl concentration, UV lamp irradiation time and storage time. - The impact of the detection effect includes the following steps:

[0059] 1) Dissolve the solid yellow fluorescent carbon quantum dot fluorescent probe prepared in Example 1 in deionized water to obtain a concentration of 0.01 mg·mL -1 Y-CQDs mother liquor;

[0060] 2) preparing hydrochloric acid or sodium hydroxide into aqueous solutions with different pH values ​​(1-12);

[0061] 3) Take multiple centrifuge tubes, add 30 μL Y-CQDs mother solution and 30 μL ClO to each centrifuge tube respectively - Standard solution (7.2μM), then diluted to 4mL by adding aqueous solutions of different pH values, then rapidly stirred at room temperature for 10s, then transferred to a 4mL quartz cuvette, detected by a fluorometer at regular intervals and recorded the fluorescence intensity.

[0062] The result is as follows:

[0063] like Figure 4...

Embodiment 3

[0066] In this embodiment, the yellow fluorescent carbon quantum dot fluorescent probe prepared in Example 1 is used to detect ClO in unknown solution - Concentration, specifically including the following steps:

[0067] 1) Prepare pH=4, 10mM Na 2 HPO 4 - citric acid buffer solution;

[0068] 2) Take 0.01g of the yellow fluorescent carbon quantum dot fluorescent probe prepared in Example 1 and dissolve it in 1000mL deionized water to prepare 0.01mg·mL -1 Y-CQDs mother liquor;

[0069] 3) Prepare 0.4mM ClO - solution;

[0070] 4) Take 0, 10, 20, 30, 40, 50, 60, 70, 80 and 360 μL of 0.4 mM ClO - solution, respectively mixed with 30 μL Y-CQDs mother solution, and then added Na 2 HPO 4 - The citric acid buffer solution was diluted to 4 mL, then rapidly stirred at room temperature for 10 s, then transferred to a 4 mL quartz cuvette, and the fluorescence intensity was detected and recorded by a fluorometer (parameter settings: excitation wavelength 410 nm, emission waveleng...

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Abstract

The invention relates to a yellow fluorescent carbon quantum dot fluorescent probe and a preparation method and application thereof. The preparation method comprises the steps that: p-phenylenediamine, tetraethylene glycol, ethyl alcohol and concentrated sulfuric acid are mixed, a mixed reactant is carbonized through a solvothermal method, and then the yellow fluorescent carbon quantum dot fluorescent probe is obtained after separation and purification; the prepared yellow fluorescent carbon quantum dot fluorescent probe can be applied to detection of ClO<-> ions in a water environment. Compared with the prior art, the yellow fluorescent carbon quantum dot fluorescent probe provided by the invention has the advantages of simple and mature preparation method, rapid detection response, highselectivity, strong anti-interference performance, good reproducibility and the like, and has good application prospect and practical value in the fields of environment, chemistry, biology, medicine and the like.

Description

technical field [0001] The invention belongs to the technical field of nanomaterial preparation and application, and relates to a fluorescent probe for detecting hypochlorite in water environment and its preparation method and application, in particular to a yellow fluorescent carbon quantum dot for detecting hypochlorite Fluorescent probe, its preparation method and its application in the fluorescence detection of hypochlorite. Background technique [0002] Hypochlorite is widely used in daily life and industrial production, such as household bleaching, drinking water disinfection, cooling water treatment, and oxidation reactions. Hypochlorite is a very important active oxygen that exists in living tissues and often plays an important role in the immune system. Hypochlorite can react with a variety of biomolecules, including fatty acids, cholesterol, and proteins. However, high-concentration hypochlorite solution not only poses a potential threat to humans and animals, bu...

Claims

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Application Information

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IPC IPC(8): C09K11/65B82Y20/00B82Y30/00G01N21/64
CPCC09K11/65B82Y20/00B82Y30/00G01N21/643G01N2021/6417
Inventor 李明中徐虎梅杰方振徐冬梁玉婷王洪阳王宇红
Owner SHANGHAI INST OF TECH
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