Method for evaluating honeysuckle characteristics based on marker flavonoids screened by metabolome

A technology of flavonoids and honeysuckle is applied in the field of labeling flavonoids to evaluate honeysuckle and its processed products, which can solve the problems of limited research throughput, inability to efficiently, systematically and comprehensively characterize, and achieve the effect of broad application prospects and rapid repeatability.

Active Publication Date: 2020-06-26
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been some reports on the extraction, separation and purification of honeysuckle flavonoids, but most of the existing studies have limited throughpu

Method used

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  • Method for evaluating honeysuckle characteristics based on marker flavonoids screened by metabolome
  • Method for evaluating honeysuckle characteristics based on marker flavonoids screened by metabolome
  • Method for evaluating honeysuckle characteristics based on marker flavonoids screened by metabolome

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 preparation of flavonoid extract

[0038] (1) Collection of honeysuckle flower buds / flowers at different developmental stages

[0039] According to previous reports (Xiaofei Shang et al., 2011, Journal of Ethnopharmacology), the formation of honeysuckle flower buds is divided into six representative periods, namely the juvenile bud stage, the third green stage and the third green stage. stage), the second white stage, the completewhite stage, the silver flowering stage and the gold flowering stage. According to reports by other researchers (Jie Wu et al., 2015, Scientific Reports), the juvenile bud stage can be subdivided into the Milei stage and the early bud stage. The specific morphological characteristics are shown in Table 1.

[0040] Table 1 Morphological characteristics of Lonicera japonica at different stages of flower development

[0041]

[0042]

[0043] Collect and weigh the honeysuckle flower buds / flowers ( figure 1 ) 2-3g, three biol...

Embodiment 2

[0046] Example 2 Detection of Honeysuckle Flavonoids Samples Based on LC-MS / MS

[0047] The instrumentation system for data acquisition includes ultra-high performance liquid chromatography and tandem mass spectrometry.

[0048] Liquid phase conditions mainly include: ① Chromatographic column: Waters ACQUITY UPLC HSS T3 C18 1.8 μm; ② Mobile phase A: ultrapure water (containing 0.04% acetic acid by volume); Mobile phase B: acetonitrile (containing 0.04% acetic acid by volume); ③Elution gradient: 0-11min, 95-5% mobile phase A, 5-95% mobile phase B; 11-12min, 5% mobile phase A, 95% mobile phase B; 12-12.1min, 5-95% Mobile phase A, 95-5% mobile phase B; 12.1-15min, 95% mobile phase A, 5% mobile phase B; ④ flow rate 0.3-0.4mL / min; column temperature 40°C; injection volume 2μL.

[0049] The mass spectrometry conditions mainly include: the temperature of the electrospray ion source is 500°C; the mass spectrometry voltage is 5500 V; the curtain gas is 25 psi; the collision-induced io...

Embodiment 3

[0050] Example 3 Analysis of detection results of honeysuckle flower buds / flowers flavonoids

[0051] (1) Qualitative and quantitative analysis of flavonoid metabolites

[0052] Mass spectrometry data were processed using the software Analyst 1.6.3. Mainly based on the public database of metabolite information, the qualitative analysis of the first-order spectrum and second-order spectrum data detected by mass spectrometry is carried out. Metabolite structure analysis refers to existing public mass spectrometry databases such as MassBank, KNAPSAcK, HMDB, MoTo DB, and METLIN. The characteristic ions of each substance were screened out by the triple quadrupole, the signal intensity (CPS) of the characteristic ions was obtained in the detector, and the mass spectrum file of the sample was opened with MultiaQuant software. After obtaining the metabolite mass spectrometry data of different samples, the peak area integration of all the mass spectrum peaks of the substance is perfo...

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Abstract

The invention relates to a method for evaluating honeysuckle characteristics based on marker flavonoids screened by metabolome. According to the invention, the method comprises the steps: employing the flavonoid components and the content of the flavonoid components of flower buds/flowers of honeysuckle in different development stages by virtue of an LC-MS/MS technology; quantitatively analyzing the flavonoid difference in different development stages and screening the labeled flavonoid substances in each development stage of the medicinal bud stage, so as to judge the flavonoid category and content of the fresh honeysuckle raw material. The screened formononetin and 7-oxomethyl quercetin can be used as specific marker flavonoid substances in the optimal medicinal bud stage (the second white stage); the 2, 6-gingerol can be used as a specific marker flavonoid substance in the three-green stage, and the peach aglycone can be used as a specific marker flavonoid substance in the large white stage. The invention also provides a basis for extraction and quantitative detection of honeysuckle flavonoids of different cultivated varieties in different cultivation regions, and provides an evaluation method for judging the flavonoid substance characteristics of fresh honeysuckle samples.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicinal plant detection, relates to the field of flavonoid extraction technology of plant flower organs, instrument analysis technology and data analysis technology, and in particular to the detection and screening of flavonoid markers in the flower bud stage of honeysuckle based on LC-MS / MS technology , using labeled flavonoids to evaluate honeysuckle and its processed products. Background technique [0002] Flavonoids (flavonoids), also known as flavonoids, are a group of compounds that exist in nature. Flavonoids are secondary metabolites produced by the phenylalanine metabolic pathway. Most plants contain flavonoids, which play an important role in plant growth, development, and stress resistance. Flavonoids also achieve anti-bacterial, anti-viral, anti-inflammatory, anti-allergic, protection against UV damage and inhibition of lipid peroxidation, platelet aggregation, and capil...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72
CPCG01N30/02G01N30/06G01N30/30G01N30/32G01N30/34G01N30/72G01N2030/324
Inventor 夏燕薛宝贵梁国鲁郭启高景丹龙刘新亚石敏
Owner SOUTHWEST UNIVERSITY
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