Gastridin-17 detection kit, and preparation method and detection application method thereof

Abstract

The invention discloses a gastrin-17 detection kit which comprises a component I, a component II and a gastrin-17 standard substance; the component I adopts an anti-gastrin-17 first antibody coated with magnetic spheres; the component II adopts an anti-gastrin-17 second antibody directly or indirectly marked by a near-infrared fluorescent dye; the anti-gastrin-17 first antibody, the anti-gastrin-17 second antibody and gastrin-17 in a to-be-detected sample respectively have binding sites; the gastrin-17 standard substance is used for making a gastrin-17 standard solution fluorescence signal calibration curve; the fluorescence signal intensity of the to-be-detected sample is detected; the concentration of the gastrin-17 in the to-be-detected sample is calculated according to the gastrin-17 standard solution fluorescence signal calibration curve. The invention also relates to a preparation method and a detection application method of the gastrin-17 detection kit. According to the gastrin-17 detection kit, and the preparation method and detection application method thereof of the invention, a double-antibody sandwich immunoassay principle is utilized, and a magnetic bead extraction separation technology and near-infrared dye labeling characteristics are creatively combined, so that the concentration of gastrin-17 can be rapidly, accurately and sensitively determined.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and specifically relates to a gastrin-17 detection kit, and also relates to a preparation method and a detection application method of the gastrin-17 detection kit. Background technique [0002] Gastric cancer is a malignant tumor of the digestive system, characterized by high morbidity and mortality, and has become one of the most common diseases that endanger human health and safety. Early diagnosis of gastric cancer is the most effective way to reduce the mortality rate associated with this disease. However, since early gastric cancer is asymptomatic or non-specific, it is difficult to diagnose early, so non-invasive examinations that can diagnose early gastric cancer are urgently needed. Atrophic gastritis is an extremely important cancerous disease of the former stomach, usually caused by the bacterium Helicobacter pylori. Atrophic gastritis results in loss of glandular archit...

AI Technical Summary

Problems solved by technology

Chemiluminescence immunoassay mainly uses the horseradish peroxidase (HRP) system, but the working curve of the luminescence reaction catalyzed by horseradish peroxidase may drift with time, the peak of the luminescence of high concentration samples decays quickly, and the low-end slope It is easy to move down linearly, resulting in poor measurement accuracy

However, the detection wavelength of visible fluorescence immunoassay is in the visible light region (400-650nm), and biological samples such as cells and tissues have strong autofluorescence in this band, so the detection results are more seriously affected by background fluorescence interference.

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