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Nucleic acid aptamer infrared spectrum sensor and detection method for detecting atz

A technology of nucleic acid aptamer and infrared spectroscopy, which is applied in the field of nucleic acid aptamer infrared spectroscopy sensor and detection of ATZ, can solve the problems of complicated operation process, low detection sensitivity, and long analysis period, so as to enhance sensitivity and improve selection sex, avoidance of distraction effects

Active Publication Date: 2021-03-26
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the analysis and detection methods of ATZ mainly include instrumental analysis methods (HPLC, GC-MS, HPLC-MS, etc.), electrochemical sensing analysis, photoelectric analysis methods, etc., but traditional instrumental analysis methods often have cumbersome sample pretreatment. , long analysis period, complex operation process, and low detection sensitivity, while electrochemical and photoelectrochemical analysis have solved the problems of low detection sensitivity and complex operation in instrumental analysis to a certain extent, but it is still difficult to achieve molecular Horizontal real-time quantitative analysis and detection of target objects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation method of the nucleic acid aptamer infrared spectrum sensor for detecting atrazine is as follows:

[0038] (1) Pretreatment of Si prisms. The semi-cylindrical prism with a reflective surface of 2cm x 2.5cm needs to be pre-treated to ensure the surface is clean before it can be used as an infrared window. On a metallographic polishing machine, Al with a particle size of 1.0 μm, 0.3 μm, and 0.05 μm 2 o 3 Si prisms were physically polished (650 rpm) with polishing powder until the surface was bright and completely hydrophobic. The polished Si prisms were ultrasonically cleaned in water, acetone, and water in sequence, and then placed in freshly prepared piranha solution (7:3 volume ratio of H 2 SO 4 and H 2 o 2 Soak in mixed solution), wait for it to cool to room temperature, pour out the lotion, and then use high-purity water to ultrasonically clean it. 2 Blow dry under atmosphere. Si prisms with a hydrophilic surface were placed in 40% NH 4 Soak ...

Embodiment 2

[0045] The MCH / Aptamer / Au NPs / Si prism infrared spectrum sensor prepared in Example 1 was used for infrared spectrum detection of ATZ.

[0046] Use liquid nitrogen to cool the MCT detector, set the incident angle of the Au nano film / solution interface θ = 70° to ensure that the infrared radiation is completely reflected. 2 Under the atmosphere, add PBS buffer (50mM) to the surface of the sensor to buffer for 30min, collect the infrared spectrum curve, the spectral range is 600-4000cm -1 After the background was collected, a series of ATZ standard solutions with different concentrations were added to the PBS solution for 60 minutes to make ATZ adsorb on the surface of the sensor in saturation, and then the infrared spectrum was measured. Through the analysis results, it was found that ( figure 2 As shown in A), within a certain concentration range of ATZ, with the increase of the concentration of ATZ, the intensity of the characteristic infrared peak of ATZ also increases cor...

Embodiment 3

[0048] The MCH / Aptamer / Au NPs / Si prism infrared spectrum sensor prepared in Example 1 was used for selective performance detection.

[0049] Use liquid nitrogen to cool the MCT detector, set the incident angle of the Au nano film / solution interface θ = 70° to ensure that the infrared radiation is completely reflected. 2 Under the atmosphere, add PBS buffer (50mM) to the surface of the sensor to buffer for 30min, collect the infrared spectrum curve, the spectral range is 600-4000cm -1 . Then other interfering substances (glyphosate, acetamiprid) were added to the PBS buffer solution for 60 minutes, and the infrared spectrum was measured under the same conditions to study the intensity change at the ATZ characteristic absorption peak position, and to investigate the infrared spectrum of nucleic acid aptamers. Selective properties of spectral sensors. The results show that when other interferences are used instead of ATZ for infrared spectrum measurement, there is no obvious pe...

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Abstract

The invention relates to a nucleic acid aptamer infrared spectrum sensor for detecting ATZ and a detection method. The preparation method of the sensor comprises the following steps: depositing au NPson the surface of the Si prism to provide an enhanced infrared absorption signal; and then combining the atrazine aptamer to the surface of an infrared detection substrate through an Au-S bond self-assembly method, and finally blocking residual sites by utilizing MCH to prepare the MCH / Aptamer / Au NPs / Si aptamer infrared spectrum sensor. Compared with the prior art, according to the invention, thenucleic acid aptamer is used as a recognition element, the selectivity of the sensor is greatly improved, an attenuated total reflection infrared absorption spectrum is used as a quantitative detection basis, so the structure information can be provided, the near field effect and the surface enhancement effect can effectively enhance the detection sensitivity of the sensor, the detection on the molecular level can be achieved, the detection limit is as low as 30 pM, and the detection method is rapid, simple and convenient, and can be used for the detection analysis of trace organic pollutants.

Description

technical field [0001] The invention belongs to the technical field of environmental pollutant analysis and spectral analysis, and relates to a nucleic acid aptamer infrared spectrum sensor and a detection method for detecting ATZ. Background technique [0002] Triazine herbicides are the largest category of pesticide endocrine disruptors. The large amount of residues of this compound and its degradation products in water bodies have caused great threats and damage to the health of organisms and the ecological environment. Among them, atrazine (ATZ) is considered to be the most polluting triazine herbicide due to its large usage, strong stability, and long residual time in water. The accumulation of ATZ in the body will seriously It affects the central nervous system, endocrine system and immune system, and has potential carcinogenicity. All countries in the world have made clear limit standards for ATZ residues in crops, soil and surface water. my country's "Environmental Qu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/3577G01N21/552G01N27/48G01N27/42
CPCG01N21/3577G01N21/554G01N27/42G01N27/48
Inventor 刘梅川孙欢欢孙彩琴丁雪赵国华
Owner TONGJI UNIV
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