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A kind of nano silicon dioxide/decomposition enzyme/polycaprolactone composite microsphere and its preparation method and application

A technology of nano-silica and polycaprolactone, applied in the field of medical materials, can solve the problems of easy-to-failure enzymes, accelerate the degradation rate of polylactic acid, and inactivation

Active Publication Date: 2021-11-16
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that decomposing enzymes (such as lipase, K-protease, etc.) can significantly accelerate the degradation rate of polylactic acid under the condition of ensuring activity, and decomposing enzymes have many advantages such as non-toxic, environmentally friendly, and cheap, but lipase added to polylactic acid Lactic acid fiber is prone to failure and enzyme inactivation

Method used

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  • A kind of nano silicon dioxide/decomposition enzyme/polycaprolactone composite microsphere and its preparation method and application
  • A kind of nano silicon dioxide/decomposition enzyme/polycaprolactone composite microsphere and its preparation method and application
  • A kind of nano silicon dioxide/decomposition enzyme/polycaprolactone composite microsphere and its preparation method and application

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preparation example Construction

[0041] The present invention provides a method for preparing the composite microspheres described in the above scheme, comprising the following steps:

[0042] (1) Mixing nano-silica, decomposing enzyme, anionic surfactant, dispersant and water, stirring the obtained mixed solution, ultrasonic treatment and static adsorption in order to obtain a silica / decomposing enzyme complex;

[0043] (2) mixing the silica / decomposing enzyme complex, anionic surfactant, dispersant and water to obtain a silica / decomposing enzyme complex dispersion;

[0044] (3) adding the silica / decomposing enzyme complex dispersion into the polycaprolactone solution for the first emulsification treatment to obtain the first emulsion; the solvent of the polycaprolactone solution is dichloromethane;

[0045] (4) adding the first emulsion to a mixed solution of a dispersant and an anionic surfactant to perform a second emulsification treatment to obtain a second emulsion;

[0046] (5) remove the dichlorometh...

Embodiment 1

[0063] Reagents: polycaprolactone, molecular weight 45,000, Natureworks, USA; nano-silica, Jiangsu Tianxing New Material Co., Ltd., particle size: 20nm; lipase, Sigma, USA, L3126, activity: 30-90U / mg; polyethylene Diol, molecular weight 4000, pharmaceutical grade, Shandong Youso Chemical Technology Co., Ltd.; sodium dodecylbenzenesulfonate, dichloromethane, phosphate buffer (Hubei Wanye Pharmaceutical Co., Ltd., pH7.4, reagent grade).

[0064] Preparation steps:

[0065] (1) Preparation of nano-silica / lipase complex

[0066] Weigh 0.5g of nano-silica, 0.5g of lipase, 0.5g of sodium dodecylbenzenesulfonate and 2g of polyethylene glycol and dissolve them in water to form a 500mL mix of nano-silica / lipase solution. The prepared above-mentioned solution was placed in an intelligent digital display magnetic electric heating mantle for magnetic stirring for 15 minutes, and then placed in an ultrasonic cleaner for ultrasonic treatment for 20 minutes. Stand still for 24 hours to co...

Embodiment 2

[0084] The other steps are the same as in Example 1, only controlling the concentration of the nano-silica / lipase complex dispersion in step (2) to be 0.3 mg / mL to obtain the core material and capsule wall quality Composite microspheres with a ratio of 3:50;

[0085] Other steps are the same as in Example 1, only controlling the concentration of the nano-silica / lipase complex dispersion in step (2) to be 0.5mg / mL to obtain the core material and capsule wall quality Composite microspheres with a ratio of 5:50;

[0086] The other steps are the same as in Example 1, only controlling the concentration of the nano-silica / lipase complex dispersion in step (2) to be 0.7mg / mL to obtain the core material and capsule wall quality Composite microspheres with a ratio of 7:50;

[0087] The other steps are the same as in Example 1, only controlling the concentration of the nano-silica / lipase complex dispersion in step (2) to be 0.9 mg / mL to obtain the core material and capsule wall mass ...

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Abstract

The invention relates to the technical field of medical materials, and provides a nano-silica / decomposition enzyme / polycaprolactone composite microsphere and its preparation method and application. The composite microsphere provided by the invention includes a capsule wall and a capsule coated The core material inside the wall; the capsule wall is polycaprolactone, and the core material is a nano-silica / decomposing enzyme complex, and the decomposing enzyme includes lipase and / or K-protease. In the present invention, the decomposing enzyme is fixed on the surface and pores of the nano-silica, which can protect the activity of the decomposing enzyme, and a layer of polycaprolactone capsule wall is coated on the outer surface of the nano-silica / decomposing enzyme complex. The lactone capsule wall temporarily encapsulates the nano-silica / decomposing enzyme complex, avoiding direct contact between the decomposing enzyme and various solvents during processing, and protecting the activity of the decomposing enzyme; in addition, polycaprolactone is biodegradable With the degradation of the capsule wall, the decomposing enzymes are released, which play a role in regulating the degradation rate of the aliphatic polymer.

Description

technical field [0001] The invention relates to the technical field of medical materials, in particular to a nano-silica / decomposing enzyme / polycaprolactone composite microsphere and a preparation method and application thereof. Background technique [0002] Aliphatic polymers have good biocompatibility and biodegradability, can eventually degrade into carbon dioxide and water, and have high mechanical strength. They are widely used as controlled drug release materials, surgical sutures, and medical dressings. [0003] At present, polylactic acid is the most commonly used aliphatic polymer in the medical field. Polylactic acid (PLA) fiber (also known as corn fiber) is made from starchy plants such as corn and cassava. After fermentation, polymerization, A new type of polyester fiber made by spinning has many advantages such as high mechanical strength, complete biodegradability, and good biocompatibility. It is considered to be one of the most promising biodegradable materia...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L15/18A61L15/38A61L15/26A61L15/42A61L15/62A61L15/44A61L17/12A61L17/00
CPCA61L15/18A61L15/26A61L15/38A61L15/42A61L15/44A61L15/62A61L17/00A61L17/005A61L17/12A61L2300/254A61L2300/622A61L2400/12C08L67/04
Inventor 刘淑强吴改红刘明芳李甫张曼张瑶王鹏武捷张洁靖逸凡
Owner TAIYUAN UNIV OF TECH
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